한국발생생물학회지:발생과생식 (Development and Reproduction)

  • 제17권4호
  • /
  • Pages.409-420
  • /
  • 2013
  • /
  • 2465-9525(pISSN)
  • /
  • 2465-9541(eISSN)
한국발생생물학회 (The Korean Society of Developmental Biology)
권호 표지
DOI QR코드

DOI QR Code

A Role of Cell Adhesion Molecules and Gelatinases in Human Serum-Induced Aggregation of Human Eyelid-Derived Stem Cells In Vitro

  • Yang, Hyejin (Department of Biotechnology, Seoul Women's University) ;
  • Lim, Yoon Hwa (Department of Biotechnology, Seoul Women's University) ;
  • Yun, Sujin (Department of Biotechnology, Seoul Women's University) ;
  • Yoon, A Young (Department of Biotechnology, Seoul Women's University) ;
  • Kim, Haekwon (Department of Biotechnology, Seoul Women's University)
  • 투고 : 2013.11.26
  • 심사 : 2013.12.20
  • 발행 : 2013.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Human serum (HS) has been reported to induce aggregation of human eyelid adipose-derived stem cells (HEACs) during high-density culture in vitro. The present study focused on the role of cell adhesion molecules and gelatinases during HS-induced aggregation of HEACs. HS-induced aggregation occurred between 9-15 days of culture. Cells aggregated by HS medium (HS-agg) showed stronger expression of ${\alpha}2$, ${\alpha}2B$, ${\alpha}X$, and CEACAM1 genes compared to non-aggregated cells in HS medium (HS-ex) or in control FBS-cultured cells. HS-agg were distinctly labeled with antibodies against ${\alpha}2$, ${\alpha}2B$, and ${\alpha}X$ proteins. Western blot results demonstrated that the two integrin proteins were greatly expressed in HS-agg compared to HS-ex and control FBS-cultured cells. Treatment of HEACs with anti-integrin ${\alpha}2$ antibody during culture in HS medium delayed aggregation formation. HS-agg exhibited strong expression of MMP1 and MMP9 compared to HS-ex or FBS-cultured cells. Conditioned media from HS-culture showed remarkable increase of MMP9 gelatinolytic activity in comparison to those from FBS-culture. However, there was no change of TIMP mRNA expression in relation to the HS-induced aggregation. Based on these results, it is suggested that integrin ${\alpha}2$, ${\alpha}2B$, and ${\alpha}X$, and MMP9 might play an important role in the HS-induced aggregation of HEACs.

키워드

참고문헌

  1. Bianco P, Robey PG, Simmons PJ (2008) Mesenchymal stem cells: revisiting history, concepts, and assays. Cell Stem Cell 2:313-319. https://doi.org/10.1016/j.stem.2008.03.002
  2. Bieback K, Ha VA, Hecker A, Grassl M, Kinzebach S, Solz H, Sticht C, Klüter H, Bugert P (2010) Altered gene expression in human adipose stem cells cultured with fetal bovine serum compared to human supplements. Tissue Eng Part A 16:3467-3484. https://doi.org/10.1089/ten.tea.2009.0727
  3. Bieback K, Hecker A, Kocaomer A, Lannert H, Schallmoser K, Strunk D, Klüter H (2009) Human alternatives to fetal bovine serum for the expansion of mesenchymal stromal cells from bone marrow. Stem Cells 27:2331- 2341. https://doi.org/10.1002/stem.139
  4. Bieback K, Hecker A, Schlechter T, Hofmann I, Brousos N, Redmer T, Besser D, Kluter H, Muller AM, Becker M (2012) Replicative aging and differentiation potential of human adipose tissue-derived mesenchymal stromal cells expanded in pooled human or fetal bovine serum. Cytotherapy 14:570-583. https://doi.org/10.3109/14653249.2011.652809
  5. Brooke G, Tong H, Levesque JP, Atkinson K (2008) Molecular trafficking mechanisms of multipotent mesenchymal stem cells derived from human bone marrow and placenta. Stem Cells Dev 17:929-940. https://doi.org/10.1089/scd.2007.0156
  6. Carrion B, Kong YP, Kaigler D, Putnam AJ (2013) Bone marrow-derived mesenchymal stem cells enhance angiogenesis via their ${\alpha}6{\beta}1$ integrin receptor. Exp Cell Res 319:2964-2476. https://doi.org/10.1016/j.yexcr.2013.09.007
  7. Cheng NC, Chen SY, Li JR, Young TH (2013) Short-term spheroid formation enhances the regenerative capacity of adipose-derived stem cells by promoting stemness, angiogenesis, and chemotaxis. Stem Cells Transl Med 2:584-594. https://doi.org/10.5966/sctm.2013-0007
  8. De Becker A, Van Hummelen P, Bakkus M, Vande Broek I, De Wever J, De Waele M, Van Riet I (2007) Migration of culture-expanded human mesenchymal stem cells through bone marrow endothelium is regulated by matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-3. Haematologica 92:440-449. https://doi.org/10.3324/haematol.10475
  9. Dumin JA, Dickeson SK, Stricker TP, Bhattacharyya- Pakrasi M, Roby JD, Santoro SA, Parks WC (2001) Pro-collagenase-1 (matrix metalloproteinase-1) binds the alpha(2)beta(1) integrin upon release from keratinocytes migrating on type I collagen. J Biol Chem 276:29368-29374. https://doi.org/10.1074/jbc.M104179200
  10. Frith JE, Mills RJ, Hudson JE, Cooper-White JJ (2012) Tailored integrin-extracellular matrix interactions to direct human mesenchymal stem cell differentiation. Stem Cells Dev 21:2442-2456. https://doi.org/10.1089/scd.2011.0615
  11. Gellersen B, Wolf A, Kruse M, Schwenke M, Bamberger AM (2013) Human endometrial stromal cell-trophoblast interactions: mutual stimulation of chemotactic migration and promigratory roles of cell surface molecules CD82 and CEACAM1. Biol Reprod 88:80. https://doi.org/10.1095/biolreprod.112.106724
  12. Granero-Molto F, Weis JA, Miga MI, Landis B, Myers TJ, O'Rear L, Longobardi L, Jansen ED, Mortlock DP, Spagnoli A (2009) Regenerative effects of transplanted mesenchymal stem cells in fracture healing. Stem Cells 27:1887-1898. https://doi.org/10.1002/stem.103
  13. Jarvis GE, Bihan D, Hamaia S, Pugh N, Ghevaert CJ, Pearce AC, Hughes CE, Watson SP, Ware J, Rudd CE, Farndale RW (2012) A role for adhesion and degranulationpromoting adapter protein in collagen-induced platelet activation mediated via integrin $\alpha$(2) $\beta$(1). J Thromb Haemost 10:268-277. https://doi.org/10.1111/j.1538-7836.2011.04567.x
  14. Kaneider NC, Mosheimer B, Gunther A, Feistritzer C, Wiedermann CJ (2010) Enhancement of fibrinogentriggered pro-coagulant activation of monocytes in vitro by matrix metalloproteinase-9. Thromb J 8:1-7.
  15. Kang HM, Kim J, Park S, Kim J, Kim H, Kin KS, Kim EJ, Seo SI, Kang SG, Lee JE, Lim H (2009) Insulinsecreting cells from human eyelid-derived stem cells alleviate type I diabetes in immunocompetent mice. Stem Cells Dev 27:1999-2008. https://doi.org/10.1002/stem.127
  16. Kawai K, Xue F, Takahara T, Kudo H, Yata Y, Zhang W, Sugiyama T (2012) Matrix metalloproteinase-9 contributes to the mobilization of bone marrow cells in the injured liver. Cell Transplant 21:453-464. https://doi.org/10.3727/096368911X605367
  17. Kocaoemer A, Kern S, Kluter H, Bieback K (2007) Human AB serum and thrombin-activated platelet-rich plasma are suitable alternatives to fetal calf serum for the expansion of mesenchymal stem cells from adipose tissue. Stem Cells 25:1270-1278. https://doi.org/10.1634/stemcells.2006-0627
  18. McBane RD 2nd, Karnicki K, Wysokinski WE (2013) Platelet recruitment to venous stent thrombi. J Thromb Thrombolysis 36:442-427. https://doi.org/10.1007/s11239-013-0924-x
  19. Mizuno H, Tobita M, Uysal AC (2012) Concise review: Adipose-derived stem cells as a novel tool for future regenerative medicine. Stem Cells 30:804-810. https://doi.org/10.1002/stem.1076
  20. Neumann FJ, Blasini R, Schmitt C, Alt E, Dirschinger J, Gawaz M, Kastrati A, Schomig A (1998) Effect of glycoprotein IIb/IIIa receptor blockade on recovery of coronary flow and left ventricular function after the placement of coronary-artery stents in acute myocardial infarction. Circulation 98:2695-2701. https://doi.org/10.1161/01.CIR.98.24.2695
  21. Prowse AB, Chong F, Gray PP, Munro TP (2011) Stem cell integrins: implications for ex-vivo culture and cellular therapies. Stem Cell Res 6:1-12. https://doi.org/10.1016/j.scr.2010.09.005
  22. Ries C, Egea V, Karow M, Kolb H, Jochum M, Neth P. (2007) MMP-2, MT1-MMP, and TIMP-2 are essential for the invasive capacity of human mesenchymal stem cells: differential regulation by inflammatory cytokines. Blood 109:4055-4063. https://doi.org/10.1182/blood-2006-10-051060
  23. Sarkar D, Spencer JA, Phillips JA, Zhao W, Schafer S, Spelke DP, Mortensen LJ, Ruiz JP, Vemula PK, Sridharan R, Kumar S, Karnik R, Lin CP, Karp JM (2011) Engineered cell homing. Blood 118:184-191. https://doi.org/10.1182/blood-2010-10-311464
  24. Schnickmann S, Camacho-Trullio D, Bissinger M, Eils R, Angel P, Schirmacher P, Szabowski A, Breuhahn K (2009) AP-1-controlled hepatocyte growth factor activation promotes keratinocyte migration via CEACAM1 and urokinase plasminogen activator/urokinase plasminogen receptor. J Invest Dermatol 129:1140-1148. https://doi.org/10.1038/jid.2008.350
  25. Song Y, Yun S, Yang H, Yoon A, Kim H (2012) Aggregation of human eyelid adipose-derived stem cells by human body fluids. Dev Reprod 16:339-351. https://doi.org/10.12717/DR.2012.16.4.339
  26. Taniguchi Ishikawa E, Chang KH, Nayak R, Olsson HA, Ficker AM, Dunn SK, Madhu MN, Sengupta A, Whitsett JA, Grimes HL, Cancelas JA (2013) Klf5 controls bone marrow homing of stem cells and progenitors through Rab5-mediated $\beta$1/$\beta$2-integrin trafficking. Nat Commun 4:1660. https://doi.org/10.1038/ncomms2645
  27. Tateishi K, Ando W, Higuchi C, Hart DA, Hashimoto J, Nakata K, Yoshikawa H, Nakamura N (2008) Comparison of human serum with fetal bovine serum for expansion and differentiation of human synovial MSC: potential feasibility for clinical applications. Cell Transplant 17:549-557. https://doi.org/10.3727/096368908785096024
  28. Uccelli A, Moretta L, Pistoia V (2008) Mesenchymal stem cells in health and disease. Nat Rev Immunology 8:726-736. https://doi.org/10.1038/nri2395
  29. Zhang H, Eisenried A, Zimmermann W, Shively JE (2013) Role of CEACAM1 and CEACAM20 in an in vitro model of prostate morphogenesis. PLoS One 8:e53359. https://doi.org/10.1371/journal.pone.0053359