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Coffee-like green tea의 인체암세포 및 정상세포에 대한 독성

Effect of Coffee-like Green Tea Preparation on Cytotoxicity of Human Cancer and Normal Cells

  • 문연규 (경상대학교 대학원 응용생명과학부) ;
  • 권애경 (경상대학교 대학원 응용생명과학부) ;
  • 김종철 ((재)하동녹차연구소) ;
  • 박한민 (경상대학교 환경재료과학과) ;
  • 조용운 (경남과학기술대학 제약공학과) ;
  • 정관주 ((재)바이오21센터) ;
  • 하영래 (경상대학교 대학원 응용생명과학부)
  • Moon, Yean Guy (Division of Applied Life Science (BK21 Program), Graduate School, and Institute of Agriculture & Life Science, Gyeongsang National University) ;
  • Kwon, Jung Min (Division of Applied Life Science (BK21 Program), Graduate School, and Institute of Agriculture & Life Science, Gyeongsang National University) ;
  • Kim, Jong Cheol (Institute of Hadong Green Tea) ;
  • Park, Han Min (Department of Environmental Materials Science, Gyeongsang National University) ;
  • Cho, Yong Un (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology) ;
  • Jung, Kwan Ju (Bio21 center) ;
  • Ha, Yeong Lea (Division of Applied Life Science (BK21 Program), Graduate School, and Institute of Agriculture & Life Science, Gyeongsang National University)
  • 투고 : 2012.10.07
  • 심사 : 2013.01.10
  • 발행 : 2013.01.30

초록

커피와 유사한 향미기를 갖는 녹차(Coffee-like green tea: CLGT)를 제조하고 이의 인체 유방암세포 주 MCF-7, 인체 전립선암세포 주 PC-3, 인체 신경모세포 주 SK-N-SH 및 쥐 심근세포 주 H9c2에 대한 세포독성을 연구하였다. 녹차엽(GTL)을 $240^{\circ}C$에서 1시간 roasting한 녹차가 커피와 가장 유사한 향미를 나타내었다. CLGT는 GTL과 비교하여 인체 암세포 및 정상세포와 쥐 심근세포에 대한 세포독성 차이가 없었다. GTL의 roasting은 ECG 등의 catechin 성분과 total protein은 유의성 있게 감소시켰지만, total phenol 및 total sugar는 유의성 있는 감소시키지 않아, 이와 같은 화합물 조성의 변화가 GTL에 커피유사 향미를 제공하였을 것이다. 따라서 이들 결과는 CLGT이 인체에 안전하여 커피 대용품으로 사용할 수 있음을 의미한다.

The cytotoxicity of coffee-like green tea (CLGT) was determined in a human breast cancer cell line, MCF-7; a human prostate cancer cell clone, PC-3; a human neuroblastoma cell line, SK-N-SH; and a rat cardiomyoblast cell line, H9c2, with reference to green tea leaves (GTL). The CLGT was prepared by roasting the GTL for 60 min at $240^{\circ}C$ in a temperature-controlled frying pan. The CLGT preparation imitated the flavor and taste characteristics of coffee fairly well according to sensory analysis. The CLGT preparation had no adverse cytotoxic effects on the cancer cells or the normal cells compared to GTL. No significant change in the antioxidant activity was seen in the CLGT preparation compared to that of GTL. The amount of total protein, sugar, and phenolic compounds was reduced in the preparation relative to those in GTL, a fact that might explain the coffee-like flavor and/or taste characteristics of the CLGT preparation. These results suggest that CLGT prepared by roasting GTL for 60 min at $240^{\circ}C$ does not show any adverse effects on cancer cells and normal cells compared to GTL. They imply that CLGT could be safe for human consumption.

키워드

참고문헌

  1. Agarwal, R., Katiyar, S. K., Zaidi, S. I. A. and Mukhtar, H. 1992. Inhibition of skin tumor promoter-caused induction of epidermal ornithine decarboxylase in SENCAR mice by polyphenolic fraction isolated from green tea and its individual epicatechin derivatives. Cancer Res 52, 3582-3588.
  2. Aronson, D. 2004. Pharmacological prevention of cardiovascular aging-targeting the Maillard reaction. Br J Pharmacol 142, 1055-1058. https://doi.org/10.1038/sj.bjp.0705832
  3. Babich, H., Krupka, M. E., Nissim, H. A. and Zuckerbraun, H. L. 2005. Differential in vitro cytotoxicity of (-)-epicatechin gallate (ECG) to cancer and normal cells from the human oral cavity. Toxicology in Vitro 19, 231-242. https://doi.org/10.1016/j.tiv.2004.09.001
  4. Brand-Williams, W., Cuvelier, M. E. and Berset, C. 1995. Use of a free radical method to evaluate antioxidant activity. Technology 28, 25-30.
  5. Choi, S. H. 2010. Effects of pyrazines, reaction products of Maillard reaction on flavor of green tea and substitution tea. J Korean Tea Soc. 16, 1-7.
  6. Conney, A. H., Wang, Z. Y., Huang, M. T., Ho, C. T. and Yang, C. S. 1991. Inhibitory effect of green tea on tumorigenesis by chemicals and ultraviolet light. Prev Med 21, 361-369.
  7. Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A. and Smith, F. 1956, Calorimetric method for determination of sugars and related substances. Analyt Chim 28, 350-356. https://doi.org/10.1021/ac60111a017
  8. El-Shahawi, M. S., Hamza, A., Bahaffi, S. O., Al-Sibaai, A. A. and Abduljabbar, T. N. 2012. Analysis of some selected catechins and caffeine in green tea by high performance liquid chromatography. Food Chem 134, 2268-2275. https://doi.org/10.1016/j.foodchem.2012.03.039
  9. Houessou, J. K., Maloug, S., Leveque, A. S., Delteil, C., Heyd, B. and Camel, V. 2007. Effect of roasting conditions on the polycyclic aromatic hydrocarbon content in ground arabica coffee and coffee brew. J Agric Food Chem 55, 9719-9726. https://doi.org/10.1021/jf071745s
  10. Kaur, S., Greaves, P., Cooke, D. N., Edwards, R., Steward, W. P., Gescher, A. J. and Marczylo, T. H. 2007. Breast cancer prevention by green tea catechins and black tea theaflavins in the C3(1) SV40. t,t antigen transgenic mouse model is accompanied by increased apoptosis and a decrease in oxidative DNA adducts. J Agric Food Chem 55, 3378-3385. https://doi.org/10.1021/jf0633342
  11. Kenji, K. and Hideki, M. 2002. Identification of potent odorants in different green tea varieties using flavor dilution technique. J Agric Food Chem 50, 5660-5663. https://doi.org/10.1021/jf020498j
  12. Kikugawa, K., Kato, T. and Takahashi, S. 1989. Possible presence of 2-amino-3,4-dimethyl imidazo[4,5-f]quinoline and other heterocyclic amine-like mutagens in roasted coffee beans. J Agric Food Chem 37, 881-886. https://doi.org/10.1021/jf00088a011
  13. Kim, E. S., Liang, Y. R., Jin, J., Sun, Q. F., Lu, J. L., Du, Y. Y. and Lin, C. 2006. Impact of heating on chemical compositions of green tea liquor. Food Chem 103, 1263-1267.
  14. Kim, Y. K., Kim, H. N., Choi, J. Y. and Baig, J. H. 2007. Coffee-flavored mixed tea with excellent taste and preparing method thereof. Patent pending, 10-2007-0102701, Korea.
  15. Kumazawa, K. and Masuda, H. 2003. Investigation of the change in the flavor of a coffee drink during heat processing. J Agric Food Chem 51, 3274-3278.
  16. Lee, G. D., Jeong, Y. J., Park, N. Y. and Kwon, J. H. 1999. Monitoring for the color formation of a Doraji tea by soaking of threonine and sucrose solution and roasting. Korean J Food Sci Technol 31, 938-944.
  17. Lee, S. I., Jeon, D. B., Kim, J. S., Jung, I. S., Hong, Y. S., Seo, H. Y., Park, K. H. and Kim, K. S. 2011. Analysis of volatile flavor components in fresh leaves and roasted green tea. J Korean Tea Soc 17, 46-53.
  18. Lyman, D. J., Benck, R., Dell, S., Merle, S. and Jacqueline, M. W. 2003. FTIR-ATR analysis of brewed coffee: effect of roasting conditions. J Agric Food Chem 51, 3268-3272. https://doi.org/10.1021/jf0209793
  19. Miller, A. G., Meade, S. J. and Gerrard, J. A. 2003. New insights into protein cross linking via the Maillard reaction: structural requirement, the effect on enzyme function, and predicted efficacy of cross linking inhibitors as anti-aging therapeutics. Bioorg Med Chem 11, 843-852. https://doi.org/10.1016/S0968-0896(02)00565-5
  20. Nishigaki, R., Watanabe, T., Kajimoto, T., Tada, A., Takamura-Enya, T., Enomoto, S., Nukaya, H., Terao, Y., Muroyama, A., Ozeki, M., Node, M., Hasei, T., Totsuka, Y. and Wakabayashi, K. 2009. Isolation and identification of a novel aromatic amine mutagen produced by the Maillard reaction. Chem Res Toxicol 22, 1588-1593. https://doi.org/10.1021/tx900119j
  21. Pan, M. H., Kang, H. J., Chen, W. J., Lo, C. Y., Li, S., Sang, S. and Ho, C. T. 2008. Induction of apoptosis by acetylated black tea polyphenol through reactive oxygen species production, cytochrome c release, and caspases activation in human leukemia HL-60 cells, pp. 345-361, In Shibamoto, T., Kanazawa, K., Shahidi, F. and Ho, C. T. (eds.), Functional Food and Health, Vol. 993, American Chemistry Society.
  22. Rakib, M. A., Kim, Y. S., Jang, W. J., Jang, J. S., Kang, S. J. and Ha, Y. L. 2011. Preventive effect of t,t-conjugated linoleic acid on 12-O-tetradecanoyl- phorbol-13-acetate-induced inhibition of gap junctional intercellular communication in human mammary epithelial MCF-10A Cells. J Agric Food Chem 59, 4164-4170. https://doi.org/10.1021/jf1046909
  23. Park, J. H., Nam, S. H., Choi, J. J. and Hwang, H. S. 2010. Comparison of component content between envirnomental- frendly and conventional green tea. J Korean Tea Soc 16, 106-110.
  24. Park, N. Y., Jeong Y. J. and Kwon, J. H. 2007. Changes in flavor compounds of Polygonatum odoratum root during roasting. Korean J Food Sci Technol 39, 99-103.
  25. Sakagami, H., Asano, K., Hara, Y. and Shimamura, T. 1992. Stimulation of human monocyte and polymorphonuclear cell iodination and interleukin-1 production by epigallocatechin gallate. J Leukoc Biol 51, 478-83.
  26. Singleton, V. L. and Rossi, J. A, Jr. 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Viticult 16, 144-158.
  27. Summa, C., McCourt, J., Cammerer, B, Fiala, A., Probst, M., Kun, S., Anklam, E. and Wagner, K. H. 2008. Radical scavenging activity, anti-bacterial and mutagenic effects of cocoa bean Maillard reaction products with degree of roasting. Mol Nutr Food Res 52, 342-351. https://doi.org/10.1002/mnfr.200700403
  28. Wei, F., Furihata, K., Koda, M., Hu, F., Miyakawa, T. and Tanokura, M. 2012. Roasting process of coffee beans as studied by nuclear magnetic resonance: time course of changes in composition. J Agric Food Chem 60, 1005-1012. https://doi.org/10.1021/jf205315r
  29. You, J. O. 2010. A study on usage pattern of green tea and analysis for its expansion factor. Korean J Food Sci Technol 44, 931-934.