Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
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Antioxidant and tyrosinase inhibitory activity of Hypsizygus marmoreus (brown cultivar) methanol extracts

갈색 느티만가닥버섯 메탄올 추출물의 항산화 및 tyrosinase 저해 효과

  • Kim, Su Cheol (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology) ;
  • Ryu, Han Min (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology) ;
  • Jung, Sung Mi (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology) ;
  • Lee, Young Hyun (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology) ;
  • Kim, Hye Soo (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology) ;
  • Kim, Jong Ok (Namkyung Co., Ltd.) ;
  • Cho, Young Un (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology) ;
  • Cho, Soo Jeong (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology)
  • 김수철 (경남과학기술대학교 제약공학과) ;
  • 류한민 (경남과학기술대학교 제약공학과) ;
  • 정성미 (경남과학기술대학교 제약공학과) ;
  • 이용현 (경남과학기술대학교 제약공학과) ;
  • 김혜수 (경남과학기술대학교 제약공학과) ;
  • 김종옥 (남경농산) ;
  • 조용운 (경남과학기술대학교 제약공학과) ;
  • 조수정 (경남과학기술대학교 제약공학과)
  • Received : 2013.12.11
  • Accepted : 2013.12.27
  • Published : 2013.12.31
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Abstract

The objective of this study was to evaluate antioxidant effect and tyrosinase inhibitory activity of methanol extracts from Hypsizygus marmoreus. The Hypsizygus marmoreus was divided into two parts (pileus and stipe) and extracted with methanol. Total polyphenolics and flavonoids in the methanol extracts were measured by spectrophotometric methods and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities have been determined for antioxidant activities. The total polyphenolics and flavonoids contents of methanol extract of the pileus were higher than methanol extract of the stipes. The total polyphenolics contents in methanol extracts of the pileus and stipes were 8.7 ug/mg and 5.6 ug/mg, respectively. The total flavonoids contents in methanol extracts of the pileus and stipes were 2.8 ug/mg and 1.4 ug/mg, respectively. The tyrosinase inhibitory activity was proportional to concentration of methanol extract. The tyrosinase inhibitory activity of the methanol extract (200 mg/ml) of pileus (66.9%) and stipe (57.97%) was lower than those of positive control 2% arbutin. The DPPH radical scavenging activity of the methanol extract (20 mg/ml) of pileus and stipes was 52.55% and 30.35%, respectively. Moreover, the effects of methanol extarcts on cell proliferation of B16BL6 mouse melanoma cells were investigated using WST-1 assay (4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulphonate) and B16BL6 mouse melanoma cells treated with methanol extract of 200-2,000 ug/ml were higher proliferation rate than those of 0.04% adenosine.

본 연구에서는 기능성 식품 및 화장품 소재로써 갈색 느티만가닥버섯(Hypsizygus marmoreus)의 이용가능성을 조사하기 위해서 갈색 느티만가닥버섯 메탄올 추출물의 항산화 활성 및 tyrosinase 저해 효과를 갓(pileus)과 대(stipes)로 분리하여 부위별로 조사하였다. 메탄올 추출물 중 갓과 대의 총 폴리페놀 함량은 각각 $8.7{\pm}3.27$ ug/mg과 $5.6{\pm}2.85$ ug/mg이었고 플라보노이드 함량은 각각 $2.8{\pm}3.81$ ug/mg과 $1.4{\pm}1.95$ ug/mg이었으며 총 폴리페놀과 플라보노이드 함량 모두 대보다 갓에서 높게 나타났다. Tyrosinase 저해 활성은 추출물의 농도에 따라 증가하는 경향을 나타내었으나 양성 대조구로 사용한 2% 알부틴(arbutin)과 비교했을 때 1200 mg/ml의 고농도에서도 갓은 66.9%, 대는 57.97%의 낮은 저해 활성을 나타내었다. 항산화 활성은 DPPH에 의한 라디칼소거 활성을 측정하여 확인하였으며 갓과 대의 DPPH 라디칼 소거능은 20 mg/ml의 농도에서도 각각 52.55%와 30.35%로 낮게 나타났다. 추출물이 B16BL6 mouse melanoma cell에 미치는 영향을 알아보기 위해 WST-1 assay (4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulphonate)를 이용하여 세포생존율을 조사하였으며 갓과 대 모두 200-2,000 ug/ml의 농도로 처리하였을 때 100% 이상의 생존율을 나타내었고 추출물의 처리 농도가 증가함에 따라 세포생존율도 증가하는 경향을 나타내었으며 양성대조군인 0.04% adenosine을 처리한 경우보다 높게 나타났다.

Keywords

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