한국버섯학회지 (Journal of Mushroom)

  • 제11권3호
  • /
  • Pages.171-176
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  • 2013
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  • 1738-0294(pISSN)
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  • 2288-8853(eISSN)
한국버섯학회 (The Korean Society of Mushroom Science)
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큰느타리버섯의 저온적응성 형질에 관련된 SCAR Marker 개발

Development of a psychrophilic-SCAR marker for Pleurotus eryngii

  • 김수철 (경남과학기술대학교 제약공학과) ;
  • 황혜성 (경남과학기술대학교 제약공학과) ;
  • 조윤진 (경남과학기술대학교 제약공학과) ;
  • 김혜수 (경남과학기술대학교 제약공학과) ;
  • 류재산 (경상남도농업기술원 친환경연구과) ;
  • 조수정 (경남과학기술대학교 제약공학과)
  • Kim, Su Chul (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology) ;
  • Hwang, Hye Sung (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology) ;
  • Cho, Yun Jun (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology) ;
  • Kim, Hye Su (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology) ;
  • Ryu, Jae-San (Eco-friendliness Research Department, Gyeongnam-doAgricultural Rearch and Extension Services) ;
  • Cho, Soo Jeong (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology)
  • 투고 : 2013.07.17
  • 심사 : 2013.09.16
  • 발행 : 2013.09.30
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초록

큰느타리버섯의 저온성적응성 형질에 관련된 SCAR marker를 개발하기 위하여 저온성 계통의 8균주와 대조구 8균주의 genomic DNA를 30 ug/ml의 농도로 bulking한 것을 주형 DNA로 사용하고 operon 사의 OPA(20개), OPB(20개), OPL(20개), OPP(20개), OPR(20개), OPS(20개) 등 총 120개 primer를 random primer(10 mer)로 사용하여 RAPD를 수행하였으며 이중에서 OP-S3 primer를 사용한 PCR 산물들이 대조구와 가장 뚜렷한 차이를 나타내었다. OP-S3 primer를 이용한 RAPD 결과, 약 480 bp 부근에서 저온성 계통에 특이적인 DNA band가 관찰되었으며 이 DNA band의 염기서열을 근거로 SCAR marker로 사용할 specific primer인 OP-S3-1-F와 OP-S3-1-R를 디자인하였다. SCAR marker OP-S3-1 primer를 이용하여 PCR을 수행한 결과에서는 저온성 계통에서만 480 bp 부근에서 대조구와 구별되는 DNA band를 확인할 수 있었으며 random primer인 OP-S3 primer를 이용하여 PCR을 수행했을 때보다 재현성이 높고 진한 DNA band를 확인할 수 있었다.

Genomic DNAs of psychrophilic strains of Pleurotus eryngii were analyzed by randomly amplified polymorphic DNA (RAPD) using OP-A, OP-B, OP-L, OP-P, OP-R and OP-S3 primers to develop the strain-specific DNA marker. A unique DNA fragment with the size of 480 bp was yielded by OP-S3 primer from the psychrophilic strain. A sequence characterized amplified region (SCAR) marker, designated as OP-S3-1, was designed on the basis of the determined sequence. The PCR analysis with the OP-S3-1 primer showed that this SCAR marker can clearly distinguish the psychrophilic strains from the control strains.

키워드

참고문헌

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피인용 문헌

  1. Development of strain-specific SCAR marker for selection of Pleurotus eryngii strains with higher β-glucan vol.13, pp.1, 2015, https://doi.org/10.14480/JM.2015.13.1.79
  2. Development of strain-specific SCAR marker for selection of Pleurotus eryngii strains adaptable to high-temperature vol.12, pp.3, 2014, https://doi.org/10.14480/JM.2014.12.3.226