Biomaterials Research (생체재료학회지)

Korean Society for Biomaterials (한국생체재료학회)
권호 표지

Fabrication and Analysis of PLGA Nanofiber with poly-l-lysine for Adhesion and Expansion of Mesenchymal Stem Cell

중간엽줄기세포 부착 및 증식을 위한 poly-l-lysine 도입 PLGA 나노섬유 제조 및 분석

  • Kang, Gwang-Sik (Stem Cell Engineering Laboratory, Department of Biomedical Science, CHA University) ;
  • Cha, Byung-Hyun (Stem Cell Engineering Laboratory, Department of Biomedical Science, CHA University) ;
  • Park, Kwang-Sook (Stem Cell Engineering Laboratory, Department of Biomedical Science, CHA University) ;
  • Kim, Hee-Chun (Department of Orthopedic, CHA University) ;
  • Park, Han-Soo (School of Integrative Engineering, Chung-Ang University) ;
  • Park, Kwi-Deok (Center for Biomaterials, Korea Institute of Science and Technology) ;
  • Lee, Soo-Hong (Stem Cell Engineering Laboratory, Department of Biomedical Science, CHA University)
  • 강광식 (차의과학대학교 의생명과학과) ;
  • 차병현 (차의과학대학교 의생명과학과) ;
  • 박광숙 (차의과학대학교 의생명과학과) ;
  • 김희천 (차의과대학병원 정형외과) ;
  • 박한수 (중앙대학교 융합공학부 바이오메디컬공학과) ;
  • 박귀덕 (한국과학기술연구원 생체재료연구단) ;
  • 이수홍 (차의과학대학교 의생명과학과)
  • Published : 2013.06.01
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Abstract

Cell-substrate interaction is fundamentally essential for phenotypic maintenance and multipotency of stem cells. To regulate the cell-substrate interaction, many researchers have tried to mimic the native cellular environment by introducing physical and chemical factors. Electrospinning technique is a useful tool to produce nanofibers with various diameters, porosities and structures like the native cellular physical environment. In electrospinning technique, however, most synthetic biodegradable polymers such as poly(lactic acid) (PLA), poly(glycolic acid) (PGA) and poly(lactic acid-co-glycolic acid) (PLGA) show low adhesion and proliferation of cells on them. Recently, poly-l-lysine (PLL) has been reported as a common coating material for the improvement of cell adhesion. In this study, we hypothesized that PLL introduction in PLGA nanofiber would enhance the adhesion and proliferation of human adipose-derived stem cells (hASCs). To aim this, we fabricated PLGA/PLL nanofiber by electrospinning and evaluated cellular behavior of hASCs on it. PLGA/PLL nanofiber significantly promoted the attachment and proliferation of hASCs without cytotoxicity. Thus, PLGA/PLL nanofiber would be a useful scaffold to enhance adhesion and proliferation of stem cells and presumably to regulate differentiation of them in tissue engineering field.

Keywords

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