DOI QR코드

DOI QR Code

붉은장목수수 추출물의 생리활성

Biological Activity of Sorghum bicolor M. cv. Bulgeunjangmoksusu Extracts

  • 김주석 (강원대학교 식물자원응용공학부) ;
  • 이예지 (강원대학교 식물자원응용공학부) ;
  • 양금봉 (강원대학교 식물자원응용공학부) ;
  • 사여진 (강원대학교 식물자원응용공학부) ;
  • 김명옥 (강원대학교 식물자원응용공학부) ;
  • 박종혁 (강원대학교 한방Bio연구소) ;
  • 박동식 (농촌진흥청 국립농업과학원) ;
  • 유창연 (강원대학교 식물자원응용공학부) ;
  • 김명조 (강원대학교 식물자원응용공학부)
  • Kim, Joo-Seok (Division of Bio-resources Technology, Kangwon National University) ;
  • Lee, Yea-Ji (Division of Bio-resources Technology, Kangwon National University) ;
  • Yang, Jinfeng (Division of Bio-resources Technology, Kangwon National University) ;
  • Sa, Yeo-Jin (Division of Bio-resources Technology, Kangwon National University) ;
  • Kim, Myeong-Ok (Division of Bio-resources Technology, Kangwon National University) ;
  • Park, Jong-Hyuk (Oriental Bio-herb Research Institute, Kangwon National University) ;
  • Park, Dong-Sik (Funtional Food & Nutrition Division, Rural Development Administration) ;
  • Yu, Chang-Yeon (Division of Bio-resources Technology, Kangwon National University) ;
  • Kim, Myong-Jo (Division of Bio-resources Technology, Kangwon National University)
  • 투고 : 2012.11.09
  • 심사 : 2013.02.12
  • 발행 : 2013.02.28

초록

본 연구에서는 붉은장목수수(Sorglum Bicolor M.) MeOH 추출물과 분획물들의 다양한 생리활성을 비교하기 위하여 항산화(DPPH assay, 환원력, 총 페놀 및 플라보노이드 함량), 항당뇨(${\alpha}$-Glucosidase 저해활성 및 ${\alpha}$-Amlyase 저해활성), 항암(MTT assay) 활성을 관찰 하였다. 항산화활성의 경우 EtOAc fraction이 높은 총 페놀과 플라보노이드 함량을 나타내었으며, DPPH assay와 reducing power 결과 역시 EtOAc fraction과 MeOH fraction이 positive control로 사용한 ${\alpha}$-tocopherol보다 우수한 활성을 나타냈다. ${\alpha}$-Glucosidase, ${\alpha}$-amlyase 저해능 평가 결과 D.W. fraction이 가장 높은 활성을 보였으며 n-BuOH fraction과 MeOH extract도 활성을 나타내었다. 암세포인 AGS, HT29, HCT116세포주에 대한 세포독성 연구인 MTT assay에서는 EtOAc, n-BuOH, D.W. fraction을 처리한 처리구에서 독성을 보였으며 이중 n-BuOH fraction이 모든 세포주에서 농도 의존적으로 세포독성을 가지는 것을 확인할 수 있었다. 연구결과 붉은장목수수의 추출물 EtOAc fraction의 항산화활성, D.W. fraction의 항당뇨활성, n-BuOH fraction의 암세포에 대한 독성과 관련된 화합물의 분리 및 구조규명에 관한 연구가 필요 할 것으로 사료되어지며, normal cell에 대한 MTT assay를 진행하여 항암활성에 관한 연구를 진행하여 붉은장목수수 추출물을 이용한 다양한 건강기능식품과 의약품 개발을 통해 건강 증진과 질병으로부터 보호 받을 수 있을 것으로 기대한다.

The objective of this study was to determine the biological activities of Sorglum bicolor extracts. Organic fractions, including n-Hexane, EtOAc, and n-BuOH fractions were obtained from the methanol extract of Sorglum bicolor M.. In DPPH radical scavenging activity, $SC_{50}$ values of methanol extract and EtOAc fraction were exhibited $0.66{\pm}0.26{\mu}g/mL$ and $1.03{\pm}0.02{\mu}g/mL$, respectively. Contents of total polyphenol and flavonoids in EtOAc fraction, which were much higher than those of other fractions, were 58.12 mg/g and 4.79 mg/g respectively. Also, effects of reducing power was strongly showed in EtOAc fraction. ${\alpha}$-Glucosidase and ${\alpha}$-amlyase inhibition activities were showed the higher effect in D.W. fraction ($2.83{\mu}g/mL$, $36.64{\mu}g/mL$). In MTT assay in the AGS, HT29 and HCT116 cell lines were significantly higher in the n-BuOH fraction than in the other fractions at $50{\mu}g/mL$ concentration of extracts.

키워드

참고문헌

  1. Awika, J.M., L. Yang, J.D. Browning and A. Faraj. 2009. Comparative antioxidant, antiproliferative and phase II enzyme inducing potential of sorghum (Sorghum bicolor) varieties. Food Sci. Technol. 42:1041-1046.
  2. Blois, M.S. 1955. Antioxidant determination by the use of a stable free radical. Nature 181:165.
  3. Chae, K.Y. and J.S. Hong. 2006. Quality characteristics of sulgidduk with different amounts of waxy sorghum flour. Korean J. Food Cookery Sci. 22:363-369.
  4. Choi, Y.H., M.Y. Kang and S.H. Nam. 1998. Inhibition effect of various cereal and bean extracts on carcinogenicity in vitro. Korean J. Food Sci. Technol. 30:964-969.
  5. Kim, H.S. and Y.H. Kang. 2010. Antioxidant activity of ethanol extracts of non-edible parts (stalk, stem, leaf, seed) from oriental melon. Korean J. Plant Res. 23(5):451-457.
  6. Kim, K.O., H.S. Kim and H.S. Ryu. 2006. Effect of Sorghum bicolor L. Moench(sorghum, su-su) water extracts on mouse immune cell activation. J. Korean Diet. Assoc. 12:82-88.
  7. Kwak, C.S., S.J. Lim, S.A. Kim, S.C. Park and M.S. Lee. 2004. Antioxidative and antimutagenic effects of Korean buckwheat, sorghum, millet, and job's tears. J. Korean Soc. Food Sci. Nutr. 33:921-929. https://doi.org/10.3746/jkfn.2004.33.6.921
  8. Moreno, M.I.N., M.I. Isla, A.R. Sampietro and M.A. Vattuone. 2000. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J. Ethnopharmacology. 71(1-2):109-114. https://doi.org/10.1016/S0378-8741(99)00189-0
  9. Oyaizu, M. 1986. Studies on products of browning reactions: antioxidative activities of products of browning reaction prepared from glucosamine. Jap. J. Nutr. 44:307-315. https://doi.org/10.5264/eiyogakuzashi.44.307
  10. Ryu, H.S., J. Kim and H.S. Kim. 2006. Enhancing effect of Sorghum bicolor L. Moench (sorghum, su su) extracts on mouse spleen and macrophage cell activation. J. Korean Diet. Assoc. 19:176-182.
  11. Sa, Y.J., J.S. Kim, M.O. Kim, H.J. Jeong, C.Y. Yu, D.S. Park and M.J. Kim. 2010. Comparative study of electron donating ability, reducing power, antimicrobial activity and Inhibition of $\alpha$-glucosidase by Sorghum bicolor extracts. Korean J. Food Sci. Technol. 42:598-604.
  12. Taga, M.S., E.E. Miller and D.E. Pratt. 1984. Chia seeds as a source of natural lipid antioxidants. J. Am. Oil Chem. Soc. 61:928-931. https://doi.org/10.1007/BF02542169
  13. Thannickal, V.J. and B.L. Fanburg. 2000. Reactive oxygen species in cell signaling. Am. J. Physiol. Lung Cell Mol. Physiol. 279:L1005-L1029.
  14. Yang, Y.J., H.J. Kim, S.H. Kang and S.C. Kang. 2011. Screening of natural herb resources for anti-oxidative effects in Korea. Korean J. Plant Res. 24:1-9 (in Korean). https://doi.org/10.7732/kjpr.2011.24.1.001

피인용 문헌

  1. Evaluation of antioxidant, α-glucosidase inhibition and acetylcholinesteraseinhibition activities of Allium hookeri root grown in Korea and Myanmar vol.23, pp.2, 2016, https://doi.org/10.11002/kjfp.2016.23.2.239
  2. Antioxidant and Antiproliferative Activity of Extracts from Water Chestnut (Trapa japonica Flerow) vol.24, pp.1, 2016, https://doi.org/10.7783/KJMCS.2016.24.1.14
  3. A Comparative Study on the Physiological Activities of Puffed Snack using Miscellaneous Cereals and Grain Crops vol.27, pp.5, 2014, https://doi.org/10.9799/ksfan.2014.27.5.962
  4. 그라비올라 잎 추출물의 이화학적 특성 및 항산화 활성 vol.28, pp.5, 2013, https://doi.org/10.5352/jls.2018.28.5.540