Analytical Science and Technology (분석과학)

The Korean Society of Analytical Sciences (한국분석과학회)
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Quality inspections of active components in oxidative hair coloring products by UPLC

UPLC를 이용한 염모제품에 함유된 유효성분에 대한 품질검사

  • Kim, Dong-Gyu (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Kim, Yoo-Kyung (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Yun, Eun-Sun (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Hong, Mi-Sun (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Shin, Ji-Young (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Jeong, Yoon-Kyung (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Kim, Jung-Hun (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Chae, Young-Zoo (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Park, Seung-Kook (Department of Food Science and Biotechnology, Institute of Life Science and Resources, Kyung Hee University)
  • 김동규 (서울시 보건환경연구원) ;
  • 김유경 (서울시 보건환경연구원) ;
  • 윤은선 (서울시 보건환경연구원) ;
  • 홍미선 (서울시 보건환경연구원) ;
  • 신지영 (서울시 보건환경연구원) ;
  • 정윤경 (서울시 보건환경연구원) ;
  • 김정헌 (서울시 보건환경연구원) ;
  • 채영주 (서울시 보건환경연구원) ;
  • 박승국 (경희대학교 생명과학대학)
  • Received : 2012.10.18
  • Accepted : 2013.01.25
  • Published : 2013.02.25
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Abstract

To identify oxidative hair dyes in hair-coloring products, the thin-layer chromatography (TLC) screening method was used in accordance with Korean Quasi-drug Codex. However, the TLC method is not reliable when there are very small amount of materials to be tested or when $R_f$ values of several components are similar. In this study, Ultra Performance Liquid Chromatography (UPLC) with a rapid sample preparation method was developed for the reliable and sensitive identification of active components contained in oxidative hair-coloring products. Hexane-distilled water was used for the extraction of active components contained in the products prior to UPLC analysis. The limit of detection of active components was 6.7-77.9 ${\mu}g/L$, and the limit of quantitation was 22.3-259.7 ${\mu}g/L$. Except for ${\alpha}$-naphthol, the range of recovery ratio was 96.2-101.5%. From this study, we demonstrated that oxidative active hair-coloring components can easily be analyzed by rapid extraction method followed by UPLC analysis.

염모제품 중 유효성분의 품질검사는 식품의약품안전청의 "의약외품에 관한 기준 및 시험방법"에 따라 제품에 표기된 산화염료들을 박층크로마토그래프법(TLC방법)으로 확인시험을 하도록 되어있다. 그러나 TLC방법은 원료분량이 미량이거나, $R_f$ 값이 비슷한 성분들이 존재하면 확인시험이 어려운 문제점이 있다. 본 연구에서는 미량성분 검출이 용이하며 분석시간이 짧다고 보고된 UPLC를 이용하기 위한 시료 전처리 및 분석조건을 탐색하였다. 유효성분들을 분석할 수 있는 검출한계는 6.7-77.9 ${\mu}g/L$, 정량한계는 22.3-259.7 ${\mu}g/L$ 이었으며, 회수율은 ${\alpha}$-naphthol를 제외하고는 96.2-101.5%로 양호하였다. 유효성분 추출하기 위한 시료 전처리에는 헥산-증류수를 사용하였다. 시료 전처리 시 복잡한 추출과정을 거치지 않고, UPLC방법은 빠르고 정확하게 염모제품에 함유되어 있는 유효성분들을 동시에 분석 할 수 있었다.

Keywords

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