Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Effects of ascorbic acid, citric acid and silver nitrate on the growth of in vitro lily plantlets and reduction of browning

Ascorbic acid, citric acid 및 AgNO3가 나리 기내식물체 생장촉진 및 갈변화 감소에 미치는 영향

  • Roh, Hee Sun (Department of Biotechnology, College of Biomedical & Health Sciences, Glocal Campus, Konkuk university) ;
  • Lee, Sang Il (Department of Biotechnology, College of Biomedical & Health Sciences, Glocal Campus, Konkuk university) ;
  • Kang, Yun Im (Division of Floriculture, National Institute of Horticultural & Herbal Science) ;
  • Kim, Mi Seon (Division of Floriculture, National Institute of Horticultural & Herbal Science) ;
  • Kim, Jong Bo (Department of Biotechnology, College of Biomedical & Health Sciences, Glocal Campus, Konkuk university)
  • 노희선 (건국대학교 의료생명대학 생명공학과) ;
  • 이상일 (건국대학교 의료생명대학 생명공학과) ;
  • 강윤임 (농촌진흥청 국립원예특작과학원 원예작물부 화훼과) ;
  • 김미선 (농촌진흥청 국립원예특작과학원 원예작물부 화훼과) ;
  • 김종보 (건국대학교 의료생명대학 생명공학과)
  • Received : 2013.12.23
  • Accepted : 2013.12.26
  • Published : 2013.12.31
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Abstract

Lily is an important cut-flower in Korea and world as well due to it's a variety of flower colors and various sizes of flowers. To develop elite lily cultivars, conventional breeding techniques have been used so far. However, an introduction of tissue culture system in mass propagation of bulbs and regeneration of shoots with a high efficiency is prerequisite at this moment. Especially, growth of bulbs and shoots as well as reduction of browning is critical factor to proliferate bulbs with shoots of superior lines or cultivar in lily. For this purpose, we tried to test whether ascorbic acid, citric acid and silver nitrate in medium to facilitate growth of bulbs and shoots as well as reduction of browning of bulb scales in lily. As a result, ascorbic acid and silver nitrate showed no significant differences compared to control plants in the growth of bulbs and shoots. When bulb scales were treated with $150mg{\cdot}l^{-1}$ of citric acid, formations of shoots and bulbs showed best result. While, bulb scale treated with $100mg{\cdot}l^{-1}$ of citric acid showed the growth of shoot and root as well as increasing of fresh weight compared to other treatments. Regarding the reduction of browning, $150mg{\cdot}l^{-1}$ of ascorbic acid showed the best result with the less than 2%. Although more experiments with several commercial varieties are needed in the future to establish mass propagation of bulbs of lily, results obtained in this study are supposed to provide the basic knowledge and contribution in tissue culture system of lily.

본 연구 결과 질산은과 구연산 및 비타민 C의 처리는 레드플레임의 갈변화 방지에는 효과적이나, 레드플레임 인편의 재분화 및 생장에 있어 비타민 C와 질산은의 처리는 효과적 이지 못하였다. 반면 구연산의 경우 레드플레임의 재분화 및 생장에 도움을 주는 것으로 나타났으나 $150mg{\cdot}l^{-1}$이상의 고농도에서는 생육이 저해됨을 보여 $100mg{\cdot}l^{-1}$ 처리가 나리의 재분화 및 생장에 가장 효율적임을 확인하였다. 또한 절편체의 갈변화를 감소시키는 부분에서는 비타민 C $150mg{\cdot}l^{-1}$ 처리가 1.5%의 가장 낮은 갈변율을 나타내어 갈변화 현상 감소에 가장 적합한 처리구로 판단되었다. 본 실험결과는 향후 나리기내 식물체생산에 문제점으로 발생하는 갈변화 현상을 감소시키고 인편 재분화 및 생장을 촉진시켜 우량 나리 기내 묘의 대량증식체계 확립을 통한 우량종구묘 생산체계 확립에 기여하여 농가 소득 향상에 도움이 될 것으로 판단된다.

Keywords

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