Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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No Detection of Xenotropic Murine Leukemia Virus-Related Viruses in Prostate Cancer in Sanandaj, West of Iran

  • Khodabandehloo, Mazaher (Department of Microbiology, Faculty of Medicine, Kurdistan University of Medical Sciences) ;
  • Hosseini, Weria (Faculty of Allied Medical Sciences, Kurdistan University of Medical Sciences) ;
  • Rahmani, Mohammad-Reza (Department of Immunology, Faculty of Medicine, Kurdistan University of Medical Sciences) ;
  • Rezaee, Mohammad-Ali (Department of Immunology, Faculty of Medicine, Kurdistan University of Medical Sciences) ;
  • Hakhamaneshi, Mohammad-Saied (Cellular and Molecular Research Center, Kurdistan University of Medical Sciences) ;
  • Nikkhoo, Bahram (Department of Pathology, Faculty of Medicine, Kurdistan University of Medical Sciences) ;
  • Jalili, Ali (Cellular and Molecular Research Center, Kurdistan University of Medical Sciences)
  • Published : 2013.11.30
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Abstract

Background: Multiple etiologies have been hypothesized for prostate cancer, including genetic defects and infectious agents. A recently reported gamaretrovirus, xenotropic murine leukemia virus-related virus (XMRV) has been reported to be detected in prostate cancer. However, this virus has not been detected in similar groups of patients in other studies. Herein, we sought to detect XMRV in prostate cancers and benign controls in Sanandaj, west of Iran. Materials and Methods: In a case-control study, genomic DNA was extracted from formalin fixed and paraffin embedded prostate tissues from a total of 163 Iranian patients. We developed a conventional and a nested PCR assay using primers targeting to an env specific sequence of XMRV. PCR assays were carried out on 63 prostate cancers and 100 benign prostate hyperplasias. Results: Beta-actin sequences were successfully detected in the DNA extracts from all prostate tissues, confirming DNA extraction integrity. We did not detect XMRV in samples either from prostate cancers or benign prostate hyperplasias using XMRV specific primers. Conclusions: We conclude that in our population XMRV does not play a role in genesis of prostate cancer.

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References

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