Development and Reproduction (한국발생생물학회지:발생과생식)

The Korean Society of Developmental Biology (한국발생생물학회)
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$PKC{\eta}$ Regulates the $TGF{\beta}3$-induced Chondrogenic Differentiation of Human Mesenchymal Stem Cell

  • Ku, Bo Mi (Department of Anatomy & Neurobiology, Institute of Health Sciences, School of Medicine, Gyeongsang National University) ;
  • Yune, Young Phil (Department of Anatomy & Neurobiology, Institute of Health Sciences, School of Medicine, Gyeongsang National University) ;
  • Lee, Eun Shin (Department of Rehabilitation Medicine, School of Medicine, Gyeongsang National University) ;
  • Hah, Young-Sool (Clinical Research Institute, Gyeongsang National University Hospital) ;
  • Park, Jae Yong (Dept. of Physiology, School of Medicine, Gyeongsang National University) ;
  • Jeong, Joo Yeon (Department of Anatomy & Neurobiology, Institute of Health Sciences, School of Medicine, Gyeongsang National University) ;
  • Lee, Dong Hoon (Department of Anatomy & Neurobiology, Institute of Health Sciences, School of Medicine, Gyeongsang National University) ;
  • Cho, Gyeong Jae (Department of Anatomy & Neurobiology, Institute of Health Sciences, School of Medicine, Gyeongsang National University) ;
  • Choi, Wan Sung (Department of Anatomy & Neurobiology, Institute of Health Sciences, School of Medicine, Gyeongsang National University) ;
  • Kang, Sang Soo (Department of Anatomy & Neurobiology, Institute of Health Sciences, School of Medicine, Gyeongsang National University)
  • Received : 2013.10.18
  • Accepted : 2013.11.11
  • Published : 2013.12.31
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Abstract

Transforming growth factor (TGF) family is well known to induce the chondrogenic differentiation of mesenchymal stem cells (MSC). However, the precise signal transduction pathways and underlying factors are not well known. Thus the present study aims to evaluate the possible role of C2 domain in the chondrogenic differentiation of human mesenchymal stem cells. To this end, 145 C2 domains in the adenovirus were individually transfected to hMSC, and morphological changes were examined. Among 145 C2 domains, C2 domain of protein kinase C eta ($PKC{\eta}$) was selected as a possible chondrogenic differentiation factor for hMSC. To confirm this possibility, we treated $TGF{\beta}3$, a well known chondrogenic differentiation factor of hMSC, and examined the increased-expression of glycosaminoglycan (GAG), collagen type II (COL II) as well as $PKC{\eta}$ using PT-PCR, immunocytochemistry and Western blot analysis. To further evaluation of C2 domain of $PKC{\eta}$, we examined morphological changes, expressions of GAG and COL II after transfection of $PKC{\eta}$-C2 domain in hMSC. Overexpression of $PKC{\eta}$-C2 domain induced morphological change and increased GAG and COL II expressions. The present results demonstrate that $PKC{\eta}$ involves in the TGF-${\beta}3$-induced chondrogenic differentiation of hMSC, and C2 domain of $PKC{\eta}$ has important role in this process.

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References

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