Asian Pacific Journal of Cancer Prevention

  • 제14권11호
  • /
  • Pages.6305-6309
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  • 2013
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  • 1513-7368(pISSN)
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  • 2476-762X(eISSN)
아시아태평양암예방학회 (Asian Pacific Journal of Cancer Prevention)
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TIAM2 Enhances Non-small Cell Lung Cancer Cell Invasion and Motility

  • Zhao, Zheng-Yuan (Department of General Thoracic Surgery, Affiliated Hospital of Logistics University of Chinese People's Armed Police Forces) ;
  • Han, Chen-Guang (Department of General Thoracic Surgery, Affiliated Hospital of Logistics University of Chinese People's Armed Police Forces) ;
  • Liu, Jun-Tao (Department of General Thoracic Surgery, Affiliated Hospital of Logistics University of Chinese People's Armed Police Forces) ;
  • Wang, Chang-Lei (Department of General Thoracic Surgery, Affiliated Hospital of Logistics University of Chinese People's Armed Police Forces) ;
  • Wang, Yi (Department of General Thoracic Surgery, Affiliated Hospital of Logistics University of Chinese People's Armed Police Forces) ;
  • Cheng, Li-Ya (Department of General Thoracic Surgery, Affiliated Hospital of Logistics University of Chinese People's Armed Police Forces)
  • 발행 : 2013.11.30
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초록

Background: TIAM2, a Rac guanine nucleotide exchange factor, is closely associated with cell adherence and migration. Here, we aimed to investigate the role of TIAM2 in non-small cell lung cancer (NSCLC) cells. Materials and Methods: A small interference RNA (siRNA) was introduced to silence the expression of TIAM2. Invasion and motility assays were then performed to assess the invasion and motility potential of NSCLC cells. GST-pull down assays were used to detect activation of Rac1. Results: TIAM2 was highly expressed in NSCLC cells. Knockdown of TIAM2 inhibited the invasion and motility, and suppressed activation of Rac1. Further experiments demonstrated that knockdown of TIAM2 could up-regulate the expression of E-cadherin, and down-regulate the expression of MMP-3, Twist and Snail. Conclusions: Our data suggest that TIAM2 can promote invasion and motility of NSCLC cells. Activation of Rac1 and regulation of some EMT/invasion-related genes may be involved in the underlying processes.

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  2. Sp1-mediated ectopic expression of T-cell lymphoma invasion and metastasis 2 in hepatocellular carcinoma vol.5, pp.3, 2016, https://doi.org/10.1002/cam4.611
  3. Enhancer profiling identifies critical cancer genes and characterizes cell identity in adult T-cell leukemia vol.130, pp.21, 2017, https://doi.org/10.1182/blood-2017-06-792184
  4. STEF/TIAM2-mediated Rac1 activity at the nuclear envelope regulates the perinuclear actin cap vol.9, pp.1, 2018, https://doi.org/10.1038/s41467-018-04404-4
  5. Two isoforms of the RAC-specific guanine nucleotide exchange factor TIAM2 act oppositely on transmission ratio distortion by the mouse t-haplotype vol.15, pp.2, 2019, https://doi.org/10.1371/journal.pgen.1007964