The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology (한방안이비인후피부과학회지)

The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology (대한한방안이비인후피부과학회)
권호 표지
DOI QR코드

DOI QR Code

Inhibitory Effects of Methanol Extract of Kaempferia galanga on melanogenesis in B16/F10 Melanoma Cells

B16/F10 흑색종양세포에서 삼내자 메탄올 추출물의 멜라닌 생성에 미치는 억제효과

  • Yoon, Jung-Won (Dept. of Oriental Medical Opthalmology & Otolaryngology & Dermatology, Dong-eui University) ;
  • Han, Jung-Min (Dept. of Oriental Medical Opthalmology & Otolaryngology & Dermatology, Dong-eui University) ;
  • Yoon, Hwa-Jung (Dept. of Oriental Medical Opthalmology & Otolaryngology & Dermatology, Dong-eui University) ;
  • Ko, Woo-Shin (Dept. of Oriental Medical Opthalmology & Otolaryngology & Dermatology, Dong-eui University)
  • 윤정원 (동의대학교 한의과대학 안이비인후피부과학교실) ;
  • 한정민 (동의대학교 한의과대학 안이비인후피부과학교실) ;
  • 윤화정 (동의대학교 한의과대학 안이비인후피부과학교실) ;
  • 고우신 (동의대학교 한의과대학 안이비인후피부과학교실)
  • Received : 2013.01.11
  • Accepted : 2013.02.15
  • Published : 2013.02.25
Download PDF
⟨ Previous Next ⟩

Abstract

Objective: Recently the demands for the effective and safe depigmentative and anti-aging agents of the skin have increased due to the medical, pharmaceutical and cosmetic reasons. The purpose of this study is to investigate the MKG(Methanol Extract of Kaempferia galanga) and their dermal bioactivity properties related to cosmeceuticals such as depigmentation. Methods: We assessed inhibitory effects of MKG on melanin production in B16/F10 melanoma cells, on mushroom tyrosinase activity, effects of MKG on the expression tyrosinase, TRP-1, TRP-2, GSK-$3{\beta}$, CREB, MITF in B16/F10 melanoma cells without cytotoxicity range. Cell viability was measured by MTT assay and tyrosinase activity was assessed using by DOPA staining, western-blot analysis. We measured inhibition of melanin synthesis and tyrosinase activity by down-regulation of melanogenic enzyme expressions in ${\alpha}$-MSH induced melanogenesis B16/F10 melanoma cells. Results: MKG inhibited tyrosinase-activity, total melanin contents and dendrite out-growth. MKG inhibited melanogenesis by down-regulation of tyorsinase, TRP-1, TRP-2, CREB, and MITF in B16/F10 cells. The treatment with MKG at the 12.5, $25{\mu}g/ml$ level significantly inhibited the melanin synthesis induced ${\alpha}$-MSH in B16/F10 melanoma cells compared with untreated control. Conclusion: These results suggest that MKG inhibit melanin biosynthesis which is involved in hyper-pigmentation. So MKG is considered to be used as a whitening components reducing cytotoxicity.

Keywords

References

  1. Lee SH, Lee SE, Ahn SG, Hong SP. Skin Barrier. Seoul:Yeomoongak. 2010.
  2. Chenm JS, Wei C, Marshall MR. Inhibition mechannism of Kojic acid on polyphenol oxidase. J Agric Food Chem. 1991;39:1897-901. https://doi.org/10.1021/jf00011a001
  3. Urabe K, Aroca P, Tsukamoto K, Mascagna D, Paulumbo A, Prota G, et al. The inherent cytotoxicty of melanin precursors. Biochim Biophys Acta. 1994;1221:272-8. https://doi.org/10.1016/0167-4889(94)90250-X
  4. Tsukamoto K, Jackson IJ, Urabe K, Montague PM, Hearing VJ. A second tyrosinase-related protein, TRP-2, is a melanogenic enzyme termed DOPAchrome tautomerase. EMBO J. 1992;11(2):519-26.
  5. Boissy RE, Sakai C, Zhao H, Kobayashi T, Hearing VJ. Human tyrosinase related pretein-1(TRP-1) does not funcion as a DHICA oxidase acivity in contrast to murine TRP-1. Exp Dermatol. 1998;7(4):198-204. https://doi.org/10.1111/j.1600-0625.1998.tb00324.x
  6. Curto EV, Kwong C, Hermersdörfer H, Glatt H, Santis C, Virador V, et al. Inhibitors of mammalian melanocytes tyrosinase: In vitro comparisons of alkyl esters of Gentisic acid with other putative inhibitors. Biochemical Pharmacology. 1992;7:663-72.
  7. Chakraborty AK, Funasaka Y, Komoto M, Ichihashi M. Effect of arbutin on malanogenic proteins in human melanocytes. Pagment Cell Res. 1998;11(4):206-12. https://doi.org/10.1111/j.1600-0749.1998.tb00731.x
  8. Park JS, Nam WY, Moon YJ, Cho KH, Jeon BH, Woo WH. Effect of Seosiokyongsan on the Melanogenesis of B16 Melanoma Cell Line. Korean J oriental medical pathology. 2000;14(1):160-70.
  9. Woo WH, Nam WY, Jeong WY, Park RG, Kim JS, Kim WS, et al. Effect of Seosiokyoungsan on the Signaling Pathway of Melanogenesis. Korean journal of oriental medical pathology. 2001;15(1):73-83.
  10. JM Calderon-Montano, E Burgos-Moron, C Perez-Guerrero, M. Lopez-Lazaro. A Review on the Dietary Flavonoid Kaempferol. Medicinal Chemistry. 2011;11:298-344.
  11. Jang DI, Kim MJ, Shin ES, Lee JS. Cosmetic compositions for skin whitening containingextract of galenical medicines. Korean intellectual property office. Patent application:1019990045952. 1999.
  12. Moon KT, Choi JH, Ahn JH, Ha WH, Kim YH, Roh SS. Tooth whitening composition using oriental drug. Korean intellectual property office. Patent application:1020030003341. 2003.
  13. Lee NH. Beauty treatment soap made of natural material. Korean intellectual property office. Patent application:1019870011783. 1987.
  14. Ridtitid W, Sae-wong C, Reanmongkol W, Wongnawa M. Antinociceptive activity of the methanolic extract of Kaempferia galanga Linn. in experimental animals. J Ethnopharmacol. 2008;118:225-30. https://doi.org/10.1016/j.jep.2008.04.002
  15. Sulaiman MR, Zakaria ZA, Daud IA, Ng FN, Ng YC, Hidayat MT. Antinociceptive and anti-inflammatory activities of the aqueous extract of Kaempferia galanga leaves in animal models. Nat Med. 2008;62:221-7. https://doi.org/10.1007/s11418-007-0210-3
  16. Tara Shanbhag B, Chandrakala S, Sachidananda A, Kurady BL, Smita S, Ganesh S. Wound healing activity of alcoholic extract of Kaempferia galanga in Wistar rats. Indian Physiol Pharmacol. 2006;50:384-90.
  17. Mosmann T. Rapid colorimetric assay for the cellular growth and survival; application to proliferation and cytotoxic assay. J Immun Methods. 1983;65:55-63. https://doi.org/10.1016/0022-1759(83)90303-4
  18. MartinezEsparza M, Jimenez-Cervantes C, Solano F, Lozano JA, GarciaBorron JC. Mechanism of melanogenesis inhibition by tumor necrosis factoralpha in B16/F10 mouse melanoma cells. Eur J Biochem. 1998;255:139-46. https://doi.org/10.1046/j.1432-1327.1998.2550139.x
  19. Hosoi J, Abe E, Suda T, Kuroki T. Regulation of melanin synthesis of B16 mouse melanoma cells by 1${\alpha}$, 25dehydroxyvitamin D3 and retinoic acid. Cancer Res. 1985;45:1474-8.
  20. Jeongugimsangbyeongnigyosuhyeobuihoe. Histotechnology. Seoul:Goryeouihak. 1998.
  21. Provance DW, Jr Wei M, Ipe V, Mercer JA. Cultured melanocytes from dilute mutant mice exhibit dendritic morphology and altered melanosome distribution. Proc Natl Acad Sci USA. 1996;93:14554-8. https://doi.org/10.1073/pnas.93.25.14554
  22. Kim DS, Park SH, Kwon SB, Youn SW, Park KC. Effects of lysophosphatidic acid on melanogenesis. Chem and Phy of Lipid. 2004;127:199-206. https://doi.org/10.1016/j.chemphyslip.2003.11.002
  23. Hearing VJ. Biochemical control of melanogenesis and melanosomal organization. J Investig Dermatol Symp Proc. 1999;4(1): 24-8. https://doi.org/10.1038/sj.jidsp.5640176
  24. Eun HC, et al. Skin immunology. Seoul:Soeul university press. 1999:143.
  25. K Jimbow, H Chen, JS Park, PD Thomas. Increased sensitivity of melanocytes to oxidative stress and abnormal expression of tyrosinase-related protein in viltigo. British J Derma. 2001;144:55. https://doi.org/10.1046/j.1365-2133.2001.03952.x
  26. Kim EC, Ahn SY, Hong ES, Lee GH, Kim EK, Row KH. Extraction of whitening agents from natural plants and whitening effect. J Korean Ind Eng Chem. 2005;16(3):348-53.
  27. Ha BJ. Functional cosmetics. Seoul:Singwang. 2001:66-84,138-40,143-7.
  28. Moon YJ, Kim J, Yim NY, Lee SY, Kwak S, Hwang CY, et al. Inhibitory Effect on Melanogenesis of Radix Glycyrrhizae Water Extract. Korean journal of oriental physiology & pathology. 2002;16(6):1230-5.
  29. Chun HJ, Choi EY, Yoon SC, Nam HW, Beak SH. Inhibitory Effects of Ethanol Extract of Atractylodis Rhizoma alba on Melanin Biosynthesis. Yakakoeji. 2001;45(3): 269-75.
  30. Lee GS, Kim JJ, Song CS, Oh CG, Yim GS. Effect of Radix Trichosanthis on the Melanogenesis. The Journal of Oriental Medical Surgery, Ophthalmology & Otolaryngology. 2001;14(1):209-25.
  31. Lee SY, Kim JM, Oh HC, Yim SJ, Hwang CY, Moon YJ, et al. The effect of Codonopsis lanceolata on the melanogenesis. The Korean journal of oriental medical prescription. 2002;10(2):199-211.
  32. Yim NY, Kwon GJ, Kim YS, Beak SG, Yim JR, Moon YJ, et al. Inhibitory Effect of Methanolic Extract from Adenophorae Radix on Melanogenesis. Korean journal of oriental physiology & pathology. 2004;18(3):747-53.
  33. Yoon HJ, Yoon JW, Yoon SW, Ko WS, Woo WH. Inhibitory effect on melanogenesis of Rhizoma Bletillae. The Journal of Oriental Medical Ophthamology & Otolaryngology & Dermatology. 2003;16(1):100-11.
  34. Oh HC, Yim GS, Hwang CY, Yoon IH, Kim NG. A Study on the Melanin Synthesis Inhibition and Whitening Effect of Bombysis Corpus. The journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology. 2007;20(3):1-13.
  35. Kim CH, Jung HA, Noh SS, Hong SH. Effects of Aloe and Violae herba Extract on the Anti-oxidant, Anti-inflammatory, Anti-wrinkle and Whitening. The journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology. 2010;23(1):23-43.
  36. Park JM, Kim KJ. The Anti-wrinkle Effects and Whitening Effects of Galla Rhois. The journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology. 2010;23(1):135-48.
  37. Hwangbo M, Noh SS, Soe HS. Effects of Dendrobii herba and Punica granatum Extract on the Anti-oxidant, Anti-inflammatory, Anti-wrinkle and Whitening. The journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology. 2010;23(3): 11-32.
  38. Kim DS, Sung BG, Lee JC, Lee BG, Woo WH, Yim GS. Inhibitory Effect of Belamcandae Rhizoma on the Melanogenesis in MSH-stimulated B16F10 cells. The journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology. 2011;24(1): 25-35.
  39. Lee SH. Research on Whitening Effect of Ephedra Sinica and Mapunggo. Kyunghee University. 2001.
  40. Lee SE, Kim HJ, Kim Yb. The Study on Depigmentation of Kamibangpungtongsung-San. The journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology. 2004;17(1):94-103.
  41. Park HS, Kim HJ, Kim YB. The Study on Depigmentation of Kamikwibi-Tang. The Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology. 2004;17(2):48-58.
  42. Kim SM, Yoo DY. The Effect of Mibaeksan(MB) on Melanin Synthesis and Gene Expression. The Journal of oriental obstetrics & gynecology. 2009;22(4):1-8.
  43. Kim JK, Yoo DY. The Effect of Yukmijihwangtang -gagambang (YMG) on Melanin Synthesis and Gene Expression. The Journal of oriental obstetrics & gynecology. 2009;22(3):66-82.
  44. Yang YG. Hwangjenaegyeongsomun. Daebuk: Nakgunchulpansaeobyuhangongsa. 1994:624-79.
  45. So WB. Sossijebyeongwonhuron. Seoul: Daeseongmunhwasa. 1992:200.
  46. Shin YS, Roh SS. A Literature studies on the causes and treatments of the melasma. The Journal of Oriental Medical Surgery, Ophthalmology & Otolaryngology. 1998;11 (1):82-98.
  47. Jo G. Seongjechongnok. Beijing:Inminwisaengchulpansa. 1987:1763.
  48. Go SJ. Yanguidaejeon. Beijing:Inminwisaengchulpansa. 1986:479,481-2.
  49. Lee C. Pyeonjuuihagimmun. Seoul:Daeseongmunhwasa. 1990:29,224.
  50. Jin SG. Oegwajeongjong. Shanghai:Sanghaegwahakgisulchulpansa. 1989:290-8.
  51. Ju JH. Dangyeuijip. Beijing:Inminwisaengchulpansa. 1993:24.
  52. Song JS. Hanbangpibumiyong. Hyorim. 1993: 188-92,223-5.
  53. Yang SJ. Junguiimsangdaejeon. China:Bukgyeonggwahakgisulchulpansa. 1991:896-7,923-4.
  54. Heo J. Donguibogam. Seoul:Daeseongmunhwasa. 1985:97-9.
  55. Hwang DY. Daeyeokjeungmaek bangyakhappyeon. Seoul:Namsandang. 1992:282-3.
  56. Seoul Nat'l Univ. cheonyeonmulgwahagyeonguso munheonjeongbohagyeongusilpyeon. Dongyanguiyakgwahakdaejeon. 2003:217.
  57. Jeontonguihagyeonguso. Bonchoyakjaedogam. Seoul:seongbosa. 1994:200.
  58. Go HJ. Whitening Effects of Extract of Kaempferia galanga. Chungang Univ. 2011.
  59. Bae IW. Study on compounds having strong inhibition effect toward tyrosinase and glutathione S-tranferase activities from natural products. Chungang Univ. 2005.
  60. Wu D, Pan W. GSK3: a multifaceted kinase in Wnt signaling. Trends Biochem Sci. 2010;35(3):161-8. https://doi.org/10.1016/j.tibs.2009.10.002
  61. Bienz M. beta-Catenin: a pivot between cell adhesion and Wnt signalling. Curr Biol. 2005;15(2):64-7.
  62. Leeuw SM, Smit NP, Van Veldhoven M, Pennings EM, Pavel S, Simons JW, et al. Melanin content of cultured human melanocytes and UV-induced cytotoxicity. J Photochem Photobiol B. 2001;61(3):106-13. https://doi.org/10.1016/S1011-1344(01)00168-3
  63. Lerner AB, McGuire JS. Effect of alpha- and beta-melanocyte stimulating hormone on the skin color of man. Nature. 1961.
  64. Hunt G, donatien PD, Lunec J. Cultured human melanocytes respond to MSH peptides and ACTH. Pigment Cell Res. 1994;7:217-21. https://doi.org/10.1111/j.1600-0749.1994.tb00052.x
  65. Busca R, Bertolotto C, Abbe P, Englaro W, Ishizaki T, Narumiya S, et al. Inhibition of Rho is required for cAMP-induced melanoma cell differentiation. Mol Biol Cell. 1998;9:1367-78. https://doi.org/10.1091/mbc.9.6.1367

Cited by

  1. Leathesia difformis Extract Inhibits α-MSH-Induced Melanogenesis in B16F10 Cells via Down-Regulation of CREB Signaling Pathway vol.20, pp.3, 2019, https://doi.org/10.3390/ijms20030536