Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Quality Characteristics and Biogenic Amine Production of Makgeolli Brewed with Commercial Nuruks

시판 누룩으로 제조한 막걸리의 품질특성과 biogenic amine 생성

  • Jeong, Seok-Tae (Fermentation & Food Science Division, National Academy of Agricultural Science) ;
  • Kwak, Hee-Jung (Nutrition and Functional Food Research Team, National Institute of Food and Drug Safety Evaluation) ;
  • Kim, Soon-Mi (Department of Food & Nutrition, Gachon University)
  • 정석태 (국립농업과학원 발효식품과) ;
  • 곽희정 (식품의약품안전처 식품위해평가부 영양기능연구팀) ;
  • 김순미 (가천대학교 생활과학대학 식품영양학과)
  • Received : 2013.08.06
  • Accepted : 2013.10.02
  • Published : 2013.12.31
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Abstract

Makgeolli mashes that were brewed using five different commercial nuruks (fermentation starters) were investigated for changes in physicochemistry, microbial diversity, and biogenic amine (BA) production. Mash A brewed with the nuruk (Gaeryang-nuruk) had the highest level of alcohol concentration and the greatest number of yeast cells, whereas mash E had the greatest number of bacterial cells. Only three biogenic amines were detected in the makgeolli mashes: tyramine, putrescine, and cadaverine. Using a PCR-DGGE technique, we observed that mash E had the highest BA production, and had the greatest number of bands on the denaturing gradient gels. We also observed that the numbers of bacterial cells correlated significantly with the putrescine and the total BA content, and that the BA content correlated significantly with the color values (L, a, b). This study shows that the quality of a makgeolli can depend on the type of nuruk. Therefore, we suggest that the quality management of makgeolli should start with the stage of nuruk manufacture.

본 연구는 국내 약 탁주 제조업체에서 사용하는 대표적인 개량누룩 1종과 재래누룩 4종을 선발하여 막걸리를 담고, 담금 과정 중의 누룩에 따른 술덧의 이화학적, 미생물학적 변화와 BA생성 특성 및 이들의 상관관계를 조사하고자 하였다. 발효 초기 술덧의 pH는 4.40-5.00이었으나 4일째 감소한 후 이후 약간 증가하는 경향을 나타내었다. 발효 7일째의 총 산 함량은 시험구 간의 차이가 크게 나타나 누룩 C가 가장 많았으며, 개량누룩을 사용한 누룩 A가 가장 낮았다. 술덧의 당도는 누룩 D가 가장 낮았으며, 누룩 A는 발효 초기부터 7일까지 가장 높은 당도를 유지하였다. 발효 7일째 술덧의 색도를 측정한 결과 밝기는 누룩 A가 가장 높고, 누룩 E가 가장 낮았으나 적색과 황색은 이와 반대되는 결과를 보여주었다. 술덧의 알코올 농도는 누룩 A가 가장 많았으며, 누룩 B, 누룩 C, 누룩 E, 누룩 D의 순이었으나, 휘발산은 알코올 농도가 가장 많았던 누룩 A의 함량이 가장 낮았다. 술덧의 효모 수와 총 세균 수는 발효 1일에 비해 7일에 감소하였다. 발효 7일째 효모 수는 가장 높은 알코올 농도를 나타낸 누룩 A가 가장 많았고, 총 세균 수는 누룩 E가 가장 많았다. 술덧에서 검출된 BA는 총 3종으로 putrescine은 5종의 술덧 모두에서 발효 초기부터 검출되었으며, tyramine은 누룩 C, 누룩 D, 누룩 E의 발효 7일째에서만 검출되었고, cadaverine은 누룩 E에서만 발효 1일째부터 검출되었다. 이들 술덧의 미생물 균주의 변화를 PCR-DGGE 기법으로 살펴본 결과 발효 1일과 7일째 BA 생성량이 가장 많았던 누룩 E의 밴드가 가장 다양해지는 것을 확인할 수 있었다. 위의 이러한 특성 간의 상관관계를 분석한 결과 총 세균수는 putrescine 및 총 BA 함량과 정의 상관관계를 나타내었고, BA와 색도(L, a, b값) 역시 유의적인 상관관계가 있음을 알 수 있었다. 본 연구는 막걸리의 품질 지표로 활용될 수 있는 BA의 생성이 누룩의 종류에 따라 다르다는 것과 함께 막걸리의 품질 관리는 누룩의 제조 단계에서부터 시작되어야 함을 시사하고 있다.

Keywords

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