Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Phylogenetic Analysis of Korean Black Cattle Based on the Mitochondrial Cytochrome b Gene

mtDNA cytochrome b에 기초한 한국흑우의 계통유전학적 분석

  • 김재환 (농촌진흥청 국립축산과학원) ;
  • 변미정 (농촌진흥청 국립축산과학원) ;
  • 김명직 (농촌진흥청 국립축산과학원) ;
  • 서상원 (농촌진흥청 국립축산과학원) ;
  • 김영신 (농촌진흥청 국립축산과학원) ;
  • 고응규 (농촌진흥청 국립축산과학원) ;
  • 김성우 (농촌진흥청 국립축산과학원) ;
  • 정경섭 (충북축산위생연구소) ;
  • 김동훈 (농촌진흥청 국립축산과학원) ;
  • 최성복 (농촌진흥청 국립축산과학원)
  • Received : 2012.09.13
  • Accepted : 2013.01.03
  • Published : 2013.01.30
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Abstract

The purpose of this study was to identify genetic polymorphisms of the mitochondrial cytochrome b (mtDNA cyt b) gene in Korean black (KB) cattle breed and to analyze the genetic relationship between the KB and other breeds. We determined the complete sequence of the mtDNA cyt b gene in 38 KB cattle. We also analyzed their genetic diversity, and phylogenetic analysis was performed by comparison with Korean cattle (KC, called Hanwoo) and breeds from China and Japan. A nucleotide substitution was detected in the KB cattle, and two haplotypes were defined. In the neighbor-joining (NJ) tree, the haplotypes of KB were located in Bos taurus lineage with those of KC, Japanese black (JB), Yanbian and Zaosheng breeds. However, the haplotypes of Chinese breeds, excluding Yanbian and Zaosheng, were separated into B. taurus and B. indicus lineages. In the NJ tree of breeds based on Dxy genetic distances, Chinese breeds mixed with B. taurus and B. indicus lineages were located between B. indicus and B. taurus lineages. KB was contained within B. taurus lineage and was determined to be genetically more closely related to two Chinese (Yanbian and Zaosheng) breeds than to KC and JB. The haplotype distribution and the results of the phylogenetic analysis suggest that KB and KC have genetic differences in their mtDNA cyt b gene sequences.

본 연구는 mtDNA cytochrome b (Cyt b) 유전자 서열을 토대로 한국흑우의 유전적 다양성 및 계통유전학적 위치를 파악하기 위하여 실시하였다. 한국흑우 38두로부터 결정된 mtDNA Cyt b 유전자 전체서열 내에서 염기삽입 및 결실 없이 1개의 silent mutation이 동정되었다. 또한 2개의 haplotype으로 분류되었고, 염기변이율 및 haplotype 다양성지수에 기초한 한국흑우의 유전적 다양성은 기존에 보고된 중국 품종에 비해 낮게 나타났다. 한국, 일본, 중국에 분포하고 있는 12품종 101개 서열을 수집하여 한국흑우와의 유연관계를 확인하였다. 한국흑우에서 분류된 2개의 haplotype은 모두 B. taurus 계열에 포함되었으며, 한우, 일본흑우, Yanbian, Zaosheng 등 4개 품종과 하나의 그룹을 형성하였다. 또한 품종별 Dxy 유전거리 산출 결과, 한국흑우는 한우 및 일본흑우에 비해서 중국의 Yanbian, Zaosheng 품종과 더 가까운 유연관계를 보였다. 본 연구의 결과는 가축유전자원으로서 한국흑우의 보존 및 유전적 특성 구명을 위한 중요한 자료로 활용이 가능할 것으로 사료된다.

Keywords

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