Journal of Sericultural and Entomological Science (한국잠사곤충학회지)

Korean Society of Sericultural Science (한국잠사학회)
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Quantitative Analysis of 1-deoxynojirimycin in Mulberry Leaves

뽕나무 유전자원의 1-deoxynojirimycin 함량 변이

  • Published : 2013.04.30
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Abstract

Countries of the world are competing for the collection and utilization of genetic resources, which is a resource of value-added industrialization. We are building database of silkworm and mulberry resources with morphological, genetic characteristics data etc. Moreover, recent bioactive compound has been receiving increasing attention. So we analyzed 1-deoxynojirimycin(1-DNJ) content as a this item in addition to the basic information using 363 strains(varieties) of mulberry genetic resources. They were grown under the same environment and conditions. Mulberry leaves were collected and then freeze-dried and powdered for 1-DNJ test. As a result, 1-DNJ mean content of 363 mulberry strains was $0.176{\pm}0.077%$, and the coefficient of variation (CV) was 43.5%. The variation between the strains was greatly severe. Among the tested strains, 'C1D89/29' was showed the highest content of 0.47%, whereas 'Pumbo 24' and 'Turkey E' were showed the lowest content of 0.05% respectively. The content of 1-DNJ of 16 mulberry varieties for silkworm rearing were compared. Average concentration was $0.17{\pm}0.04%$, and the coefficient of variation (CV) was 22.8%. Variation among cultivars was not significant. Finally we selected 1-DNJ high-containing 11 strains. They are as follows. 'C1D89/29', 'Hiihak', 'Jeokasibmunja', 'Gweonchil', 'Botongsibmunja', 'Jeokchuk', 'bulguksang', 'Geunsookgojo', 'Busanggeum', 'Guksang 20' and 'Taekwang' which are more than twice of the overall average content.

세계 각국은 유전자원의 확보 및 고부가 산업화 자원으로 이용하기 위해 경쟁하고 있다. 현재 잠상 유전자원에 대한 가치와 활용도를 높이기 위해 형태적, 유전적 특성을 분석평가하여 데이터베이스를 구축 중에 있으며, 기능성 성분이나 효능에 대한 소비자들의 관심이 높아짐에 따라 기초정보 외에 이들의 항목에 대한 정보를 추가할 필요성이 제기되었다. 따라서 우리나라 620계통(품종)의 뽕나무 유전자원 중 재배환경 및 재배법이 동일한 363계통의 춘기 5개엽기 뽕잎을 채취한 후 동결건조 및 분말로 제조하여 뽕나무 유전자원의 계통별 1-DNJ 함량을 분석하였다. 그 결과, 뽕나무 유전자원 363계통의 1-DNJ 평균함량은 $0.176{\pm}0.077%$이었으며, 변이계수(CV)는 43.5%로서 계통간 변이가 매우 심한 것으로 나타났다. 이 중 'C1D89/29'은 0.47%로서 가장 높았으며, '품보24', '터키 E'은 각각 0.05%로 가장 낮은 함량을 나타냈다. 뽕나무 유전자원 중 누에사육용 16품종의 1-DNJ 함량을 비교하였다. 평균 함량은 $0.17{\pm}0.04%$이었으며, 변이계수(CV)는 22.8%로서 계통간 변이가 크지 않았다. 한편 뽕나무 유전자원 중 1-DNJ 고함유 계통을 선발하였다. 'C1D89/29', '희학', '적아십문자', '권칠', '보통십문자', '적축', '불국상', '근소옥고조', '부상금', '국상20호' 및 '태광' 11계통으로서 전체 평균 함량의 2배 이상 되는 계통들이다.

Keywords

References

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