DOI QR코드

DOI QR Code

Effects of Fungicides on Inhibition of in Vitro Strawberry Pollen Germination

In Vitro에서 살균제의 딸기 화분발아 억제 효과

  • Nam, Myeong Hyeon (Nonsan Strawberry Experiment Station, Chungcheongnam-do Agricultural Research & Extension Services) ;
  • Kim, Hyun Sook (Nonsan Strawberry Experiment Station, Chungcheongnam-do Agricultural Research & Extension Services) ;
  • Choi, Je Hyun (Nonsan Strawberry Experiment Station, Chungcheongnam-do Agricultural Research & Extension Services) ;
  • Lee, He Duck (Nonsan Strawberry Experiment Station, Chungcheongnam-do Agricultural Research & Extension Services)
  • 남명현 (충청남도농업기술원 논산딸기시험장) ;
  • 김현숙 (충청남도농업기술원 논산딸기시험장) ;
  • 최재현 (충청남도농업기술원 논산딸기시험장) ;
  • 이희덕 (충청남도농업기술원 논산딸기시험장)
  • Received : 2012.12.19
  • Accepted : 2013.06.16
  • Published : 2013.09.30

Abstract

Fungicide applications are required to prevent the strawberry from Botrytis fruit rot and powdery mildew that infect open strawberry flowers, however, their effects of fungicides on pollen germination of strawberry have been rarely documented, particularly those from recently developed active fungicidal ingredients. In this study we have evaluated the effects of 24 commercial fungicidal formulations and 6 organic materials on pollen germination in 3 strawberry cultivars using in vitro assays. Pollens from strawberry had higher germination rates on agar with sucrose of 18% and $25^{\circ}C$ than other tested conditions. Pollen germination rates of cvs. Seolhyang, Maehyang, and Kumhyang at 18% sucrose and $25^{\circ}C$ were 15.3, 18.4 and 30.7%, respectively. Pyraclostrobin, azoxystrobin, kresoxim-methyl, dichlofluanid, iminoctadine tris, and sulfur showed the strongest inhibitory efficacy with the germination rates of more than 93.8% compared to the no-fungicide control. Germination was not significantly affected by simeconazole and procymidone. This in vitro germination study may provide information useful for selecting fungicides in flowering stage to strawberry farmers.

딸기의 잿빛곰팡이병과 흰가루병을 방제하기 위해 개화기에 살균제 처리가 요구된다. 최근에 딸기에 등록된 살균제들이 딸기 화분 발아에 미치는 영향은 거의 알려지지 않았다. 따라서 본 연구에서는 in vitro 상에서 딸기 3품종의 화분에 대한 24종의 살균제와 6종의 친환경자재에 대해 발아에 미치는 영향을 조사하였다. 딸기의 화분은 18% sucrose agar 배지에서 $25^{\circ}C$로 처리되었을 때 쉽게 발아되었으며, '설향', '매향', '금향'의 평균 화분 발아율은 각각 15.3, 18.4, 30.7%을 보였다. Pyraclostrobin, azoxystrobin, kresoxim-methyl, dichlofluanid, iminoctadine tris, sulfur는 무처리구 대비 93.8% 이상의 화분 발아 억제가를 나타내었다. 반면, simeconazole과 procymidone 살균제의 화분 발아율은 영향이 가장 적었다. 이 in vitro 검정 결과는 딸기 재배농가의 개화기 살균제 선정에 대한 정보를 제공할 수 있을 것이다.

Keywords

References

  1. Bartlett, D.W., J.M. Clough, J.R. Godwin, A.A. Hall, M. Hamer, and B. Parr-Dobrzanski. 2002. The strobilurin fungicides. Pest Manage. Sci. 58:649-662. https://doi.org/10.1002/ps.520
  2. Bristow, P.R. and G.E. Windom. 1987. Effects of selected fungicides, insecticides, and adjuvants on in vitro germination of highbush blueberry pollen. Plant Dis. 71:326-328. https://doi.org/10.1094/PD-71-0326
  3. Cali, I.O. 2009. The effect of fungicide application on pollen structure in tomato (Lycopersicon esculentum Mill.) plant. J. Appl. Biological Sci. 3:37-40.
  4. Church, R.M. and R.R. Williams. 1977. The toxicity to apple pollen of several fungicides, as demonstrated by in vivo and in vitro techniques. J. Hort. Sci. 52:429-436.
  5. Eaton, G.W. and L.I. Chen. 1969. The effect of captan on strawberry pollen germination. J. Amer. Soc. Hort. Sci. 94:558-560.
  6. Fungicide Resistance Action Committee (FRAC). 2012. FRAC code list 2012: Fungicide sorted by mode of action (including RFAC code numbering). Http://www.frac.info.
  7. He, Y., H.Y. Wetzstein, and B.A. Palevitz. 1995. The effects of a triazole fungicide, propiconazole, on pollen germination, tube growth and cytoskeletal distribution in Tradescantia virginiana. Sex Plant Reprod. 8:210-216.
  8. Holb, I.J. 2008. Influence of pesticide use on flower formation and fertility of some fruit species. Intl. J. Hort. Sci. 14:103-106.
  9. Johannsson, M.H. and A.G. Stephenson. 1998. Effects of temperature during microsporogenesis on pollon performance in Cucurbita pepo L. (Cucurbitaceae). Intl. J. Plant Sci. 159:616-626. https://doi.org/10.1086/297580
  10. Kang, S.S., Y.K. Kim, S.B. Jeong, K.S. Cho, J.J. Choi, J.W. Han, and H.J. Lee. 2010. Effects of pesticides on pear pollen germination and pollen tube elongation. Kor. J. Hort. Sci. Technol. 28:209-215.
  11. Kargar, M.H. and A. Imani. 2011. Effects of fungicides on pollen germination peach and nectarine in vitro. Afr. J. Plant Sci. 5:643-647.
  12. Koyuncu, F. 2006. Response of in vitro pollen germination and pollen tube growth of strawberry cultivars to temperature. Europ. J. Hort. Sci. 71:125-128.
  13. Ledesma, N. and N. Sugiyama. 2005. Pollen quality and performance in strawberry plants exposed to high-temperature stress. J. Amer. Soc. Hort. Sci. 130:341-347.
  14. Mayer, D.F. and J.D. Lunden. 1986. Toxicity of fungicides and an acaricide to honey bees (Hymenopteta: Apidae) and their effects on bee foraging behavior and pollen viability on blooming apples and pears. Environ. Entomol. 15:1047-1049. https://doi.org/10.1093/ee/15.5.1047
  15. Ministry for Food, Agriculture, Forestry and Fisheries (MFAFF). 2012. 2011 production amount and index of agriculture and forestry. http://www.maf.go.kr.
  16. Nam, M.H., H.S. Kim, W.K. Lee, M.L. Gleason, and H.G. Kim. 2011. Control efficacy of gray mold on strawberry fruits by timing of chemical and microbial fungicide applications. Kor. J. Hort. Sci. Technol. 29:151-155.
  17. Voyiatzisi, D.G. and G. Paraskevopoulou-Paroussi. 2002. Factors affecting the quality and in vitro germination capacity of strawberry pollen. J. Hort. Sci. Biotechnol. 77:200-203.
  18. Wetzstein, H.Y. 1990. Stigmatic surface degeneration and inhibition of pollen germination with selected pesticidal sprays during receptivity in pecan. J. Amer. Soc. Hort. Sci. 115:656-661.
  19. Yi, W., S.E. Law, and H.Y. Wetzstein. 2003a. An in vitro study of fungicide effects on pollen germination and tube growth in almond. HortScience 38:1086-1088.
  20. Yi, W., S.E. Law, and H.Y. Wetzstein. 2003b. Fungicide sprays can injure the stigmatic surface during receptivity in almond flowers. Ann. Bot. 91:335-341. https://doi.org/10.1093/aob/mcg019
  21. Yoder, K., R. Yuan, L. Combs, R. Byers, J. McFerson, and T. Schmidt. 2009. Effects of temperature and the combination of liquid lime sulfur and fish oil on pollen germination, pollen tube growth, and fruit set in apples. HortScience 44:1277-1283.
  22. Ypema, H.L. and R.E. Gold. 1999. Kresoxim-methyl: Modification of a naturally occurring compound to produce a new fungicide. Plant Dis. 83:4-19. https://doi.org/10.1094/PDIS.1999.83.1.4
  23. Zarrabi, A. and A. Imani. 2011. Effects of fungicides on in-vitro pollen germination, tube growth and morphology of almond (Prunus dulcis). Afr. J. Agri. Res. 6:5645-5649.

Cited by

  1. 신고배 개화기 NaDCC 처리에 의한 검은별무늬병의 방제 vol.22, pp.2, 2013, https://doi.org/10.11625/kjoa.2014.22.2.347