Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Comparative Differential Expressions of Porcine Satellite Cell during Adipogenesis, Myogenesis, and Osteoblastogenesis

  • Jeong, Jin Young (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Jang Mi (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration) ;
  • Rajesh, Ramanna Valmiki (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration) ;
  • Suresh, Sekar (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration) ;
  • Jang, Gul Won (Division of Planning and Coordination, National Institute of Animal Science, Rural Development Administration) ;
  • Lee, Kyung-Tai (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Tae Hun (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration) ;
  • Park, Mina (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration) ;
  • Jeong, Hak Jae (Division of Animal Biotechnology, National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Kyung Woon (Division of Animal Biotechnology, National Institute of Animal Science, Rural Development Administration) ;
  • Cho, Yong Min (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration) ;
  • Lee, Hyun-Jeong (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration)
  • Received : 2013.12.03
  • Accepted : 2013.12.17
  • Published : 2013.12.31
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Abstract

Satellite cells were derived from muscular tissue in postnatal pig. Satellite cell is an important to growth and development in animal tissues or organs. However, the progress underlying induced differentiation is not clear. The aim of this study was to evaluate the morphologic and the transcriptome changes in porcine satellite cell (PSC) treated with insulin, rosiglitazone, or dexamethasone respectively. PSC was obtained from postnatal muscle tissue. In study 1, for study the effect of insulin and FBS on the differentiated satellite cells, cells were cultured at absence or presence of insulin treated with FBS. Total RNA was extracted for determining the expression levels of myogenic PAX3, PAX7, Myf5, MyoD, and myogenin genes by real-time PCR. Myogenic genes decreased expression levels of mRNA in treated with insulin. In study 2, in order to clarify the relationship between rosiglitazone and lipid in differentiated satellite cells, we further examined the effect of FBS on lipid accumulation in the presence or absence of the rosiglitazone and lipid. Significant differences were observed between rosiglitazone and lipid by FBS. The mRNA of FABP4 and $PPAR{\gamma}$ increased in rosiglitazone treatment. In study 3, we examined the effect of dexamethasone on osteogenic differentiation in PSC. The mRNA was increased osteoblasotgenic ALP and ON genes treated with dexamethasone in 2% FBS. Dexamethasone induces osteoblastogenesis in differentiated PSC. Taken together, in differentiated PSCs, FABP4 and $PPAR{\gamma}$ increased to rosiglitazone. Whereas, no differences to FBS and lipid. These results were not comparable with previous reports. Our results suggest that adipogenic, myogenic, and osteoblastogenic could be isolated from porcine skeletal muscle, and identify culture conditions which optimize proliferation and differentiation formation of PSC.

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References

  1. Akune T, Ohba S, Kamekura S, Yamaguchi M, Chung UI, Kubota N, Terauchi Y, Harada Y, Azuma Y, Nakamura K, Kadowaki T, Kawaguchi H (2004): PPARgamma insufficiency enhances osteogenesis through osteoblast formation from bone marrow progenitors. Clin Invest 113:846-855. https://doi.org/10.1172/JCI200419900
  2. Alliston T, Choy L, Ducy P, Karsenty G, Derynck R (2001): TGF-beta-induced repression of CBFA1 by Smad3 decreases cbfa1 and osteocalcin expression and inhibits osteoblast differentiation. EMBO J 20:2254-2272. https://doi.org/10.1093/emboj/20.9.2254
  3. Asakura A, Komaki M, Rudnicki M (2001): Muscle satellite cells are multipotential stem cells that exhibit myogenic, osteogenic, and adipogenic differentiation. Differentiation 68:245-253. https://doi.org/10.1046/j.1432-0436.2001.680412.x
  4. Bajard L, Relaix F, Lagha M, Rocancourt D, Daubas P, Buckingham ME (2006): A novel genetic hierarchy functions during hypaxial myogenesis: Pax3 directly activates Myf5 in muscle progenitor cells in the limb. Genes Dev 20:2450-2464. https://doi.org/10.1101/gad.382806
  5. Chargé SB, Rudnicki MA (2004): Cellular and molecular regulation of muscle regeneration. Physiol Rev 84:209-238. https://doi.org/10.1152/physrev.00019.2003
  6. Doumit ME, Merkel RA (1992): Conditions for isolation and culture of porcine myogenic satellite cells. Tissue Cell 24:253-262. https://doi.org/10.1016/0040-8166(92)90098-R
  7. Du M, Huang Y, Das AK, Yang Q, Duarte MS, Dodson MV, Zhu MJ (2013): Meat Science and Muscle Biology Symposium: manipulating mesenchymal progenitor cell differentiation to optimize performance and carcass value of beef cattle. J Anim Sci 91:1419-1427. https://doi.org/10.2527/jas.2012-5670
  8. Goulding M, Lumsden A, Paquette AJ (1994): Regulation of Pax-3 expression in the dermomyotome and its role in muscle development. Development 120:957-971.
  9. Grimaldi PA, Teboul L, Inadera H, Gaillard D, Amri EZ (1997): Trans-differentiation of myoblasts to adipoblasts: triggering effects of fatty acids and thiazolidinediones. Prostaglandins Leukot Essent Fatty Acids 57:71-75. https://doi.org/10.1016/S0952-3278(97)90495-6
  10. Hamm JK, Park BH, Farmer SR (2001): A role for C/EBP beta in regulating peroxisome proliferator-activated receptor gamma activity during adipogenesis in 3T3-L1 preadipocytes. J Biol Chem 276: 18464-18471. https://doi.org/10.1074/jbc.M100797200
  11. Livak KJ, Schmittgen TD (2001): Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25:402-408. https://doi.org/10.1006/meth.2001.1262
  12. Luppen CA, Smith E, Spevak L, Boskey AL, Frenkel B (2003): Bone morphogenetic protein-2 restores mineralization in glucocorticoid-inhibited MC3T3-E1 osteoblast cultures. J Bone Miner Res 18:1186-1197. https://doi.org/10.1359/jbmr.2003.18.7.1186
  13. Messina G, Cossu G (2009): The origin of embryo nic and fetal myoblasts: a role of Pax3 and Pax7. Genes Dev 23:902-905. https://doi.org/10.1101/gad.1797009
  14. Parker MH, Seale P, Rudnicki MA (2003): Looking back to the embryo: defining transcriptional networks in adult myogenesis. Nat Rev Genet 4:497-507.
  15. Rosen ED, Spiegelman BM (2002): Molecular regulation of adipogenesis. Annu Rev Cell Dev Biol 16: 145-171.
  16. Sabourin LA, Rudnicki MA (2000): The molecular regulation of myogenesis. Clin Genet 57:16-25.
  17. Saini A, Stewart CE (2006): Adult stem cells: the therapeutic potential of skeletal muscle. Curr Stem Cell Res Ther 1:157-171. https://doi.org/10.2174/157488806776956841
  18. Seale P, Sabourin LA, Girgis-Gabardo A, Mansouri A, Gruss P, Rudnicki MA (2000): Pax7 is required for the specification of myogenic satellite cells. Cell 102:777-786. https://doi.org/10.1016/S0092-8674(00)00066-0
  19. Shin DW, Kim SN, Lee SM, Lee W, Song MJ, Park SM, Lee TR, Baik JH, Kim HK, Hong JH, Noh M (2009): (-)-Catechin promotes adipocyte differentiation in human bone marrow mesenchymal stem cells through PPAR gamma transactivation. Biochem Pharmacol 77:125-133. https://doi.org/10.1016/j.bcp.2008.09.033
  20. Singh NK, Chae HS, Hwang IH, Yoo YM, Ahn CN, Lee SH, Lee HJ, Park HJ, Chung HY (2007): Transdifferentiation of porcine satellite cells to adipoblasts with ciglitizone. J Anim Sci 85:1126-1135. https://doi.org/10.2527/jas.2006-524
  21. Sirabella D, De Angelis L, Berghella L (2013): Sources for skeletal muscle repair: from satellite cells to reprogramming. J Cachexia Sarcopenia Muscle 4: 125-136. https://doi.org/10.1007/s13539-012-0098-y
  22. Taylor-Jones JM, McGehee RE, Rando TA, Lecka- Czernik B, Lipschitz DA, Peterson CA (2002): Activation of an adipogenic program in adult myoblasts with age. Mech Ageing Dev 123:649-661. https://doi.org/10.1016/S0047-6374(01)00411-0
  23. Vourc'h P, Romero-Ramos M, Chivatakarn O, Young HE, Lucas PA, El-Kalay M, Chesselet MF (2004): Isolation and characterization of cells with neurogenic potential from adult skeletal muscle. Biochem Biophys Res Commun 317:893-901. https://doi.org/10.1016/j.bbrc.2004.03.121
  24. Wu H, Ren Y, Li S, Wang W, Yuan J, Guo X, Liu D, Cang M (2012): In vitro culture and induced differentiation of sheep skeletal muscle satellite cells. Cell Biol Int 36:579-587. https://doi.org/10.1042/CBI20110487
  25. Yablonka-Reuveni Z, Rudnicki MA, Rivera AJ, Primig M, Anderson JE, Natanson P (1999): The transition from proliferation to differentiation is delayed in satellite cells from mice lacking MyoD. Dev Biol 210:440-455. https://doi.org/10.1006/dbio.1999.9284
  26. Yanik SC, Baker AH, Mann KK, Schlezinger JJ (2011): Organotins are potent activators of $PPAR{\gamma}$ and adipocyte differentiation in bone marrow multipotent mesenchymal stromal cells. Toxicol Sci 122:476-488. https://doi.org/10.1093/toxsci/kfr140
  27. Zhang JY, Yu SJ, Fu Y (2013): Effect of sonicated extracts of Porphyromonas gingivalis on osteogenic differentiation of mouse osteoblasts. Zhonghua Kou Qiang Yi Xue Za Zhi 48:398-402.