Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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Glutathione S-Transferase Expression in Upper Urinary Tract Urothelial Carcinomas: a Taiwan Study

  • Chen, Szu-Han (Department of Urology, Kaohsiung Medical University Hospital, Kaohsiung Medical University) ;
  • Wu, Wen-Jeng (Department of Urology, Kaohsiung Medical University Hospital, Kaohsiung Medical University) ;
  • Tu, Hung-Pin (Department of Public Health and Environmental Medicine, School of Medicine, College of Medicine, Kaohsiung Medical University) ;
  • Li, Wei-Ming (Department of Urology, Kaohsiung Medical University Hospital, Kaohsiung Medical University) ;
  • Huang, Chun-Nung (Department of Urology, Kaohsiung Medical University Hospital, Kaohsiung Medical University) ;
  • Li, Ching-Chia (Department of Urology, Kaohsiung Medical University Hospital, Kaohsiung Medical University) ;
  • Lin, Hui-Hui (Department of Urology, Kaohsiung Medical University Hospital, Kaohsiung Medical University) ;
  • Ke, Hung-Lung (Department of Urology, Kaohsiung Medical University Hospital, Kaohsiung Medical University)
  • Published : 2013.11.30
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Abstract

Objectives: Glutathione S-transferase (GST) isoenzymes play important roles in resistance to cell apoptosis and carcinogenesis. We aimed to establish the relationship between GST expression and the prognosis of upper urinary tract urothelial carcinoma (UTT-UC) in Taiwan. Methods: This study retrospectively reviewed 46 patients with pathologically confirmed UUT-UC at Kaohsiung Medical University Hospital. In each patient, expression of GSTT1 and GSTP1 was compared between urothelial carcinoma and normal urothelial cells by Western blotting. Results: GSTP1 expression in the UUT-UC cells was significantly higher than that in normal urothelial cells (1.6 fold, p<0.001). Expression of GSTT1 was significantly associated with the invasiveness of the carcinoma (p=0.006). Conclusions: In UUT-UC, GSTP1 might be a potential tumor marker, whereas high GSTT1 expression could be used as an indicator of cancer progression. This study is the first to demonstrate potential applications of different GST isoenzymes for biomolecular analysis of UUT-UCs in Taiwan.

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References

  1. Berendsen CL, Peters WH, Scheffer PG, et al (1997). Glutathione S-transferase activity and subunit composition in transitional cell cancer and mucosa of the human bladder. Urology, 49, 644-51. https://doi.org/10.1016/S0090-4295(96)00575-4
  2. Cohen SM, Shirai T, Steineck G. (2000). Epidemiology and Etiology of Premalignant and Malignant Urothelial Changes. Scand J Urol Nephrol, 34, 105-15. https://doi.org/10.1080/00365590050509869
  3. Catto JW, Yates DR, Rehman I, et al (2007). Behavior of urothelial carcinoma with respect to anatomical location. J Urol, 177, 1715-20. https://doi.org/10.1016/j.juro.2007.01.030
  4. Epstein JI, Amin MB, Reuter VR, et al (1998). The World Health Organization/International Society of Urological Pathology consensus classification of urothelial (transitional cell) neoplasms of the urinary bladder. Bladder Consensus Conference Committee. Am J Surg Pathol, 22, 1435-48. https://doi.org/10.1097/00000478-199812000-00001
  5. Giralt M, Lafuente A, Pujol F, et al (1993). Enhanced glutathione S-transferase activity and glutathione content in human bladder cancer. Followup study: influence of smoking. J Urol, 149, 1452-4. https://doi.org/10.1016/S0022-5347(17)36413-3
  6. Greene FL, Page DL, Fleming ID, et al (2002). AJCC Cancer Staging Manual. 6th ed. Springer-Verlag, New York.
  7. Hurst R, Bao Y, Jemth P, et al (1998). Phospholipid hydroperoxide glutathione peroxidase activity of human glutathione transferases. Biochem J, 332, 97-100. https://doi.org/10.1042/bj3320097
  8. Hayes JD, Strange RC (2000). Glutathione S-transferase polymorphisms and their biological consequences. Pharmacology, 61, 154-66. https://doi.org/10.1159/000028396
  9. Kempkes M, Golka K, Reich S, et al (1996). Glutathione S-transferase GSTM1 and GSTT1 null genotypes as potential risk factors for urothelial cancer of the bladder. Arch Toxicol, 71, 123-6. https://doi.org/10.1007/s002040050366
  10. Karam JA, Lotan Y, Karakiewicz PI, et al (2007). Use of combined apoptosis biomarkers for prediction of bladder cancer recurrence and mortality after radical cystectomy. Lancet Oncol, 8, 128-36. https://doi.org/10.1016/S1470-2045(07)70002-5
  11. Ke HL, Wei YC, Yang SF, et al (2008). Overexpression of hypoxia-inducible factor-1alpha predicts an unfavorable outcome in urothelial carcinoma of the upper urinary tract. Int J Urol, 15, 200-5. https://doi.org/10.1111/j.1442-2042.2007.01978.x
  12. Ke HL, Chang LL, Yang SF, et al (2011). Osteopontin overexpression predicts poor prognosis of upper urinary tract urothelial carcinoma. Urol Oncol, 29, 703-9. https://doi.org/10.1016/j.urolonc.2009.10.009
  13. Ke HL, Tu HP, Lin HH, et al (2012). Cyclooxygenase-2 (COX-2) up-regulation is a prognostic marker for poor clinical outcome of upper tract urothelial cancer. Anticancer Res, 32, 4111-6.
  14. Lowe SW, Lin AW (2000). Apoptosis in cancer. Carcinogenesis. 21, 485-95. https://doi.org/10.1093/carcin/21.3.485
  15. Matic M, Simic T, Dragicevic D, et al (2010). Isoenzyme profile of glutathione transferases in transitional cell carcinoma of upper urinary tract. Transl Res, 155, 256-62. https://doi.org/10.1016/j.trsl.2009.11.001
  16. Oguztuzun S, Sezgin Y, Yazici S, et al (2011). Expression of glutathione-S-transferases isoenzymes and p53 in exfoliated human bladder cancer cells. Urol Oncol, 29, 538-44. https://doi.org/10.1016/j.urolonc.2009.08.001
  17. Pavanello S, Mastrangelo G, Placidi D, et al (2010). CYP1A2 polymorphisms, occupational and environmental exposures and risk of bladder cancer. Eur J Epidemiol, 25, 491-500. https://doi.org/10.1007/s10654-010-9479-8
  18. Pljesa-Ercegovac M, Savic-Radojevic A, Kravic-Stevovic T, et al (2010). Co-localization of GSTP1 and JNK in transitional cell carcinoma of urinary bladder. Genet Mol Biol, 33, 460-2. https://doi.org/10.1590/S1415-47572010005000063
  19. Pljesa-Ercegovac M, Savic-Radojevic A, Dragicevic D, et al (2011). Enhanced GSTP1 expression in transitional cell carcinoma of urinary bladder is associated with altered apoptotic pathways. Urol Oncol, 29, 70-7 https://doi.org/10.1016/j.urolonc.2008.10.019
  20. Simic T, Mimic-Oka J, Savic-Radojevic A, et al (2005). Glutathione S-transferase T1-1 activity upregulated in transitional cell carcinoma of urinary bladder. Urology, 65, 1035-40. https://doi.org/10.1016/j.urology.2005.01.005
  21. Savic-Radojevic A, Mimic-Oka J, Pljesa-Ercegovac M, et al (2007). Glutathione S-transferase-P1 expression correlates with increased antioxidant capacity in transitional cell carcinoma of the urinary bladder. Eur Urol, 52, 470-7. https://doi.org/10.1016/j.eururo.2007.01.046
  22. Simic T, Savic-Radojevic A, Pljesa-Ercegovac M, et al (2009). Glutathione S-transferases in kidney and urinary bladder tumors. Nat Rev Urol, 6, 281-9. https://doi.org/10.1038/nrurol.2009.49
  23. Townsend D, Tew K (2003). Cancer drugs, genetic variation and the glutathione-S-transferase gene family. Am J Pharmacogenomics, 3, 157-72. https://doi.org/10.2165/00129785-200303030-00002
  24. Wang T, Arifoglu P, Ronai Z, et al (2001). Glutathione S-transferase P1.1 (GSTP1.1) inhibits c-Jun N-terminal kinase (JNK1) signaling through interaction with the C terminus. J Biol Chem, 276, 20999-1003. https://doi.org/10.1074/jbc.M101355200
  25. Yang CR, Ou YC, Kuo JH, et al (1997). Intracellular glutathione content of urothelial cancer in correlation to chemotherapy response. Cancer Lett, 119, 157-62. https://doi.org/10.1016/S0304-3835(97)00274-7
  26. Yang MH, Chen KK, Yen CC, et al (2002). Unusually high incidence of upper urinary tract urothelial carcinoma in Taiwan. Urology, 59, 681-7. https://doi.org/10.1016/S0090-4295(02)01529-7
  27. Yanamadala V, Negoro H, Gunaratnam L, et al (2007). G$\alpha$12 stimulates apoptosis in epithelial cells through JNK1-mediated Bcl-2 degradation and up-regulation of IκBα. J Biol Chem, 282, 24352-63. https://doi.org/10.1074/jbc.M702804200
  28. Yeh HC, Huang CH, Yang SF, et al (2010). Nuclear factor-kappaB activation predicts an unfavourable outcome in human upper urinary tract urothelial carcinoma. BJU Int, 106, 1223-9. https://doi.org/10.1111/j.1464-410X.2010.09210.x

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