Development and Reproduction (한국발생생물학회지:발생과생식)

The Korean Society of Developmental Biology (한국발생생물학회)
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Differential Growth of the Reproductive Organs during the Peripubertal Period in Male Rats

  • Han, Seung Hee (Department of Life Science, Sangmyung University) ;
  • Lee, Sung-Ho (Department of Life Science, Sangmyung University)
  • Received : 2013.11.27
  • Accepted : 2013.12.21
  • Published : 2013.12.31
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Abstract

In mammals, puberty is a process of acquiring reproductive competence, triggering by activation of hypothalamic kisspeptin (KiSS)-gonadotropin releasing hormone (GnRH) neuronal circuit. During peripubertal period, not only the external genitalia but the internal reproductive organs have to be matured in response to the hormonal signals from hypothalamic-pituitary-gonadal (H-P-G) axis. In the present study, we evaluated the maturation of male rat accessory sex organs during the peripubertal period using tissue weight measurement, histological analysis and RT-PCR assay. Male rats were sacrificed at 25, 30, 35, 40, 45, 50, and 70 postnatal days (PND). The rat accessory sex organs exhibited differential growth patterns compared to those of non-reproductive organs. The growth rate of the accessory sex organs were much higher than the those of non-reproductive organs. Also, the growth spurts occurred differentially even among the accessory sex organs; the order of prepubertal organ growth spurts is testis = epididymis > seminal vesicle = prostate. Histological study revealed that the presence of sperms in seminiferous tubules and epididymal ducts at day 50, indicating the puberty onset. The number of duct and the volume of duct in epididymis and prostate were inversely correlated during the experimental period. Our RT-PCR revealed that the levels of hypothalamic GnRH transcript were increased significantly on PND 40, suggesting the activation of hypothalamic GnRH pulse-generator before puberty onset. Studies on the peripubertal male accessory sex organs will provide useful references on the growth regulation mechanism which is differentially regulated during the period in androgen-sensitive organs. The detailed references will render easier development of endocrine disruption assay.

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References

  1. Blystone CR, Lambright CS, Furr J, Wilson VS, Gray LE Jr (2007) Iprodione delays male rat pubertal development, reduces serum testosterone levels, and decreases ex vivo testicular testosterone production. Toxicol Lett 174(1-3):74-81. https://doi.org/10.1016/j.toxlet.2007.08.010
  2. Dart DA, Waxman J, Aboagye EO, Bevan CL (2013) Visualising androgen receptor activity in male and female mice. PLoS One 8(8):e71694. doi: 10.1371/ journal.pone.0071694.
  3. Kim HS, Shin JH, Moon HJ, Kim TS, Kang IH, Seok JH, Kim IY, Park KL, Han SY (2002) Evaluation of the 20-day pubertal female assay in Sprague-Dawley rats treated with DES, tamoxifen, testosterone, and flutamide. Toxicol Sci 67(1):52-62. https://doi.org/10.1093/toxsci/67.1.52
  4. Macleod DJ, Sharpe RM, Welsh M, Fisken M, Scott HM, Hutchison GR, Drake AJ, van den Driesche S (2010) Androgen action in the masculinization programming window and development of male reproductive organs. Int J Androl 33(2):279-287. https://doi.org/10.1111/j.1365-2605.2009.01005.x
  5. Navarro VM, Castellano JM, Fernandez-Fernandez R, Barreiro ML, Roa J, Sanchez-Criado JE, Aguilar E, Dieguez C, Pinilla L, Tena-Sempere M (2004) Developmental and hormonally regulated messenger ribonucleic acid expression of KiSS-1 and its putative receptor, GPR54, in rat hypothalamus and potent luteinizing hormone-releasing activity of KiSS-1 peptide. Endocrinology 145(10):4565-4574. https://doi.org/10.1210/en.2004-0413
  6. Navarro VM, Castellano JM, Garcia-Galiano D, Tena-Sempere M (2007) Neuroendocrine factors in the initition of puberty: the emergent role of kisspeptin. Rev Endocr Metab Disord 8(1):11-20. https://doi.org/10.1007/s11154-007-9028-2
  7. Nishino T, Wedel T, Schmitt O, Buhlmeyer K, Schonfelder M, Hirtreiter C, Schulz T, Kühnel W, Michna H. (2004) Androgen-dependent morphology of prostates and seminal vesicles in the Hershberger assay: evaluation of immunohistochemical and morphometric parameters. Ann Anat 186(3):247-253. https://doi.org/10.1016/S0940-9602(04)80011-6
  8. Okahashi N, Sano M, Miyata K, Tamano S, Higuchi H, Kamita Y, Seki T (2005) Lack of evidence for endocrine disrupting effects in rats exposed to fenitrothion in utero and from weaning to maturation. Toxicology 206(1):17-31. https://doi.org/10.1016/j.tox.2004.04.020
  9. Rivest RW (1991) Sexual maturation in female rats: hereditary, developmental and environmental aspects. Experientia 47(10):1027-1038.
  10. Rossi R, Zatelli MC, Valentini A, Cavazzini P, Fallo F, del Senno L, degli Uberti EC (1998) Evidence for androgen receptor gene expression and growth inhibitory effect of dihydrotestosterone on human adrenocortical cells. J Endocrinol 159(3):373-380. https://doi.org/10.1677/joe.0.1590373
  11. Sakabe K, Kawashima I, Urano R, Seiki K, Itoh T (1994) Effects of sex steroids on the proliferation of thymic epithelial cells in a culture model: a role of protein kinase C. Immunol Cell Biol 72(3):193-199. https://doi.org/10.1038/icb.1994.29
  12. Stefani S, Aguiari GL, Bozza A, Maestri I, Magri E, Cavazzini P, Piva R, del Senno L (1994) Androgen responsiveness and androgen receptor gene expression in human kidney cells in continuous culture. Biochem Mol Biol Int 32(4):597-604.
  13. van Weissenbruch MM, Engelbregt MJ, Veening MA, Delemarre-van de Waal HA (2005) Fetal nutrition and timing of puberty. Endocr Dev 8:15-33. https://doi.org/10.1159/000084084
  14. Vomachka AJ, Greenwald GS (1979) The development of gonadotropin and steroid hormone patterns in male and female hamsters from birth to puberty. Endocrinology 105(4):960-966. https://doi.org/10.1210/endo-105-4-960
  15. Wheeler MD (1991) Physical changes of puberty. Endocrinol Metab Clin North Am 20(1):1-14.
  16. Yadav N, Heemers HV (2012) Androgen action in the prostate gland. Minerva Urol Nefrol 64(1):35-49.