Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Effects of Residual Hypochlorite Ion on Methane Production during the Initial Anaerobic Digestion Stage of Pig Slurry

  • Received : 2012.09.18
  • Accepted : 2012.10.31
  • Published : 2013.01.01
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Abstract

The hypochlorite ion ($OCl^-$) is a widely used disinfecting agent in pig rearing in Korea, but its residual effect on $CH_4$ production from pig slurry is unclear. The objective of this study was to investigate the inhibition effects of residual $OCl^-$ on $CH_4$ production during the initial anaerobic digestion stage of pig slurry. Three organic concentrations (9.9, 26.2 and 43.7 g/L) of volatile solids (VS) were tested with the addition of 52.3 mg/L $OCl^-$, ten times of the typical concentration used in Korea, or without $OCl^-$ (Control) in anaerobic batch culture. The culture was run under mesophilic ($38^{\circ}C$) conditions for 20 d. At the lowest organic concentration with $OCl^-$, the VS degradation was 10.3% lower (p<0.05) than Control, while at the higher organic concentration with $OCl^-$, it did not differ from Control. $CH_4$ yields were higher in the control treatments than their $OCl^-$ counterpart cultures, and $CH_4$ yields of Control and $OCl^-$ treatments at the organic concentrations of 9.9, 26.2 and 43.7 g/L differed in the probability level (p) of 0.31, 0.04, and 0.06, respectively. Additionally, $CH_4$ concentration increased steeply and reached 70.0% within 4 d in the absence $OCl^-$, but a gradual increase up to 60.0% was observed in 6 d in the $OCl^-$ treated cultures. The $R_m$ (the maximum specific $CH_4$ production rate) and ${\lambda}$ (lag phase time) of 9.9 g/L with $OCl^-$ were 8.1 ml/d and 25.6 d, while the $R_m$ was increased to 15.1 ml/d, and ${\lambda}$ was reduced to 11.4 d in PS-III (higher organic concentration) with $OCl^-$. The results suggest that a prolonged fermentation time was necessary for the methanogens to overcome the initial $OCl^-$ inhibitory effect, and an anaerobic reactor operated with high organic loadings was more advantageous to mitigate the inhibitory effect of residual hypochlorite ion.

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References

  1. Albrich, J. M., C. A. McCarthy and J. K. Hurst. 1981. Biological reactivity of hypochlorous acid: Implications for microbiocidal mechanisms of leukocyte myelperoxidase. Proc. Natl. Acad. Sci. USA. 78:210-214. https://doi.org/10.1073/pnas.78.1.210
  2. APHA. 1998. Standard methods for the examination of water and wastewater. 20th edn. American Public Health Association, Washington, DC, USA.
  3. Battersby, N. S. and V. Wilson. 1989. Survey of the anaerobic biodegradation potential of organic chemicals in digesting sludge. Appl. Environ. Microbiol. 55:433-439.
  4. Bessems, E. 1998. The effect of practical conditions on the efficiency of disinfectants. Int. Biodeterior. Biodegradation 41:177-183. https://doi.org/10.1016/S0964-8305(98)00022-5
  5. Beuvink, J. M., S. F. Spoelstra and R. J. Hogendrop. 1992. An automated method for measuring the time course of gas production of feedstuffs incubated with buffered rumen fluid. Netherlands J. Agric. Sci. 40:401-407.
  6. Chmielewski, R. A. N. and J. F. Frank. 2003. Biofilm formation and control in food processing facilities. Compr. Rev. Food Sci. Food Saf. 2:22-32. https://doi.org/10.1111/j.1541-4337.2003.tb00012.x
  7. Ducan, S. and T. Daniele. 1996. Hypochlorous acid stress in Escherichia coli: Rresistance, DNA damage, and comparison with hydrogen peroxide stress. J. Bacteriol. 178:6145-6150.
  8. Garcia, M. T., E. Campos, J. Sanchez-Leal and I. Ribosa. 1999. Effect of the alkyl chain length on the anaerobic biodegradability and toxicity of quaternary ammonium based surfactants. Chemosphere 38:3473-3483. https://doi.org/10.1016/S0045-6535(98)00576-1
  9. Kitis, M. 2004. Disinfection of wastewater with peracetic acid: A review. Environ. Int. 30:47-55. https://doi.org/10.1016/S0160-4120(03)00147-8
  10. KME. 1999. The discharging unit of livestock wastes. Notification number 1999-109. Korean Ministry of Environment, Seoul, Korea.
  11. KMFAFF. 2008. The criteria concerned with livestock breeding density. Notification number 2008-79. Korean Ministry of Food, Agriculture, Forestry and Fisheries, Seoul, Korea.
  12. Lambert, R. J. W. and M. D. Johnston. 2001. The effect of interfering substances on the disinfection process: a mathematic model. J. Appl. Microbiol. 91:548-555. https://doi.org/10.1046/j.1365-2672.2001.01422.x
  13. Leyer, G. L. and A. E. Johnson. 1997. Acid adaptation sensitizes Salmonella typhimurium to hypochlorous acid. Appl. Environ. Microbiol. 63:461-467.
  14. Maillard, J. Y. 2002. Bacterial target sites for biocide action. J. Appl. Microbiol (Suppl.). 92:16S-27S. https://doi.org/10.1046/j.1365-2672.92.5s1.3.x
  15. McDonnell, G. and A. D. Russell. 1999. Antiseptics and disinfectants: activity, action, and resistance. Clin. Microbiol. Rev. 12:147-179.
  16. McKenna, S. M. and K. J. A. Davies. 1988. Bacterial killing by phagocytes: Potential role(s) of hypochlorous acid and hydrogen peroxide in protein turnover, DNA synthesis, and RNA synthesis. Basic Life. Sci. 49:829-832.
  17. Metcalf, E. 2002. Wastewater Engineering: Treatment and Reuse. 4thh edn. McGraw-Hill, New York, USA.
  18. Rakita, R. M., B. R. Michel and H. Rosen. 1990. Differential inactivation of Escherichia coli membrane dehydrogenases by a myeloperoxidase-mediated antimicrobial system. Biochemstery 29:1075-1080. https://doi.org/10.1021/bi00456a033
  19. Shih, K. L. and J. Lederberg. 1976. Effects of chloramine on Bacillus subtilis deoxyribonucleic acid. J. Bacteriol. 125:934-945.
  20. Tezel, U., J. A. Pierson and S. G. Pavlostathis. 2006. Fate and effect of quaternary ammonium compounds on a mixed methanogenic culture. Water Res. 40:3660-3668. https://doi.org/10.1016/j.watres.2006.06.019
  21. Williams, A., M. Amat-Marco and M. D. Collins. 1996. Pylogenetic analysis of Butyrivibrio strains reveals three distinct groups of species within the Clostridium subphylum of gram-positive bacteria. Int. J. Syst. Evol. Microbiol. 46:195-199.
  22. Yu, Y., C. Lee and S. Hwang. 2005. Analysis of Community structures in anaerobic processes using a quantitative real-time PCR method. Water Sci. Technol. 52:85-91.

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