DOI QR코드

DOI QR Code

The Effects of Antioxidants on the Culture of Mouse Preantral Follicles In Vitro

  • Kim, Dong-Hoon (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Kim, Dong-Kyo (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Yang, Byoung-Chul (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Park, Jin-Ki (Animal Biotechnology Division, National Institute of Animal Science, RDA)
  • 투고 : 2013.11.10
  • 심사 : 2013.11.19
  • 발행 : 2013.12.31

초록

In order to investigate the effects of antioxidants on the culture of mouse preantral follicles in vitro, we examined the effects of taurine, glutathione and catalase on their growth and maturation. Addition of taurine was not effective on the survival of preantral follicles. However, metaphase II rates of oocytes within preantral follicles were significantly higher in 1 mM treated group than in control and 10 mM treated group (p<0.05). Glutathione did not improved the rates of survival and metaphase II oocytes. However, metaphase II rates of oocytes progressively decreased with increasing glutathione concentration. Catalase also showed that the rates of survival and metaphase II oocytes progressively decreased with increasing concentration. Especially, all of preantral follicles cultured in medium containing 100 IU/ml catalase were degenerated. These results suggest that low concentraion of taurine, as an antioxidant, have positive effect on the culture of mouse preantral follicles in vitro.

키워드

참고문헌

  1. Batt PA, Gardner DK, Cameron AW (1991): Oxygen concentration and protein source affect the development of preimplantation goat embryos in vitro. Reprod Fertil Dev 3:601-607. https://doi.org/10.1071/RD9910601
  2. Cortvrindt R, Smitz J, Van Steirteghem AC (1996): In-vitro maturation, fertilization and embryo development of immature oocytes from early preantral follcles from prepuberal mice in a simplified culture system. Human Reprod 11:2656-2666. https://doi.org/10.1093/oxfordjournals.humrep.a019188
  3. Eppig JJ, Downs SM (1987): The effect of hypoxanthine on mouse oocyte growth and development in vitro; maintenance of meiotic arrest and gonadotrophin- induced oocyte maturation. Dev Biol 119:313-321. https://doi.org/10.1016/0012-1606(87)90037-6
  4. Eppig JJ, O'Brien MJ, Pendola FL, Watanabe S (1998): Factors affecting the developmental competence of mouse oocytes grown in vitro: follicle-stimulating hormone and insulin. Biol Reprod 59:1445-1453. https://doi.org/10.1095/biolreprod59.6.1445
  5. Eppig JJ, Schroeder AC (1989): Capacity of mouse oocytes from preantral follicles to undergo embryogenesis and development to live young after growth, maturation and fertilization in vitro. Biol Reprod 41: 268-276. https://doi.org/10.1095/biolreprod41.2.268
  6. Eppig JJ, Wigglesworth K (1995): Factors affecting the developmental competence of mouse oocytes grown in vitro: oxygen concentration. Mol Reprod Dev 42:447-456. https://doi.org/10.1002/mrd.1080420412
  7. Freeman BA, Crapo JD (1983): Biology of disease: free radicals and tissue injury. Lab Invest 47:412-426.
  8. Gardner DK (2008): Dissection of culture media for embryos: the most important and less important components and characteristics. Reprod Fertil Dev 20: 9-18.
  9. Jeong YW, Park SW, Hossein MS, Kim S, Kim JH, Lee SH, Kang SK, Lee BC, Hwang WS (2006): Antiapoptotic and embryotrophic effects of alpha- tocopherol and L-ascorbic acid on porcine embryos derived from in vitro fertilization and somatic cell nuclear transfer. Theriogenology 66:2104-2112. https://doi.org/10.1016/j.theriogenology.2006.06.007
  10. Kim DH, Ko DS, Lee HC, Lee HJ, Park WI, Kim SS, Park JK, Yang BC, Park SB, Chang WK, Lee HT (2004): Comparison of maturation, fertilization, development, and gene expression of mouse oocytes grown in vitro and in vivo. J Assist Reprod Genet 21: 233-240. https://doi.org/10.1023/B:JARG.0000042008.83699.cc
  11. Kim DH, No JG, Park JJ, Park JK, Yoo JG (2012): Successful in vitro development of preantral follilces isolated from vitrified mouse whole ovaries. Reprod Dev Biol 36:255-260. https://doi.org/10.12749/RDB.2012.36.4.255
  12. Kim DH, Chi, HJ, Kang HG, Han SW, Lee HT, Chung KS, Lee HJ (1999): Effects of gonadotrophins on in vitro growth and maturation of mouse preantral follicles. Kor J Ani Reprod 23:53-61.
  13. Legge M, Sellens MH (1991): Free radical scavengers ameliorate the 2-cell block in mouse embryo culture. Hum Reprod 6:867-871. https://doi.org/10.1093/oxfordjournals.humrep.a137442
  14. Li J, Foote RH (1993): Development of rabbit zygotes cultured in protein-free medium with catalase, taurine, or superoxide dismutase. Biol Reprod 49:33-37. https://doi.org/10.1095/biolreprod49.1.33
  15. Meister A, Anderson ME (1983): Glutathione. Annu Rev Biochem 52:711-760. https://doi.org/10.1146/annurev.bi.52.070183.003431
  16. Miller JG, Schultz GA (1987): Amino acid content of preimplantation rabbit embryos and fluids of the reproductive tract. Biol Reprod 36:125-129. https://doi.org/10.1095/biolreprod36.1.125
  17. Nasr-Esfahani MM, Johnson MH (1991): The origin of reactive oxygen species in mouse embryos cultured in vitro. Development 113:551-560.
  18. Nasr-Esfahani MH, Winston NJ, Johnson MH (1992): Effects of glucose, glutamine, ethylenediaminetetraacetic acid and oxygen tension on the concentration of reactive oxygen species and on development of the mouse preimplantation embryo in vitro. J Reprod Fertil 96:219-231. https://doi.org/10.1530/jrf.0.0960219
  19. Nayudu PL, Osborn SM (1992): Factors influencing the rate of preantral and antral growth of mouse ovarian follicles in vitro. J Reprod Fertil 95:349-362. https://doi.org/10.1530/jrf.0.0950349
  20. Orsi NM, Leese HJ (2001): Protection against reactive oxygen species during mouse preimplantation embryo development: role of EDTA, oxygen tension, catalase, superoxide dismutase and pyruvate. Mol Reprod Dev 59:44-53. https://doi.org/10.1002/mrd.1006
  21. Park YH, Gong SP, Kim HY, Kim GA, Choi JH, Ahn JY, Lim JM (2013): Development of a serum-free defined system employing growth factors for preantral follicle culture. Mol Reprod Dev 80:725-733.
  22. Park KE, Ku SY, Jung KC, Liu HC, Kim YY, Kim YJ, Kim SH, Choi YM, Kim JG, Moon SY (2013): Effects of urinary and recombinant gonadotropins on in vitro maturation outcomes of mouse preantral follicles. Reprod Sci 20:909-916. https://doi.org/10.1177/1933719112468948
  23. Schultz GA, Kaye PL, McKay DJ, Johnson MH (1981): Endogenous amino acid pool sizes in mouse eggs and preimplantation embryos. J Reprod Fertil 61:387-393. https://doi.org/10.1530/jrf.0.0610387
  24. Talebi A, Zavareh S, Kashani MH, Lashgarbluki T, Karimi I (2012): The effect of alpha lipoic acid on the developmental competence of mouse isolated preantral follicles. J Assist Reprod Genet 29:175-183. https://doi.org/10.1007/s10815-011-9706-6
  25. Tatemoto H, Sakurai N, Muto N (2000): Protection of porcine oocytes against apoptotic cell death caused by oxidative stress during in vitro maturation: role of cumulus cells. Biol Reprod 63:805-810. https://doi.org/10.1095/biolreprod63.3.805
  26. Thurston JH, Hauhart RE, Naccarato EF (1981): Taurine: possible role in osmotic regulation of mammalian heart. Science 214:1373-1374. https://doi.org/10.1126/science.7313699
  27. Wu GQ, Jia BY, Li JJ, Fu XW, Zhou GB, Hou YP, Zhu SE (2011): L-Carnitine enhances oocyte maturation and development of parthenogenetic embryos in pigs. Theriogenology 76:785-793. https://doi.org/10.1016/j.theriogenology.2011.04.011
  28. Yoshida M, Ishigaki K, Nagai T, Chikyu M, Pursel VG (1993): Glutathione concentration during maturation and after fertilization in pig oocytes: relevance to the ability of oocytes to form male pronucleus. Biol Reprod 49:89-94. https://doi.org/10.1095/biolreprod49.1.89
  29. Zhang ZP, Liang GJ, Zhang XF, Zhang GL, Chao HH, Li L, Sun XF, Min LJ, Pan QJ, Shi QH, Sun QY, De Felici M, Shen W (2012): Growth of mouse oocytes to maturity from premeiotic germ cells in vitro. PLoS One 7(7):e41771. https://doi.org/10.1371/journal.pone.0041771