Pediatric Infection and Vaccine

  • 제20권3호
  • /
  • Pages.123-130
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  • 2013
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  • 2384-1079(pISSN)
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  • 2384-1087(eISSN)
대한소아감염학회 (The Korean Society of Pediatric Infectious Diseases)
권호 표지

PD-1 유전자 제거 마우스에서 홍역바이러스 감염

Measles Viral Infection in PD-1 Gene Knockout Mice

  • 전진경 (연세대학교 원주의과대학 소아과학교실) ;
  • 김규연 (연세대학교 의과대학 소아과학교실) ;
  • 허지애 (연세대학교 의과대학 소아과학교실) ;
  • 강동원 (관동대학교 의과대학 약리학교실) ;
  • 김기환 (연세대학교 의과대학 소아과학교실) ;
  • 김동수 (연세대학교 의과대학 소아과학교실)
  • Chun, Jin Kyong (Department of Pediatrics, Yonsei University College of Medicine) ;
  • Kim, Kyu Yeun (Department of Pediatrics, Yonsei University College of Medicine) ;
  • Hur, Ji Ae (Department of Pediatrics, Yonsei University College of Medicine) ;
  • Kang, Dong Won (Department of Pharmacology, Kwandong University College of Medicine) ;
  • Kim, Ki Hwan (Department of Pediatrics, Yonsei University College of Medicine) ;
  • Kim, Dong Soo (Department of Pediatrics, Yonsei University College of Medicine)
  • 투고 : 2013.08.19
  • 심사 : 2013.10.13
  • 발행 : 2013.12.25
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초록

목적: 아급성경화범뇌염은 퇴행성 신경질환으로 홍역바이러스의 지속적 중추신경계 감염으로 나타난다. 저자들은 마우스 모델을 통해 아급성경화범뇌염 발병에서 만성 바이러스 감염에 관여하는 PD-1 유전자의 역할을 알아보고자 하였다. 방법: 3주령의 동형 PD-1 유전자 제거 마우스, 이형 PD-1 유전자 제거 마우스, 야생 BALB/c 마우스를 대상으로 측뇌실내 홍역바이러스를 주입하여 동물 모델로 하였다. 바이러스 주입 3개월 후, 마우스의 뇌 조직학적 소견을 관찰하고 혈청을 분리하여 IL-21의 혈청 농도를 ELISA kit을 통해 측정하였다. 결과: 야생 BALB/c 마우스에서 가장 많은 국소적 뇌백질의 괴사 및 성상세포의 증가가 관찰되었다. 이형 마우스에서 뇌실질의 병변은 적었으며 동형 마우스는 거의 보이지 않았다. 세 그룹에서 모두 혈청 IL-21의 증가는 보이지 않았다. 결론: 이 결과는 PD-1 유전자가 만성 바이러스 감염에 중요한 역할을 함을 시사한다.

Purpose: Subacute sclerosing panencephalitis (SSPE) is a neurodegerative disease due to persistent measles virus infection. We investigated the role of programmed death-1 (PD-1) molecule which is related with chronic viral infection in developing SSPE in mouse. Methods: We adopt the $PD-1^{-/-}$, $PD-1^{-/+}$, and wild type BALB/c 3 week old mice to make an animal model of SSPE by injecting measles virus (SSPE strain) intraventricularly. Three months after infusion of virus, the mice were sacrificed and examined if the typical pathologic lesions had been progressed. The sera were collected from each group of mice and the serum level of IL-21 was measured with ELISA kit. Results: The necrotic lesions on white matter and gliosis were found in focal areas in wild type BALB/c. The extent of lesion was smaller in heterotype BALB/c. Scanty lesions were found in $PD-1^{-/-}$ mice. The sera level of IL-21 was not elevated in all three groups. Conclusion: Our data suggest that the PD-1 molecule may play a role in persistent viral infection.

키워드

참고문헌

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