International Journal of Industrial Entomology and Biomaterials

  • 제27권2호
  • /
  • Pages.329-332
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  • 2013
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  • 3022-7542(pISSN)
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  • 2586-4785(eISSN)
한국잠사학회 (Korean Society of Sericultural Science)
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Activity and Stability of Immobilized Enzyme on Silk Sericin Bead

  • Oh, Hanjin (National Instrumentation Center for Environmental Management, Seoul National University) ;
  • Lee, Ki Hoon (Department of Biosystems & Biomaterials Science and Engineering, Seoul National University)
  • 투고 : 2013.12.10
  • 심사 : 2013.12.17
  • 발행 : 2013.12.31
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초록

In present preliminary report, we showed the possibility of silk sericin (SS) in enzyme immobilization. SS beads were prepared and enzymes were immobilized on it. The specific activity of immobilized a-chymotrypsin retained more than 87% compared to the free enzyme. The immobilized a-chymotrypsin has better stability against ethanol especially those immobilized on SS beads coagulated in methanol. Immobilized trypsin and lipase had also comparable apparent activity compared to free enzyme. Our result indicates that SS could be a good candidate for enzyme immobilization support due to its hydrophilicity.

키워드

참고문헌

  1. Aramwit P, Siritientong T, Srichana T (2012) Potential applications of silk sericin, a natural protein from textile industry by-products. Waste Manage Res 30, 217-224. https://doi.org/10.1177/0734242X11404733
  2. Asakura T, Yoshimizu H, Kuzuhara A, Matsunaga T (1988) Mechanism of glucose oxidase immobilization with silk fibroin. J Seri Sci Jpn 57, 203-209.
  3. Kuzuhara K, Asakura T, Tomoda R, Matsunaga T (1987) Use of silk fibroin for enzyme membrane. J Biotech 5, 199-207.
  4. Kwak HW, Yang YS, Kim MK, Lee JY, Yun H, Kim MH, Lee KH (2013) Chromium(VI) adsorption behavior of silk sericin beads. Int J Indust Entomol 26, 47-53. https://doi.org/10.7852/ijie.2013.26.1.047
  5. Lee KH, Kang GD, Shin BS, Park YH, Nahm JH (2004) Immobilization of trypsin onto silk fibroin fiber via spacer arms. Int J Indust Entomol 8, 195-200.
  6. Oh HJ, Lee JY, Kim A, Ki CS, Kim JW, Park YH, Lee KH (2007a) Preparation of silk sericin seads using LiCl/DMSO solvent and their potential as a drug carrier for oral administration. Fiber Polym 8, 470-476. https://doi.org/10.1007/BF02875867
  7. Oh HJ, Lee JY, Kim A, Lee KH, Shin BS (2008) Preparation of PVA/ silk sericin blend fiber using LiCl/DMSO solvent. Text Sci Eng 45, 1-5.
  8. Oh HJ, Lee JY, Lee YK, Lee KH (2007b) Enhanced mechanical property of silk sericin beads prepared from ethanol-precipitated sericin. Int J Indust Entomol 15, 171-174.
  9. Secundo F (2013) Conformational changes of enzymes upon immobilization. Chem Soc Rev 42, 6250-6261. https://doi.org/10.1039/c3cs35495d
  10. Yun H, Kim MK, Kwak HW, Lee JY, Kim MH, Kim EH, Lee KH (2013) Preparation and characterization of silk sericin/glycerol/graphene oxide nanocomposite film. Fiber Polym 14, 2111-2116. https://doi.org/10.1007/s12221-013-2111-2
  11. Zhang YQ (2002) Applications of natural silk protein sericin in biomaterials. Biotechnol Adv 20, 91-100. https://doi.org/10.1016/S0734-9750(02)00003-4

피인용 문헌

  1. Preparation and Characterization of Silk Beads for Protein Delivery System vol.28, pp.2, 2014, https://doi.org/10.7852/ijie.2014.28.2.66
  2. Catalytic Activity of Immobilized Chymotrypsin on Hybrid Silica-Magnetic Biocompatible Particles and Its Application in Peptide Synthesis vol.190, pp.4, 2020, https://doi.org/10.1007/s12010-019-03158-z