Environmental Analysis Health and Toxicology

The Korean Society of Environmental Health and Toxicology (환경독성보건학회)
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Exposure and Toxicity Assessment of Ultrafine Particles from Nearby Traffic in Urban Air in Seoul, Korea

  • Yang, Ji-Yeon (Institute for Environmental Research, Yonsei University College of Medicine) ;
  • Kim, Jin-Yong (Institute for Environmental Research, Yonsei University College of Medicine) ;
  • Jang, Ji-Young (Institute for Environmental Research, Yonsei University College of Medicine) ;
  • Lee, Gun-Woo (Institute for Environmental Research, Yonsei University College of Medicine) ;
  • Kim, Soo-Hwan (Institute for Environmental Research, Yonsei University College of Medicine) ;
  • Shin, Dong-Chun (Institute for Environmental Research, Yonsei University College of Medicine) ;
  • Lim, Young-Wook (Institute for Environmental Research, Yonsei University College of Medicine)
  • Received : 2013.02.18
  • Accepted : 2013.04.26
  • Published : 2013.01.02
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Abstract

Objectives We investigated the particle mass size distribution and chemical properties of air pollution particulate matter (PM) in the urban area and its capacity to induce cytotoxicity in human bronchial epithelial (BEAS-2B) cells. Methods To characterize the mass size distributions and chemical concentrations associated with urban PM, PM samples were collected by a 10-stage Micro-Orifice Uniform Deposit Impactor close to nearby traffic in an urban area from December 2007 to December 2009. PM samples for in vitro cytotoxicity testing were collected by a mini-volume air sampler with $PM_{10}$ and $PM_{2.5}$ inlets. Results The PM size distributions were bi-modal, peaking at 0.18 to 0.32 and 1.8 to $3.2{\mu}m$. The mass concentrations of the metals in fine particles (0.1 to $1.8{\mu}m$) accounted for 45.6 to 80.4% of the mass concentrations of metals in $PM_{10}$. The mass proportions of fine particles of the pollutants related to traffic emission, lead (80.4%), cadmium (69.0%), and chromium (63.8%) were higher than those of other metals. Iron was the dominant transition metal in the particles, accounting for 64.3% of the $PM_{10}$ mass in all the samples. We observed PM concentration-dependent cytotoxic effects on BEAS-2B cells. Conclusions We found that exposure to $PM_{2.5}$ and $PM_{10}$ from a nearby traffic area induced significant increases in protein expression of inflammatory cytokines (IL-6 and IL-8). The cell death rate and release of cytokines in response to the $PM_{2.5}$ treatment were higher than those with $PM_{10}$. The combined results support the hypothesis that ultrafine particles from vehicular sources can induce inflammatory responses related to environmental respiratory injury.

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References

  1. Harrison RM, Jones M. The chemical composition of airborne particles in the UK atmosphere. Sci Total Environ 1995;168(3): 195-214. https://doi.org/10.1016/0048-9697(95)04536-A
  2. Schwartz J, Norris G, Larson T, Sheppard L, Claiborne C, Koenig J. Episodes of high coarse particle concentrations are not associated with increased mortality. Environ Health Perspect 1999;107(5): 339-342. https://doi.org/10.1289/ehp.99107339
  3. Wilson WE, Suh HH. Fine particles and coarse particles: concentration relationships relevant to epidemiologic studies. J Air Waste Manag Assoc 1997;47(12):1238-1249. https://doi.org/10.1080/10473289.1997.10464074
  4. Oberdorster G, Gelein RM, Ferin J, Weiss B. Association of particulate air pollution and acute mortality: involvement of ultrafine particles? Inhal Toxicol 1995;7(1):111-124. https://doi.org/10.3109/08958379509014275
  5. Seaton A, MacNee W, Donaldson K, Godden D. Particulate air pollution and acute health effects. Lancet 1995;345(8943):176-178 https://doi.org/10.1016/S0140-6736(95)90173-6
  6. Peters A, Wichmann HE, Tuch T, Heinrich J, Heyder J. Respiratory effects are associated with the number of ultrafine particles. Am J Respir Crit Care Med 1997;155(4):1376-1383. https://doi.org/10.1164/ajrccm.155.4.9105082
  7. Donaldson K, Stone V, MacNee W. The toxicology of ultrafine particles. In: Howard V, Maynard RL; Royal Microscopical Society (Great Britain). Particulate matter: properties and effects upon health. Oxford: BIOS Scientific; 1999, p. 115-127.
  8. Donaldson K, Stone V. Current hypotheses on the mechanisms of toxicity of ultrafine particles. Ann Ist Super Sanita 2003;39(3):405-410
  9. Li N, Sioutas C, Cho A, Schmitz D, Misra C, Sempf J, et al. Ultrafine particulate pollutants induce oxidative stress and mitochondrial damage. Environ Health Perspect 2003;111(4):455-460.
  10. Li N, Xia T, Nel AE. The role of oxidative stress in ambient particulate matter-induced lung diseases and its implications in the toxicity of engineered nanoparticles. Free Radic Biol Med 2008;44(9): 1689-1699. https://doi.org/10.1016/j.freeradbiomed.2008.01.028
  11. Kelly FJ. Oxidative stress: its role in air pollution and adverse health effects. Occup Environ Med 2003;60(8):612-616. https://doi.org/10.1136/oem.60.8.612
  12. Akhtar US, McWhinney RD, Rastogi N, Abbatt JP, Evans GJ, Scott JA. Cytotoxic and proinflammatory effects of ambient and sourcerelated particulate matter (PM) in relation to the production of reactive oxygen species (ROS) and cytokine adsorption by particles. Inhal Toxicol 2010;22 Suppl 2:37-47. https://doi.org/10.3109/08958378.2010.518377
  13. Dreher KL, Jaskot RH, Lehmann JR, Richards JH, McGee JK, Ghio AJ, et al. Soluble transition metals mediate residual oil fly ash induced acute lung injury. J Toxicol Environ Health 1997;50(3): 285-305. https://doi.org/10.1080/009841097160492
  14. Saldiva PH, Clarke RW, Coull BA, Stearns RC, Lawrence J, Murthy GG, et al. Lung inflammation induced by concentrated ambient air particles is related to particle composition. Am J Respir Crit Care Med 2002;165(12):1610-1617. https://doi.org/10.1164/rccm.2106102
  15. Mar TF, Norris GA, Koenig JQ, Larson TV. Associations between air pollution and mortality in Phoenix, 1995-1997. Environ Health Perspect 2000;108(4):347-353. https://doi.org/10.1289/ehp.00108347
  16. Dominici F, McDermott A, Daniels M, Zeger SL, Samet JM. Revised analyses of the National Morbidity, Mortality, and Air Pollution Study: mortality among residents of 90 cities. J Toxicol Environ Health A 2005;68(13-14):1071-1092. https://doi.org/10.1080/15287390590935932
  17. Rosas Perez I, Serrano J, Alfaro-Moreno E, Baumgardner D, Garcia- Cuellar C, Martin Del Campo JM, et al. Relations between PM10 composition and cell toxicity: a multivariate and graphical approach. Chemosphere 2007;67(6):1218-1228. https://doi.org/10.1016/j.chemosphere.2006.10.078
  18. Winberry WT, Murphy NT, Riggin RM. Methods for determination of indoor air pollutants: EPA methods. Park Ridge, NJ: Noyes Data Corp.; 1993, p. 543-632.
  19. Zou C, Shen Z. An optimized in vitro assay for screening compounds that stimulate liver cell glucose utilization with low cytotoxicity. J Pharmacol Toxicol Methods 2007;56(1):58-62. https://doi.org/10.1016/j.vascn.2006.12.005
  20. Schnelle-Kreis J, Gebefugi I, Welzl G, Jaensch T, Kettrup A. Occurrence of particle-associated polycyclic aromatic compounds in ambient air of the city of Munich. Atmos Environ 2001;35(1):71-81.
  21. Kawanaka Y, Matsumoto E, Sakamoto K, Wang N, Yun SJ. Size distributions of mutagenic compounds and mutagenicity in atmospheric particulate matter collected with a low-pressure cascade impactor. Atmos Environ 2004;38(14):2125-2132. https://doi.org/10.1016/j.atmosenv.2004.01.021
  22. Fang GC, Wu YS, Wen CC, Lin CK, Huang SH, Rau JY, et al. Concentrations of nano and related ambient air pollutants at a traffic sampling site. Toxicol Ind Health 2005;21(10):259-271. https://doi.org/10.1191/0748233705th234oa
  23. Lough GC, Schauer JJ, Park JS, Shafer MM, Deminter JT, Weinstein JP. Emissions of metals associated with motor vehicle roadways. Environ Sci Technol 2005;39(3):826-836. https://doi.org/10.1021/es048715f
  24. Sørensen M, Schins RP, Hertel O, Loft S. Transition metals in personal samples of PM2.5 and oxidative stress in human volunteers. Cancer Epidemiol Biomarkers Prev 2005;14(5):1340-1343. https://doi.org/10.1158/1055-9965.EPI-04-0899
  25. Chen LC, Lippmann M. Effects of metals within ambient air particulate matter (PM) on human health. Inhal Toxicol 2009;21(1): 1-31.
  26. Rogge WF, Hildemann LM, Mazurek MA, Cass GR, Simonet BR. Sources of fine organic aerosol. 3. Road dust, tire debris, and organometallic brake lining dust: roads as sources and sinks. Environ Sci Technol 1993;27(9):1892-1904. https://doi.org/10.1021/es00046a019
  27. Yang HH, Chiang CF, Lee WJ, Hwang KP, Wu EM. Size distribution and dry deposition of road dust PAHs. Environ Int 1999; 25(5):585-597. https://doi.org/10.1016/S0160-4120(99)00036-7
  28. Goldberg MS, Burnett RT, Valois MF, Flegel K, Bailar JC 3rd, Brook J, et al. Associations between ambient air pollution and daily mortality among persons with congestive heart failure. Environ Res 2003;91(1):8-20. https://doi.org/10.1016/S0013-9351(02)00022-1
  29. Moolgavkar SH. Air pollution and daily mortality in two U.S. counties: season-specific analyses and exposure-response relationships. Inhal Toxicol 2003;15(9):877-907. https://doi.org/10.1080/08958370390215767
  30. Becker S, Dailey LA, Soukup JM, Grambow SC, Devlin RB, Huang YC. Seasonal variations in air pollution particle-induced inflammatory mediator release and oxidative stress. Environ Health Perspect 2005;113(8):1032-1038. https://doi.org/10.1289/ehp.7996
  31. Keatings VM, Collins PD, Scott DM, Barnes PJ. Differences in interleukin- 8 and tumor necrosis factor-alpha in induced sputum from patients with chronic obstructive pulmonary disease or asthma. Am J Respir Crit Care Med 1996;153(2):530-534. https://doi.org/10.1164/ajrccm.153.2.8564092
  32. Erger RA, Casale TB. Interleukin-8 is a potent mediator of eosinophil chemotaxis through endothelium and epithelium. Am J Physiol 1995;268(1 Pt 1):L117-L122.
  33. Fujii T, Hayashi S, Hogg JC, Vincent R, Van Eeden SF. Particulate matter induces cytokine expression in human bronchial epithelial cells. Am J Respir Cell Mol Biol 2001;25(3):265-271. https://doi.org/10.1165/ajrcmb.25.3.4445
  34. Li XY, Gilmour PS, Donaldson K, MacNee W. Free radical activity and pro-inflammatory effects of particulate air pollution (PM10) in vivo and in vitro. Thorax 1996;51(12):1216-1222. https://doi.org/10.1136/thx.51.12.1216
  35. Hoidal JR. Reactive oxygen species and cell signaling. Am J Respir Cell Mol Biol 2001;25(6):661-663. https://doi.org/10.1165/ajrcmb.25.6.f213
  36. Hetland RB, Cassee FR, Refsnes M, Schwarze PE, Lag M, Boere AJ, et al. Release of inflammatory cytokines, cell toxicity and apoptosis in epithelial lung cells after exposure to ambient air particles of different size fractions. Toxicol In Vitro 2004;18(2):203-212. https://doi.org/10.1016/S0887-2333(03)00142-5
  37. Seagrave J, Knall C, McDonald JD, Mauderly JL. Diesel particulate material binds and concentrates a proinflammatory cytokine that causes neutrophil migration. Inhal Toxicol 2004;16 Suppl 1:93-98.

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