Korean Chemical Engineering Research

  • 제51권1호
  • /
  • Pages.151-156
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  • 2013
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  • 0304-128X(pISSN)
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  • 2233-9558(eISSN)
한국화학공학회 (The Korean Institute of Chemical Engineers)
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미세유체 결정화기를 이용한 탄산칼슘 Biomineralization

CaCO3 Biomineralization in Microfluidic Crystallizer

  • Seo, Seung Woo (Department of Chemical Engineering, Chungnam National University) ;
  • Ko, Kwan Young (Department of Chemical Engineering, Chungnam National University) ;
  • Lee, Chang Soo (Department of Chemical Engineering, Chungnam National University) ;
  • Kim, In Ho (Department of Chemical Engineering, Chungnam National University)
  • 투고 : 2012.10.17
  • 심사 : 2012.11.20
  • 발행 : 2013.02.01
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초록

Polydimethylsiloxane (PDMS) 기반의 미세유체 시스템을 이용해 탄산칼슘의 결정화 실험을 수행하였다. 탄산칼슘의 결정화를 위한 다양한 반응방법 중 액체-액체 반응을 위해 염화칼슘 수용액과 탄산나트륨 수용액을 사용하였고 아스파르트산을 첨가하여 탄산칼슘 결정 중 베터라이트와 칼사이트의 생성에 어떤 차이를 보이는지 조사하였다. 그리고 탄산칼슘의 결정화 진행상황에서 결정핵 생성에 유리한 염화칼슘과 탄산나트륨의 비율을 조사하였다. 이를 위해 크리스마스트리 모양의 미세유체 반응기를 사용하여 채널 내부에 염화칼슘과 탄산나트륨의 농도구배를 형성하도록 하였다. 미세유체 결정화기 내부를 광학현미경으로 촬영한 결과, 탄산나트륨과 염화칼슘의 농도비가 2:1일 때 결정핵이 생성됨을 확인하였고 핵 생성 이후의 결정 성장 과정을 촬영하여 결정형태의 변화를 관찰하였다. 아스파르트산의 첨가 시에 결정핵 생성과 성장을 저해하며 전체 결정형태 중 베터라이트의 비율이 높아짐을 보였다.

Crystallization of $CaCO_3$ is practiced on a polymethylsiloxane (PDMS) - based microfluidic system. Liquid- liquid reaction was investigated by mixing calcium chloride ($CaCl_2$) and sodium carbonate ($Na_2CO_3$) solution to crystallize $CaCO_3$. Aspartic acid (Asp) was added to investigate the morphology change such as vaterite and calcite. Suitable ratio of $Na_2CO_3$ and $CaCl_2$ was searched for initial seed formation. Christmas tree model was used as microfluidic device to form concentration gradient of $Na_2CO_3$ and $CaCl_2$. After observing microfluidic channel by using optical microscope, we found that seeds of $CaCO_3$ were formed under the condition that the ratio of $Na_2CO_3$ and $CaCl_2$ was 2:1. Morphology of crystals were also observed as $CaCO_3$ crystals grow. When Asp was added, vaterite crystal was more frequently found in two morphologies (vaterite and calcite) and seed formation and crystal growth were inhibited.

키워드

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피인용 문헌

  1. Lysozyme Crystallization in Droplet-based Microfluidic Device vol.51, pp.6, 2013, https://doi.org/10.9713/kcer.2013.51.6.760
  2. Microfluidic Control of Nucleation and Growth of CaCO3 vol.18, pp.8, 2018, https://doi.org/10.1021/acs.cgd.8b00508