생명과학회지 (Journal of Life Science)

  • 제23권1호
  • /
  • Pages.38-43
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  • 2013
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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애기뿔소똥구리 유래 CopA3합성 펩타이드의 항염증 효능에 관한 연구

Study of Anti-inflammatory Effect of CopA3 Peptide Derived from Copris tripartitus

  • 김현정 (대구한의대학교 화장품약리학과) ;
  • 김동희 (한국한방산업진흥원) ;
  • 이진영 (호서대학교 한방화장품과 학과) ;
  • 황재삼 (국립농업과학원 농업생물부) ;
  • 이준하 (국립농업과학원 농업생물부) ;
  • 이슬기 (대구한의대학교 화장품약리학과) ;
  • 정현국 (대구한의대학교 화장품약리학과) ;
  • 안봉전 (대구한의대학교 화장품약리학과)
  • Kim, Hyeon-Jeong (Department of Cosmeceutical Science, Daegu Haany University) ;
  • Kim, Dong-Hee (Korea Promotion Institute for Traditional Medicine Industry) ;
  • Lee, Jin-Young (Department of Herbal Cosmetic Science, Hoseo University) ;
  • Hwang, Jae-Sam (Department of Agricultural Biology, National Academy of Agricultural Science) ;
  • Lee, Joon-Ha (Department of Agricultural Biology, National Academy of Agricultural Science) ;
  • Lee, Seul-Gi (Department of Cosmeceutical Science, Daegu Haany University) ;
  • Jeong, Hyeon-Guk (Department of Cosmeceutical Science, Daegu Haany University) ;
  • An, Bong-Jeun (Department of Cosmeceutical Science, Daegu Haany University)
  • 투고 : 2012.10.31
  • 심사 : 2013.01.03
  • 발행 : 2013.01.30
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초록

CopA3를 이용하여 피부 염증에 대하여 연구를 하였다. 산화질소와 cytokine의 생산은 면역세포의 대표적인 염증인자이다. 세포는 LPS 처리 후 한 시간 뒤에 CopA3를 처리하였다. 세포 독성이 나타나지 않는 농도인 5, 25, 50, 100 ${\mu}g/ml$를 사용하였다. CopA3는 NO, TNF-${\alpha}$, IL-$1{\beta}$, IL-6, iNOS, COX-2의 생성을 저해 시켰다. iNOS와 COX-2 역시 100 ${\mu}g/ml$의 농도에서 각각 54%, 65%가 저해가 되었다. 게다가 CopA3는 염증성 사이토 카인인 TNF-${\alpha}$, IL-$1{\beta}$, IL-6의 생성을 감소 시켰다. 이러한 결과로 CopA3는 염증 예방과 치료에 효과적임을 확인 할 수 있었다.

The objective of this study was to evaluate the effect of the synthetic CopA3 peptide of Copris tripartitus on skin inflammation. Regulatory mechanisms of cytokines and nitric oxide (NO) are involved in the immunological activity of RAW 264.7 cells. Tested cells were treated with different concentrations of CopA3 and further cultured for an appropriate time after lipopolyssacharide (LPS) addition. During the entire experimental period, 5, 25, 50, and 100 ${\mu}g/ml$ of CopA3 had no cytotoxicity. At these concentrations, CopA3 inhibited tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), interleukin-$1{\beta}$ (IL-$1{\beta}$), and interleukin-6 (IL-6). CopA3 also inhibited the expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2). CopA3 inhibited the activity of iNOS and COX-2 by 41% and 59%, respectively, at 100 ${\mu}g/ml$. In addition, CopA3 reduced the release of inflammatory cytokines including TNF-${\alpha}$, IL-$1{\beta}$, and IL-6. These results suggest that CopA3 may have significant effects on inflammatory factors and that it may be a potential anti-inflammatory therapeutic agent.

키워드

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피인용 문헌

  1. Anti-Inflammatory Effect of Violapyrones B and C from a Marine-derivedStreptomycessp. vol.21, pp.4, 2015, https://doi.org/10.20307/nps.2015.21.4.251
  2. in RAW 264.7 cells vol.51, pp.4, 2018, https://doi.org/10.4163/jnh.2018.51.4.323