Reproductive and Developmental Biology

  • 제37권4호
  • /
  • Pages.219-224
  • /
  • 2013
  • /
  • 1738-2432(pISSN)
  • /
  • 2288-0151(eISSN)
한국동물번식학회 (The Korean Society of Animal Reproduction)
권호 표지
DOI QR코드

DOI QR Code

Comparison of Gene Expression Levels of Porcine Satellite Cells from Postnatal Muscle Tissue during Differentiation

  • Jeong, Jin Young (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Jang Mi (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration) ;
  • Rajesh, Ramanna Valmiki (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration) ;
  • Suresh, Sekar (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration) ;
  • Jang, Gul Won (Division of Planning and Coordination, National Institute of Animal Science, Rural Development Administration) ;
  • Lee, Kyung-Tai (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Tae Hun (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration) ;
  • Park, Mina (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration) ;
  • Jeong, Hak Jae (Division of Animal Biotechnology, National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Kyung Woon (Division of Animal Biotechnology, National Institute of Animal Science, Rural Development Administration) ;
  • Cho, Yong Min (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration) ;
  • Lee, Hyun-Jeong (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration)
  • 투고 : 2013.12.03
  • 심사 : 2013.12.17
  • 발행 : 2013.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Muscular satellite cell (SC), which is stem cell of postnatal pig, is an important for study of differentiation into adipogenesis, myogenesis, and osteoblastogenesis. In this study, we isolated and examined from pig muscle tissue to determine capacity in proliferate, differentiate, and expression of various genes. Porcine satellite cells (PSC) were isolated from semimembranosus (SM) muscles of 90~100 days old pigs according to standard conditions. The cell proliferation increased in multi-potent cell by Masson's, oil red O, and Alizarin red staining respectively. We performed the expression levels of differentiation related genes using real-time PCR. We found that the differentiation into adipocyte increased expression levels of both fatty acid binding protein 4 (FABP4) and peroxisome proliferator-activated receptor gamma ($PPAR{\gamma}$) genes (p<0.01). Myocyte increased the expression levels of the myosin heavy chain (MHC), myogenic factor 5 (Myf5), myogenic regulatory factor (MyoD), and Myogenic factor 4 (myogenin) (p<0.01). Osteoblast increased the expression levels of alkaline phosphatase (ALP) (p<0.01). Finally, porcine satellite cells were induced to differentiate towards adipogenic, myogenic, and osteoblastogenic lineages. Our results suggest that muscle satellite cell in porcine may influence cell fate. Understanding the progression of PSC may lead to improved strategies for augmenting meat quality.

키워드

참고문헌

  1. Asakura A, Komaki M, Rudnicki M (2001): Muscle satellite cells are multipotential stem cells that exhibit myogenic, osteogenic, and adipogenic differentiation. Differentiation 68:245-253. https://doi.org/10.1046/j.1432-0436.2001.680412.x
  2. Asada K, Obata K, Horiguchi K, Takaki M (2012): Age-related changes in afferent responses in sensory neurons to mechanical stimulation of osteoblasts in coculture system. Am J Physiol Cell Physiol 302: C757-765. https://doi.org/10.1152/ajpcell.00362.2011
  3. Bi W, Gu Z, Zheng Y, Zhang X, Guo J, Wu G (2013): Heterodimeric BMP-2/7 antagonizes the inhibition of all-trans retinoic acid and promotes the osteoblastogenesis. PLoS One 8: e78198. https://doi.org/10.1371/journal.pone.0078198
  4. Buckingham M, Montarras D (2008): Skeletal muscle stem cells. Curr Opin Genet Dev 18:330-336. https://doi.org/10.1016/j.gde.2008.06.005
  5. Chargé SB, Rudnicki MA (2004): Cellular and molecular regulation of muscle regeneration. Physiol Rev 84:209-238. https://doi.org/10.1152/physrev.00019.2003
  6. Doumit ME, Merkel RA (1992): Conditions for isolation and culture of porcine myogenic satellite cells. Tissue Cell 24:253-262. https://doi.org/10.1016/0040-8166(92)90098-R
  7. Guo C, Yuan L, Wang JG, Wang F, Yang XK, Zhang FH, Song JL, Ma XY, Cheng Q, Song GH (2013): Lipopolysaccharide (LPS) induces the apoptosis and inhibits osteoblast differentiation through JNK pathway in MC3T3-E1 cells. Inflammation Epub ahead of print.
  8. Hawke TJ, Garry DJ (2001): Myogenic satellite cells: physiology to molecular biology. J Appl Physiol 91: 534-551. https://doi.org/10.1152/jappl.2001.91.2.534
  9. Li X, Wang X, Zhang P, Zhu L, Zhao T, Liu S, Wu Y, Chen X, Fan M (2012): Extracellular signal-regulated kinase 1/2 mitogen-activated protein kinase pathway is involved in inhibition of myogenic differentiation of myoblasts by hypoxia. Exp Physiol 97:257-264. https://doi.org/10.1113/expphysiol.2011.061382
  10. Liu S, Wang Y, Wang L, Wang N, Li Y, Li H (2010): Transdifferentiation of fibroblasts into adipocyte-like cells by chicken adipogenic transcription factors. Comp Biochem Physiol A Mol Integr Physiol 156: 502-508. https://doi.org/10.1016/j.cbpa.2010.04.003
  11. Livak KJ, Schmittgen TD (2001): Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25:402-408. https://doi.org/10.1006/meth.2001.1262
  12. Salmerón C, Acerete L, Gutiérrez J, Navarro I, Capilla E (2013): Characterization and endocrine regulation of proliferation and differentiation of primary cultured preadipocytes from gilthead sea bream (Sparus aurata). Domest Anim Endocrinol 45:1-10. https://doi.org/10.1016/j.domaniend.2013.02.002
  13. Samulin J, Berget I, Lien S, Sundvold H (2008): Differential gene expression of fatty acid binding proteins during porcine adipogenesis. Comp Biochem Physiol B Biochem Mol Biol 151:147-152. https://doi.org/10.1016/j.cbpb.2008.06.010
  14. Scarda A, Franzin C, Milan G, Sanna M, Dal Prà C, Pagano C, Boldrin L, Piccoli M, Trevellin E, Granzotto M, Gamba P, Federspil G, De Coppi P, Vettor R (2010): Increased adipogenic conversion of muscle satellite cells in obese Zucker rats. Int J Obes (Lond) 34: 1319-1327. https://doi.org/10.1038/ijo.2010.47
  15. Sudo H, Kodama HA, Amagai Y, Yamamoto S, Kasai S (1983): In vitro differentiation and calcification in a new clonal osteogenic cell line derived from newborn mouse calvaria. J Cell Biol 96:191-198. https://doi.org/10.1083/jcb.96.1.191
  16. Wu X, Chim SM, Kuek V, Lim BS, Chow ST, Zhao J, Yang S, Rosen V, Tickner J, Xu J (2013): HtrA1 is upregulated during RANKL-induced osteoclastogenesis, and negatively regulates osteoblast differentiation and BMP2-induced Smad1/5/8, ERK and p38 phosphorylation. FEBS Lett Epub ahead of print.
  17. Xiang X, Zhao J, Xu G, Li Y, Zhang W (2011): m- TOR and the differentiation of mesenchymal stem cells. Acta Biochim Biophys Sin (Shanghai) 43:501-510. https://doi.org/10.1093/abbs/gmr041
  18. Yamanouchi K, Ban A, Shibata S, Hosoyama T, Murakami Y, Nishihara M (2007): Both PPARgamma and C/EBPalpha are sufficient to induce transdifferentiation of goat fetal myoblasts into adipocytes. J Reprod Dev 53:563-572. https://doi.org/10.1262/jrd.18169