Journal of the Optical Society of Korea

  • 제17권1호
  • /
  • Pages.81-85
  • /
  • 2013
  • /
  • 1226-4776(pISSN)
  • /
  • 2093-6885(eISSN)
한국광학회 (Optical Society of Korea)
권호 표지
DOI QR코드

DOI QR Code

Miniaturized Fluorometer Based on Total Internal Reflector and Condensing Mirror

  • Jang, Dae-Ho (College of information and Communication Engineering, Sungkyunkwan University) ;
  • Yoo, Jae-Chern (College of information and Communication Engineering, Sungkyunkwan University)
  • 투고 : 2012.09.28
  • 심사 : 2012.12.17
  • 발행 : 2013.02.25
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

A miniaturized fluorescence detection system based on total internal reflection (TIR) configuration, which is applicable to detecting the presence of biological materials labeled with fluorescence dye in micro total analysis systems (${\mu}TAS$), is proposed. In conventional fluorescence testing and analysis devices, interference between the excitation light beam and the emitted light from dyes is unavoidable. This paper presents a fluorescence detection system based on TIR configuration that allows the excitation light beam and the emitted light to be spatially perpendicular to each other so as to minimize the interference where fluorescence emission is detected at the orthogonal angle to the excitation beam. We achieved the limit of detection of about 5 nmol/L with a high linearity of 0.994 over a wide range of 6-FAM mol concentration, being comparable to that in earlier studies.

키워드

참고문헌

  1. J. F. Gouin, A. Doyle, and B. D. MacCraith, "Fluorescence capture by planar waveguide as platform for optical sensors," Electron. Lett. 34, 1685-1687 (1998). https://doi.org/10.1049/el:19981124
  2. J. R. Webster, M. A. Burns, D. T. Burke, and D. H. Mastrangelo, "Monolithic capillary electrophoresis device with integrated fluorescence detector," Anal. Chem. 73, 1622-1626 (2001). https://doi.org/10.1021/ac0004512
  3. O. Hofmann, X. H. Wang, J. C. deMello, D. D. C. Bradley, and A. J. deMello, "Towards microalbuminuria determination on a disposable diagnostic microchip with integrated fluorescence detection based on thinfilm organic light emitting diodes," Lab Chip 5, 863-868 (2005). https://doi.org/10.1039/b504551g
  4. E. Thrush, O. Levi, W. Ha, K. Wang, S. J. Smith, and J. S. Harris, "Integrated bio-fluorescence sensor," J. Chromatogr. A 1013, 103-110 (2003). https://doi.org/10.1016/S0021-9673(03)01361-X
  5. R. Gihan, H. Jingsong, H. Oliver, A. W. Claire, Y. Y. Jasmine, D. Gareth, M. Alan, J. Simon, C. John, J. Andrew, D. C. Donal, and T. Vo-Dinh, "Highly sensitive fluorescence detection system for Microfluidic lab-on-a-chip," Lab Chip 11, 1664-1670 (2011). https://doi.org/10.1039/c0lc00586j
  6. A. Pais, A. Banrjee, D. Klotzkin, and I. Papautsky, "Highsensitivity, disposable lab-on-a-chip with thin-film organic electronics for fluorescence detection," Lab Chip 8, 794-800 (2008). https://doi.org/10.1039/b715143h
  7. L. Novak, P. Neuzil, J. Pipper, Y. Zhang, and S. Lee, "An integrated fluorescence detection system for lab-on-a-chip application," Lab Chip 7, 27-29 (2007). https://doi.org/10.1039/b611745g
  8. M. L. Chabinyc, D. T. Chiu, J. C. McDonald, A. D. Stroock, J. F. Christian, A. M. Karger, and G. M. Whitesides, "An integrated fluorescencedetection system in poly(dimethylsiloxane) for microfluidic applications," Anal. Chem. 73, 4491-4498 (2001). https://doi.org/10.1021/ac010423z
  9. J. A. Chediak, Z. S. Luo, J. G. Seo, N. Cheung, L. P. Lee, and T. D. Sands, "Heterogeneous integration of CdS filters with GaN LEDs for fluorescence detection microsystems," Sens. Actuators A Phys. 111, 1-7 (2004). https://doi.org/10.1016/j.sna.2003.10.015
  10. J. Choi, K. Kim, and D. Kim, "In situ fluorescence optical detection using a digital micromirror device (DMD) for 3D cell-based assays," J. Opt. Soc. Korea 16, 42-46 (2012). https://doi.org/10.3807/JOSK.2012.16.1.042
  11. R. Walczak, "Fluorescence detection by miniaturized instrumentation based on non-cooled CCD minicamera and dedicated for lab-on-a-chip applications," BioChip J. 5, 271-279 (2011). https://doi.org/10.1007/s13206-011-5312-z
  12. J. Steigert, M. Grumann, T. Brenner, L. Riegger, J. Harter, R. Zengerle, and J. Ducree, "Fully integrated whole blood testing by real-time absorption measurement on a centrifugal platform," Lab Chip 6, 1040-1044 (2006). https://doi.org/10.1039/b607051p
  13. B. R. Schudel, C. J. Choi, B. T. Cunningham, and P. J. A. Kenis, "Microfluidic chip for combinatorial mixing and screening of assays," Lab Chip 9, 1676-1680 (2009). https://doi.org/10.1039/b901999e

피인용 문헌

  1. Recent Advances in Fluorescence Lifetime Analytical Microsystems: Contact Optics and CMOS Time-Resolved Electronics vol.17, pp.12, 2017, https://doi.org/10.3390/s17122800