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Characteristics and breeding of a new multi-generation oyster mushroom (Pleurotus ostreatus) variety 'Dagul'

다발성 신품종 느타리 '다굴'의 육성 및 자실체 특성

  • Shin, Pyung-Gyun (Mushroom Research Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Kim, Hee-Jung (Mushroom Research Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Choi, Chan-Sik (Mushroom Research Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Yoo, Young-Bok (Mushroom Research Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Kong, Won-Sik (Mushroom Research Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Jang, Kab-Yeul (Mushroom Research Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Oh, Youn-Lee (Mushroom Research Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Cheong, Jong-Chun (Mushroom Research Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Suh, Jang-Sun (Mushroom Research Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Oh, Se Jong (Ganghwa Agricultural Technology Service Center) ;
  • Lee, Keum-Hee (Department of Plant Quarantine, APQR)
  • 신평균 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 김희정 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 최찬식 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 유영복 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 공원식 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 장갑열 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 오연이 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 정종천 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 서장선 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 오세종 (강화군농업기술센터) ;
  • 이금희 (농립축산검역본부 식물검역부 위험관리과)
  • Received : 2013.09.11
  • Accepted : 2013.09.27
  • Published : 2013.09.30

Abstract

To develop a new variety of oyster mushroom(Pleurotus ostreatus), parental strains was selected by the method of Mon-Mon crossing between monokaryotic strains derived from ASI 2596(Suhan No.3) and ASI 2782(Black pileus mutant). The SB-73(ASI 2596-11 x 2782-8) was shown the best cultural characteristics, selected to be a new variety and named as 'Dagul'. The 'Dagul' was formed incompatibility line distinctly in the confrontation growth of parental strains Suhan No.3 and ASI 2782. The optimum temperature for mycelial growth, fruiting body development and pH arrange were $25{\sim}30^{\circ}C$, $14{\sim}17^{\circ}C$ and pH5~8, respectively. Fruiting body production per bottle was about $68.0{\pm}24.1$ g which is almost 115% quantity compared to that of other variety Suhan No.3. And also the stipe is long and individual generation is multiple. Analysis of the genetic characteristics of the new variety 'Dagul' showed different DNA bands as that of the control strains, Suhan No.3 and ASI 2782, when RAPD(Random Amplified Polymorphic DNA) primers URP7 and Rcb1 were used. This new variety 'Dagul' of oyster mushroom is characterized by multiple of individual generation and the stipe is long. We therefore expect that this new strain will increase of the income by cultivation of field.

느타리(Pleurotus ostreatus)의 품종 육성은 ASI 2596(수한3호)와 ASI 2782(흑변이체)와 Mon-Mon 교잡을 통하여 3계통을 선발하였다. 선발된 우량계통 중에서 품질이 가장 우수한 SB-73을 '다굴'로 명명하였다. 주요 특성은 균사생장 적온이 $25^{\circ}C$이나 $30^{\circ}C$에서도 잘 자라며, pH의 범위가 pH5~8까지 넓게 형성되었고, 자실체 발생 최적온도는 $14{\sim}17^{\circ}C$였다. 균사체 배양에서 대선형성유무는 모균주 수한3호와 흑변이체와는 뚜렸한 대선을 형성하였다. 자실체 갓의 색깔은 연회색을 나타내었고, 형태로는 대조품종인 수한3호와 같이 다발형이었다. 자실체 수량은 병당 $68.0{\pm}24.1$로 수한3호의 수량지수를 100으로 보았을 때 115를 나타내었다. 또한 대의 길이는 수한3호보다 길고 개체발생수가 $26.1{\pm}7.5$로 수한3호 $10.5{\pm}2.6$보다 많이 형성되어 다발성을 보여주었다. 2종류의 primer를 이용하여 새로운 품종 '다굴'에 대한 RAPD pattern를 분석한 결과 모균주 수한3호와 같은 pattern를 가지면서 다른 밴드가 존재하였다. 신품종 '다굴'은 개체발생수가 많고 대가 길어 재배방법이 확립되면 농가 소득증대에 기여할 것으로 기대된다.

Keywords

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