약학회지 (YAKHAK HOEJI)

  • 제57권6호
  • /
  • Pages.383-387
  • /
  • 2013
  • /
  • 0377-9556(pISSN)
  • /
  • 2383-9457(eISSN)
대한약학회 (The Pharmaceutical Society of Korea)
권호 표지

Obacunone 황백성분의 Candida albicans에 대한 항진균효과

Antifungal Effect of Obacunone on Candida albicans

  • 한용문 (동덕여자대학교 약학대학 면역.미생물학실) ;
  • 김정현 (동덕여자대학교 약학대학 면역.미생물학실)
  • Han, Yongmoon (Department of ImmunoMicrobiology, College of Pharmacy, Dongduk Women's University) ;
  • Kim, Jeonghyeon (Department of ImmunoMicrobiology, College of Pharmacy, Dongduk Women's University)
  • 투고 : 2013.09.23
  • 심사 : 2013.12.02
  • 발행 : 2013.12.31
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초록

In the present study, we determined the antifungal effect of obacunone isolated from Phellodendri Cortex against Candida ablicans, a pathogenic fungus. The antifungal effect was analyzed by an in-vitro susceptibility test and in a murine model of disseminated candidiasis. Possible mechanism of the antifungal activity was also examined. Analyses of data resulting from the susceptibility test revealed that the compound inhibited C. albicans growth. At 25 ${\mu}g$ obacunone/ml, there was app. 45% reduction of CFUs (colony forming units) as compared to obacunone-untreated C. albicans yeast cells (P<0.01). In the murine model of disseminated candidiasis due to C. albicans, obacunone enhanced resistance of mice against disseminated candidiasis. During an entire period of 30-day observation, control animals all died within 14 days, whereas 60% of obacunone-treated mice survived (P<0.05). In addition, obacunone inhibited the hyphal production, a major virulence factor of C. albicans, from the blastoconidial form. Thus, obacunone appears to have antifungal activity for C. albicans infection. This may possibly be mediated by the blockage of hyphal production.

키워드

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