Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
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The expression of a nitric oxide derivative, tissue inhibitors of metalloproteinase-3, and tissue inhibitors of metalloproteinase-4 in chronic periodontitis with type 2 diabetes mellitus

  • Jung, Hyun-Yub (Department of Periodontology, Kyungpook National University School of Dentistry) ;
  • Kim, Yong-Gun (Department of Periodontology, Kyungpook National University School of Dentistry) ;
  • Park, Jin-Woo (Department of Periodontology, Kyungpook National University School of Dentistry) ;
  • Suh, Jo-Young (Department of Periodontology, Kyungpook National University School of Dentistry) ;
  • Lee, Jae-Mok (Department of Periodontology, Kyungpook National University School of Dentistry)
  • Received : 2012.12.20
  • Accepted : 2013.03.10
  • Published : 2013.04.30
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Abstract

Purpose: The purpose of this study was to analyze the expression of inducible nitric oxide synthases (iNOS), tissue inhibitors of metalloproteinase $(TIMP)_{-3}$, and $TIMP_{-4}$ in the gingival tissues of periodontal patients with or without type 2 diabetes mellitus (DM). Methods: Depending on the patient's systemic condition and clinical criteria of the gingiva, each gingival sample was classified into one of three groups. Sixteen clinically, systemically healthy patients (group 1), 16 periodontal patients (group 2), and 16 periodontal patients with DM (group 3) were included. Tissue samples in each group were collected, prepared, and analyzed by western blotting. Quantification of the relative amount of $TIMP_{-3}$, $TIMP_{-4}$, and iNOS was performed. Results: The expression levels of iNOS and $TIMP_{-3}$ both increased in group 1, group 2, and group 3 in increasing order, and were significantly higher in both group 2 and group 3 as compared to group 1 (P<0.05). The expression levels of $TIMP_{-4}$ increased in the same order, but significantly increased in group 2 as compared to group 1, in group 3 as compared to group 1, and group 3 as compared to group 2 (P<0.05). Conclusions: This study demonstrated that iNOS, $TIMP_{-3}$, and $TIMP_{-4}$ might be involved in the progression of periodontal inflammation associated with type 2 DM. It is thought that further study of these factors can be applied practically for the diagnosis and control of periodontitis in diabetics.

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References

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