Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
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Production of ligninolytic enzymes by Pleurotus ostreatus No. 42 in various culture media

다양한 배지에서 느타리버섯 No. 42균주로부터 리그닌분해효소 생산

  • Ha, Hyo-Cheol (Department of Herbal Food Science, Daegu Haany University)
  • 하효철 (대구한의대학교 한방식품약리학과)
  • Received : 2013.06.05
  • Accepted : 2013.06.20
  • Published : 2013.06.30
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Abstract

When No. 42 strain of Pleurotus ostreatus was cultivated at five different media, MnP and Lac but no LiP activity was detected throughout the culture period in the media. The production of MnP and Lac by No. 42 strain of Pleurotus ostreatus were correlated with wheat bran composition in the medium. In the liquid culture, maximum production of MnP and Lac were observed in the medium contained glucose-peptone- yeast-wheat bran(GPYW). However, in solid medium, maximum production of MnP was observed in wood meal-wheat bran(WMW) medium, but that of Lac was observed in wheat bran(W) medium.

느타리 버섯 No. 42균주를 5가지 배지에서 연구한 결과, 리그닌 분해효소인 Lac와 MnP는 생산되었으나 LiP는 생산되지 않았다. 실험한 본 균주의 경우 리그닌 분해효소인 Lac와 MnP의 생산에 밀기울이 관여하는 것을 확인 할 수 있었다. 액체배지에서는 GPYW에서 Lac(2.4 U/ml)와 MnP(3.6 U/ml)가 최대 생산됨을 알 수 있었으며 고체배지에서는 WMW에서 MnP(4.0 U/ml)가 최대 생산되었으며 W에서는 Lac(11.0 U/ml)가 최대 생산되었다.

Keywords

References

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