KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
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Plantlet Regeneration via Somatic Embryogenesis from Hypocotyls of Common Buckwheat (Fagopyrum esculentum Moench.)

  • Kwon, Soo-Jeong (Dept. of Crop Science, Chungbuk National University) ;
  • Han, Myong-Hae (Dept. of Crop Science, Chungbuk National University) ;
  • Huh, Yoon-Sun (Horticultural Research Division, Chungcheongbuk-do Agricultural Research and Extension Services) ;
  • Roy, Swapan Kumar (Dept. of Crop Science, Chungbuk National University) ;
  • Lee, Chul-Won (Dept. of Crop Science, Chungbuk National University) ;
  • Woo, Sun-Hee (Dept. of Crop Science, Chungbuk National University)
  • Received : 2013.09.13
  • Accepted : 2013.10.29
  • Published : 2013.12.30
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Abstract

Buckwheat sprout is used as vegetable, and also flour for making noodles, and so on. Currently, information about tissue culture in buckwheat is limited and restricted to micro-propagation. We carried out somatic embryogenesis and plant regeneration using hypocotyl segments as explant of the cultivated buckwheat species, Fagopyrum esculentum which differs from existing studies in the growth regulator combinations used. Maximum callus regeneration was induced on MS medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) $2.0mg{\cdot}L^{-1}$, benzyladenine (BA) $1.0mg{\cdot}L^{-1}$ and 3% sucrose. Friable callus was transferred to solidified MS media containing BA ($1.0mg{\cdot}L^{-1}$) with various concentrations of 2,4-dichlorophenoxyacetic acid for the induction of embryogenesis. The optimum concentrations of growth regulators (for regeneration of plantlet) were indole-3-acetic acid ($2.0mg{\cdot}L^{-1}$), Kinetin ($1.0mg{\cdot}L^{-1}$), BA ($1.0mg{\cdot}L^{-1}$). Only 2,4-D did not show any significant effect on callus induction or embryogenesis. Regeneration of embryonic callus varied from 5% to 20%. Whole plants were obtained at high frequencies when the embryogenic calli with somatic embryos and organized shoot primordia were transferred to MS media with 3% sucrose. The main objective of this research was to develop an efficient protocol for plant regeneration for common buckwheat, and to apply in future for genetic transformation.

Keywords

References

  1. Adaci, T., S. Suputtitada, and Y. Miike. 1988. Plant regeneration from anther cultures in common buckwheat (Fagopyrum esculentum). Fagopyrum 8 : 5-9.
  2. Adachi, T., A. Yamaguchi, Y. Miike, and F. Hoffmann. 1989. Plant regeneration from protoplasts of common buckwheat (Fagopyrum esculentum). Plant Cell Rep. 8 : 247-250. https://doi.org/10.1007/BF00778544
  3. Al-khayri, J. M., C. E. Shamblin, R. W. Mcnew, and E. J. Anderson. 1996. Callus induction and plant regeneration of U.S. Rice genotypes as affected by medium constituents. In Vitro Cell Dev. Biol-Plant. 32 : 227-232. https://doi.org/10.1007/BF02822692
  4. Ammirato, P. V. 1983. Embryogenesis, In: Evans, D. A., et al., (eds.), Handbook of Plant Cell Culture, Techniques for Propagation and Breeding, pp. 82-123, New York, Macmillan.
  5. Bohanec, B., M. Neskovic, and R. Vujicic. 1993. Anther culture and androgenic plant regeneration in buckwheat (Fagopyrum esculentum Moench.). Plant Cell Tiss. Organ Cult. 35: 259-266. https://doi.org/10.1007/BF00037279
  6. Dutta, M. N. M., S. Shah, and P. Kumar. 2010. Assessment of agro-morphological. Physiological and genetic diversity among buckwheat cultivars. Proc. 11th Intl. Symp. Buckwheat, Orel, Russia, 94-101.
  7. Farzana, A. R. F., P. G. V. N. Palkadapala, K. M. M. N. Meddegoda, P. K. Samarajeewa, and J. P. Eeswara. 2008. Somatic embryogenesis in papaya (Carica papaya L. cv. Rathna). J. Nat. Sci. Found Sri Lanka 36 : 41-50.
  8. Han, M. H., A. H. M. Kamal, Y. S. Huh, A. Y. Jeon, J. S. Bae, K. Y. Chung, M. S. Lee, S. U. Park, H. S. Jeong, and S. H. Woo. 2011. Regeneration of plantlet via somatic embryogenesis from hypocotyls of Tartary Buckwheat (Fagopyrum tataricum). Aus. J. Crop. Sci. 5 : 865-869.
  9. Kohlenbach, H. W. 1978. Comparative somatic embryogenesis. In: Thorpe TA (Ed), Frontiers of Plant Tissue Cultures, Calgary Univ Press, Calgary
  10. Kreft, I. 1983. Breeding breeding perspectives. In: T. Nagatomo, and T. Adachi, (eds.), Buckwheat Research 1983. Proc. 2nd Symp. Buckwheat, Miyazaki, Japan, 113-139.
  11. Lachmann, S. and T. Adachi. 1990. Callus regeneration from hypocotyl protoplasts of tartary buckwheat (Fagopyrum tataricum Gaertn.). Fagopyrum 10 : 62-64.
  12. Lee, Y. K., K. Y. Kim, M. R. Uddin, N. I. Park, and S. U. Park, 2009. An efficient protocol for shoot organogenesis and plant regeneration of buckwheat (Fagopyrum esculentum Moench.). Roman Biotech. Lett. 14:4524-4529.
  13. Miljus-Djukic, J., M. Neskovic, S. Ninkovic, and R. Crkvenjakov. 1992. Agrobacterium-mediated transformation and plant regeneration of buckwheat (Fagopyrum esculentum Moench.). Plant Cell Tiss. Organ Cult. 15 : 473-497.
  14. Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15 : 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  15. Neskovic, M., R. Vujicic, and S. Budimir. 1987. Somatic embryogenesis and bud formation from immature embryos of buckwheat (Fagopyrum esculentum Moench.). Plant Cell Rep. 6 : 423-426.
  16. Neskovic, M., L. Culafic, and R. Vujicic. 1995. Somatic embryogenesis in buckwheat (Fagopyrum Mill.) and sorrel (Rumex L.), Polygonaceae. In: TPS Bajaj, (ed.), Biotechnology in agriculture and forestry, somatic embryogenesis and synthetic seeds II. pp. 412-427, Springer-Verlag, Berlin Heidleberg, New York.
  17. Srejovic, V. and M. Neskovic. 1981. Regeneration of plants from cotyledon fragments of buckwheat (Fagopyrum esculentum Moench.). Z. Pflanzenphysiol. 104 : 37-42. https://doi.org/10.1016/S0044-328X(81)80093-1
  18. Steward, F. C., P. V. Ammirato, and M. O. Mapes. 1970. Growth and development of totipotent cells. some problems, procedures and perspectives. Ann. Bot. 34 : 761-787.
  19. Takahata, T. 1988. Plant regeneration from cultured immature inflorescence of common buckwheat (Fagopyrum esculentum Moench.) and perennial buckwheat (F. cymosum Meisn.). Japan J. Breed. 38 : 409-413. https://doi.org/10.1270/jsbbs1951.38.409
  20. Woo, S. H. and T. Adachi. 1997. Production of interspecific hybrids between Fagopyrum esculentum and F. homotropicum through embryo rescue. SABRAO Genetics J. 29 : 89-95.
  21. Woo, S. H., A. Nair, T. Adachi, and C. Campbell. 2000. Plant regeneration from cotyledon tissues of common buckwheat (Fagopyrum esculentum Moench.). In Vitro Cell. Dev. Biol-Plant, 36 : 358-361. https://doi.org/10.1007/s11627-000-0063-x
  22. Yamane, Y. 1974. Induced differentiation of buckwheat plants from subcultured calluses in vitro. Japan J. Genet. 49 : 139-146. https://doi.org/10.1266/jjg.49.139

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