생명과학회지 (Journal of Life Science)

  • 제23권1호
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  • Pages.95-101
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  • 2013
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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해안 생태계의 복원을 위하여 독도에 자생하는 식물로부터 분리된 내생진균류의 식물생장촉진활성과 유전학적 다양성

Plant Growth-Promoting Activity and Genetic Diversity of Endophytic Fungi Isolated from Native Plants in Dokdo Islands for Restoration of a Coastal Ecosystem

  • You, Young-Hyun (School of Life Science, Kyungpook National University) ;
  • Yoon, Hyeokjun (School of Life Science, Kyungpook National University) ;
  • Kim, Hyun (School of Life Science, Kyungpook National University) ;
  • Lim, Sung Hwan (Department of Biology, College of National Sciences, Kyungpook National University) ;
  • Shin, Jae-Ho (School of Applied Biosciences, Kyungpook National University) ;
  • Lee, In-Jung (School of Applied Biosciences, Kyungpook National University) ;
  • Choo, Yeon-Sik (Department of Biology, College of National Sciences, Kyungpook National University) ;
  • Kim, Jong-Guk (School of Life Science, Kyungpook National University)
  • 투고 : 2012.10.22
  • 심사 : 2013.01.03
  • 발행 : 2013.01.30
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초록

본 연구에서는 독도에 자생하는 5종의 식물, 비짜루, 갯괴불주머니, 왕김의 털, 땅채송화, 갯강아지풀을 채집하였다. 독도의 자생식물 뿌리로부터 33 주의 내생진균을 분리하였다. 분리된 모든 내생진균류들은 ITS1, 5.8s와 ITS2를 포함하는 ITS영역의 서열에 의해 분석되었다. 그리고 내생진균류의 배양여과액을 이용하여 난장이벼 유묘에 처리하여 식물생장촉진활성을 확인하였다. 그 결과, 땅채송화로부터 분리된 D-So-1-1 내생진균이 가장 우수한 식물생장촉진활성을 나타내었다. 그리고 분리된 모든 내생진균는 자낭균문의 Eurotiales (86%), Capnodiales (3%), Hypocreales (4%), 및 Incertae sedis (7%)에 속하는 것을 확인하였다. 그리고 내생진균류를 속으로 분류하였을 때, Aspergillus (12%), Cladosporium (3%), Eurotium (3%), Fusarium (18%), Microsphaeropsis (6%), 및 Penicillium (58%)인 것으로 나타났다.

In this study, plant samples of five species were collected from the Dokdo islands in South Korea. Plant samples such as Asparagus schoberioides, Corydalis platycarpa, Festuca rubra, Sedum oryzifolium, and Setaria viridis were collected from the Dongdo and Seodo. Endophytic fungal strains were isolated from the roots of five plants from the Dokdo islands. Thirty-three fungal strains were isolated from these native plants. All the endophytic fungi were analyzed by internal transcribed spacer (ITS) sequencing (ITS containing ITS1, 5.8s, and the ITS2 region). Waito-c rice seedlings were treated with fungal culture filtrates to test their plant growth-promoting activity. A bioassay of the D-So-1-1 fungal strain isolated from S. oryzifolium confirmed that it has the highest plant growth-promoting activity. All the endophytic fungi belong to four orders: Eurotiales (86%), Capnodiales (3%), Hypocreales (4%), and Incertae sedis (7%). The endophytic fungi were classified as Ascomycota, which contained Aspergillus (12%), Cladosporium (3%), Eurotium (3%), Fusarium (18%), Microsphaeropsis (6%), and Penicillium (58%) at the genus level.

키워드

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  2. Confirmation of Two Undescribed Fungal Species from Dokdo of Korea Based on Current Classification System Using Multi Loci vol.43, pp.4, 2015, https://doi.org/10.5941/MYCO.2015.43.4.392
  3. Metagenomic analysis of soil fungal communities on Ulleungdo and Dokdo Islands vol.61, pp.3, 2015, https://doi.org/10.2323/jgam.61.67
  4. Gibberellin A7 production by Aspergillus tubingensis YH103 and cultural characteristics of endophytic fungi isolated from Tetragonia tetragonoides in Dokdo islands vol.52, pp.1, 2016, https://doi.org/10.7845/kjm.2016.5071
  5. A strategy for securing unique microbial resources – focusing on Dokdo islands-derived microbial resources pp.2224-4662, 2018, https://doi.org/10.1163/22244662-20181024
  6. Four New Records of Ascomycete Species from Korea vol.46, pp.4, 2018, https://doi.org/10.1080/12298093.2018.1550169