Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
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CMV-P1 Resistance Evaluation Using Enzyme-linked Immunosorbent Assay of Pepper Genetic Sources (Capsicum spp.)

Enzyme-linked Immunosorbent Assay를 이용한 CMV-P1 저항성 고추 유전자원 평가

  • Shin, JiEun (Department of Plant Science, Gangneung-Wonju National University) ;
  • Xu, Sheng Jun (Department of Plant Science, Gangneung-Wonju National University) ;
  • Kim, Jun Young (Department of Plant Science, Gangneung-Wonju National University) ;
  • Woo, JeHyeon (Department of Plant Science, Gangneung-Wonju National University) ;
  • Kim, Han Gil (Department of Plant Science, Gangneung-Wonju National University) ;
  • Park, Yong Ju (Biotong Co.) ;
  • Hong, Sae Jin (Department of Plant Science, Gangneung-Wonju National University) ;
  • Kim, Byung Sup (Department of Plant Science, Gangneung-Wonju National University)
  • 신지은 (강릉원주대학교 식물생명과학과) ;
  • 서생군 (강릉원주대학교 식물생명과학과) ;
  • 김준영 (강릉원주대학교 식물생명과학과) ;
  • 우제현 (강릉원주대학교 식물생명과학과) ;
  • 김한길 (강릉원주대학교 식물생명과학과) ;
  • 박용주 (바이오통) ;
  • 홍세진 (강릉원주대학교 식물생명과학과) ;
  • 김병섭 (강릉원주대학교 식물생명과학과)
  • Received : 2013.01.30
  • Accepted : 2013.07.31
  • Published : 2013.12.31
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Abstract

Recently, Cucumber mosaic virus (CMV)-P1 infection in pepper cultivation is very serious problem, which causes low marketability and yield. In this research, 56 domestic pepper cultivars including 20 PR (Phytophthora resistance) cultivars, 31 foreign pepper lines collected from USA and 112 genetic pepper resources form RDA Genebank were used for CMV-P1 resistance evaluation. Resistance evaluation was performed at 24 days and 51 days after artificial inoculation of peppers by enzyme-linked immunosorbent assay (ELISA). Among the 36 domestic cultivars 'Muhanjilju' were resistant to CMV-P1. All 20 PR cultivars and 29 foreign peppers except 'NuMex Twilight' and 'Chainese Giant' were susceptible. Among 112 pepper lines from RDA Genebank, nine pepper lines were resistant, and 17 pepper lines were moderately resistant, and 86 pepper lines were susceptible. Almost all domestic peppers on the market were highly susceptible to CMV-P1, whereas 17.2% of foreign pepper cultivars and genetic pepper lines from RDA Genebank were resistant or moderately resistant. Resistant pepper lines selected in this study can be used as genetic sources for breeding CMV-P1 resistant pepper.

최근 고추재배에 있어 CMV-P1감염은 상품성 저하와 생산량 감수를 발생시키는 매우 심각한 문제이다. 본 실험에서는 역병 저항성 품종인 PR계통을 포함한 국내 시판 56품종과 미국에서 도입해 온 31품종 도입자원 및 농촌진흥청 유전자원센터(RDA Genebank)로부터 받은 112개의 고추 유전자원을 CMV-P1 저항성 검정에 사용하였다. 저항성 검정은 인공 접종 후 24일 후와 51일 후 ELISA를 실시하였다. 36개 시판품종 중 '무한질주' 품종이 CMV-P1에 저항성이었다. 모든 PR품종과 중도저항성인 'NuMex Twilight'와 'Chainese Giant'를 제외한 29개 외국 도입고추는 감수성이었다. RDA Genebank의 112계통의 고추 유전자원에서는 저항성 계통이 9, 중도저항성을 나타내는 계통이 17, 나머지 86계통은 모두 감수성으로 조사되었다. 국내 시판 고추품종은 거의 CMV-P1에 높은 감수성을 보이는 것으로 확인되는 반면, 외국 도입자원과 유전자원의 17.2%가 저항성이거나 중도저항성으로 확인되었다. 본 연구 결과로 확인된 저항성 고추는 CMV-P1 저항성 고추육종에 유용한 유전자원으로 이용될 수 있다.

Keywords

References

  1. Agrios, G.N. 2005. Plant pathology: Serology of plant viruses. Elsevier Academic Press, New York. p. 744-747.
  2. Cai, W.Q., R.X. Fang, H.S. Shang, X. Wang, F.L. Zhang, Y.R. Li, J.C. Zhang, X.Y. Cheng, G.L. Wang, and K.Q. Mang. 2003. Development of CMV-and TMV-resistance transgenic chili pepper: Field performance and biosafety assessment. Mol. Breed. 11:25-35. https://doi.org/10.1023/A:1022655204552
  3. Caranta, C., A. Palloix, V. Lefebvre, and A.M. Daubeze. 1997. QTLs for a component of partial resistance to Cucumber mosaic virus in pepper: Restriction of virus installation in host-cells. Theor. Appl. Genet. 94:431-438. https://doi.org/10.1007/s001220050433
  4. Caranta, C., S. Pflieger, V. Lefebvre, A.M. Daubeze, A. Thabuis, and A. Palloix. 2002. QTLs involved in the restriction of Cucumber mosaic virus (CMV) long-distance movement in pepper. Theor. Appl. Genet. 104:586-591. https://doi.org/10.1007/s001220100753
  5. Chaim, A.B., R.C. Grube, M. Lapidot, and M. Jahn. 2001. Identification of quantitative trait loci associated with resistance to Cucumber mosaic virus in Capsicum annuum. Theor. Appl. Genet. 102:1213-1220. https://doi.org/10.1007/s001220100581
  6. Chen, Z.L., H. Gu, Y. Li, Y. Su, P. Wu, Z. Jiang, X. Ming, J. Tian, N. Pan, and L.J. Qu. 2003. Safety assessment for genetically modified sweet pepper and tomato. Toxicology 188:297-307. https://doi.org/10.1016/S0300-483X(03)00111-2
  7. Cho, J.D., J.S. Kim, J.H. Lee, and B.N. Chung. 2008. Characteristics of a NP strain for Cucumber mosaic virus (CMV-NP) identified newly from sweet pepper showing fruit necrosis. Res. Plant Dis. 14:134-137. https://doi.org/10.5423/RPD.2008.14.2.134
  8. Cho, J.D., S.H. Lee, J.S. Kim, G.S. Choi, H.R. Kim, B.N Chung, and K.H. Ryu. 2006a. Characteristics of Cucumber mosaic virus-VCH causing vein chlorosis on red pepper on Korea. Res. Plant. Dis. 12:226-230. https://doi.org/10.5423/RPD.2006.12.3.226
  9. Cho, J.D., S.H. Lee, G.S. Choi, T.J. Lim, B.N. Chung, K.H. Ryu, and J.S. Kim. 2006b. Viruses and their mixed infections on red peppers at Jeolla-do province in 2005. J. Plant Pathol. 21:124-125. (Abstr.)
  10. Choi, G.S., J.H. Kim, D.H. Lee, J.S. Kim, and K.H. Ryu. 2005. Occurrence and distribution of viruses infecting pepper in Korea. Plant Pathol. J. 21:258-261. https://doi.org/10.5423/PPJ.2005.21.3.258
  11. Choi, G.S., J.H. Kim, K.H. Ryu, J.K. Choi, S.Y. Chae, H.R. Kim, B.N. Chung, J.S. Kim, and Y.M. Choi. 2002. First report of Tobacco mild green mosaic virus infection pepper in Korea. Plant Pathol. J. 18:323-327. https://doi.org/10.5423/PPJ.2002.18.6.323
  12. Choi, J.K., E.J. Hong, J.Y. Lee, and M.U. Chang. 1995. Responses to infection of Tobacco mosaic viruspepper strain (TMV-P) in transgenic tobacco plants expressing the TMV-P coat protein or its antisense RNA. Kor. J. Plant Pathol. 11:374-379.
  13. Francki, R.I.B., D.W. Mossop, and T. Hatta. 1979. Cucumber mosaic virus. CMI/AAB Descriptions of Plant Viruses No. 213.
  14. Grube, R.C., Y. Zhang, J.F. Murphy, F. Loaiza-Figueroa, V.K. Lackney, R. Provvidenti, and M.K. Jahn. 2000. New sources of resistance to Cucumber mosaic virus in Capsicum frutescens. Plant Dis. 84:885-981. https://doi.org/10.1094/PDIS.2000.84.8.885
  15. Han, B.K., S.W. Kang, and G.P. Lee. 2011. Case study of methodological procedure for assessment of introduced gene in genetically modified plants: CMV resistant red pepper. Kor. J. Hort. Sci. Technol. 29(Suppl. II): 196. (Abstr.)
  16. Im, K.H., B.K. Chung, J.Y. Yoon, and S.K. Green. 1991. A Survey on viruses infecting peppers (Capsicum annum) in Korea by microplate method of enzyme-linked immunesorvent assay (ELISA). Kor. J. Plant Pathol. 7:252-256.
  17. Kang, G.Y., J.I. Choi, and Y.J. Na. 1973. Isolation and identification of viruses affecting pepper (Capsicum annuum L) in Korea. Kor. J. Hort. Sci. Technol. 13:35-43.
  18. Kang, G.Y. and J.I. Choi. 1976. Studies on the resistance of some varietics of pepper to Tobacco mosaic virus. Kor. J. Hort. Sci. Technol. 17:61-68.
  19. Kang, S.W., B.K. Han, and G.P. Lee. 2011. Case study of methodological procedure for stability assessment of introduced gene in genetically modified plant: CMV resistant red pepper. Kor. J. Hort. Sci. Technol. 29(Suppl. II): 196. (Abstr.)
  20. Kang, W.H., N.H. Hoang, H.B. Yang, J.K. Kwon, S.H. Jo, J.K. Seo, K.H. Kim, D. Choi, and B.C. Kang. 2010. Molecular mapping and characterization of a single dominant gene controlling CMV resistance in peppers (Capsicum annuum L.) Theor. Appl. Genet. 120:1587-1596. https://doi.org/10.1007/s00122-010-1278-9
  21. Kim, J.S., S.K. Kim, G.S. Choi, and M.W. Lee. 1990a. Virus disease incidence and symptom appearance in red pepper. Kor. J. Plant Pathol. 6:125-132.
  22. Kim, J.S., S.K. Kim, S.Y. Lee, and M.W. Lee. 1990b. A pepper vein chlorosis virus causing stem necrosis and vein chlorosis on red pepper in Korea. Kor. J. Plant Pathol. 6:376-381.
  23. Kim, J.S. and K.H. Lee. 1991. Virus disease incidence and symptom expression of peppers cultivated in vinyl house, and serological relationship of CMV isolates from peppers. Res. Pept. RDA (C.P) 33:8-14.
  24. Kim, J.S. and G.S. Choi. 2001. Viruses infecting peppers in the world and their characteristics (introduction). J. Kor. Capsicum Res. Coop. 7:23-48.
  25. Kim, J.H. 2011. Comparison of field adaptability and growth characteristics of CMV-tolerant GM pepper (Capsicum annuum L.). Ph.D. Thesis. Korea Univ., Seoul, Korea.
  26. Kim, J.H., G.S. Choi, and J.K. Choi. 2002. Characterization of Cucumber mosaic virus subgroup II isolated from paprika in Korea. Plant Pathol. J. 18:6-11. https://doi.org/10.5423/PPJ.2002.18.1.006
  27. Korean Statistical Information Service (KOSIS). 2012. Pepper of cultivation area. http://www.kosis.kr.
  28. Lapidot, M., R. Ben-Joseph, S. Ben-Harush, M. Pilowsky, S. Cohen, and C. Shifriss. 1997. Tolerance to Cucumber mosaic virus in pepper: Development of advanced breeding lines and evaluation of virus level. Plant Dis. 81:185-188. https://doi.org/10.1094/PDIS.1997.81.2.185
  29. Lee, S.H., S.M. Kim, H.S. Choi, J.W. Park, T.S. Jin, and K.W. Lee. 2002. Pepper mild mottle virus is a major Tobamovirus on pepper in Korea. Plant Pathol. J. 18:381. (Abstr.)
  30. Lee, Y.H., M. Jung, S.H. Shin, J.H. Lee, S.H. Choi, N.H. Her, J.H. Lee, K.H. Ryu, K.Y. Park, and C.H. Harn. 2009. Transgenic peppers that are highly tolerant to a new CMV pathotype. Plant Cell Rep. 28:223-232. https://doi.org/10.1007/s00299-008-0637-3
  31. Lim, P.O., J.S. Ryu, H. Lee, Y.S. Park, J.M. Kwak, J.K. Choi, and H.G. Na. 1997. Resistance to Tobamoviruses in transgenic tobacco plants expressing the coat protein gene of Pepper mild mottle virus (Korean isolate). Mol. Cells. J. 30:313-319.
  32. Mumford, R.A., I. Barker, and K.R. Wood. 1994. The detection of Tomato spotted wilt virus using the polymerase chain reaction. J. Virol. Methods 46:303-311. https://doi.org/10.1016/0166-0934(94)90002-7
  33. Nongwoobio Products information. 2013. Products information. http://www. nongwoobio.co.kr.
  34. Palukaitis, P., M.J. Roossinck, R.G. Dietzgen, and R.I.B. Francki. 1992. Cucumber mosaic virus. Adv. Virus Re. 41:281-348. https://doi.org/10.1016/S0065-3527(08)60039-1
  35. Ryu, K.H. 2007. Development of transgenic resistant pepper plant to multivirus infection with virus and host derived genetic resources. Seoul Women's University. Res. paper. p. 9.
  36. Suzuki, K., T. Kuroda, Y. Miura, and J. Murai. 2003. Screening and field trials of virus resistant sources in Capsicum spp. Plant Dis. 87:779-783. https://doi.org/10.1094/PDIS.2003.87.7.779
  37. Sung, J.H., J.H. Park, H.Y. Shin, M.U. Chang, H. Sayama, and H. Atarashi. 2002. Protective effect of two Japanese attenuated Cucumber mosaic virus (CMV) strains, PV1-K and PV2-K against a Korean virulent CMV isolate, CMV-KP in Korea hot pepper cultivars. Plant Pathol. J. 18:373. (Abstr.)
  38. Yang, H.B., W.Y. Liu, W.H. Kang, M. Jahn, and B.C. Kang. 2009. Development of SNP markers linked to the L locus in Capsicum spp. by a comparative genetic analysis. Mol. Breed 24:433-446. https://doi.org/10.1007/s11032-009-9304-9

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