International Journal of Oral Biology

  • 제38권4호
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  • Pages.141-147
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  • 2013
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  • 1226-7155(pISSN)
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  • 2287-6618(eISSN)
대한구강생물학회 (The Korean Academy of Oral Biology)
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Antimicrobial Activity of Berberine against Oral Bacteria Related to Endodontic Infections

  • Lee, Dongkyun (Department of Conservative Dentistry, School of Dentistry, Chosun University) ;
  • Kim, Min Jung (Korean Collection for Oral Microbiology, Department of Oral Biochemistry, and Oral Biology Research Institute, School of Dentistry, Chosun University) ;
  • Park, Soon-Nang (Korean Collection for Oral Microbiology, Department of Oral Biochemistry, and Oral Biology Research Institute, School of Dentistry, Chosun University) ;
  • Lim, Yun Kyong (Korean Collection for Oral Microbiology, Department of Oral Biochemistry, and Oral Biology Research Institute, School of Dentistry, Chosun University) ;
  • Min, Jeong-Beom (Department of Conservative Dentistry, School of Dentistry, Chosun University) ;
  • Hwang, Ho-Keel (Department of Conservative Dentistry, School of Dentistry, Chosun University) ;
  • Kook, Joong-Ki (Korean Collection for Oral Microbiology, Department of Oral Biochemistry, and Oral Biology Research Institute, School of Dentistry, Chosun University)
  • 투고 : 2013.09.17
  • 심사 : 2013.12.17
  • 발행 : 2013.12.31
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초록

It has been established that berberine has strong antimicrobial effects. Little is known however regarding the antimicrobial activity of berberine against endodontic pathogenic bacteria or its cytotoxicity in human oral tissue cells. The antibacterial properties of berberine were tested against 5 strains of Enterococcus faecalis and type strains of Aggregatibacter actinomycetemcomitans, Prevotella nigrescens, Prevotella intermedia, and Tannerella forsythia, which are involved in endodontic infections. Antimicrobial activity was evaluated through minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) measurements. The viability of normal human gingival fibroblast (NHGF) cells after exposure to berberine was measured using a methyl thiazolyl tetrazolium (MTT) assay. The data showed that berberine has antimicrobial effects against A. actinomycetemcomitans with an MIC and MBC of $12.5{\mu}g/ml$ and $25{\mu}g/ml$, respectively. In the cytotoxicity studies, cell viability was maintained at 66.1% following exposure to $31.3{\mu}g/ml$ berberine. Overall, these findings suggest that berberine has antimicrobial activity against the tested bacteria. Nevertheless, lower concentrations in combination with other reagents will need to be tested before these in vitro results can be translated to clinical use.

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참고문헌

  1. Trope M. The vital tooth: its importance in the study andpractice of endodontics. Endod Topics. 2003;5:1 https://doi.org/10.1111/j.1601-1546.2003.00031.x
  2. Schilder H. Cleaning and shaping the root canal. Dent Clin North Am. 1974;18:269-296.
  3. Dalton BC, Orstavik D, Phillips C, Pettiette M, Trope M. Bacterial reduction with nickel-titanium rotary instrumentation. J Endod. 1998;24:763-767. https://doi.org/10.1016/S0099-2399(98)80170-2
  4. Tik Tan B, Messer HH. The quality of apical canal preparation using hand and rotary instruments with specific criteria for enlargement based on initial apical file size. J Endod. 2002;28:658-664. https://doi.org/10.1097/00004770-200209000-00008
  5. Peters OA, Laib A, Göhring T, Barbakow F. Changes in root canal geometry after preparation assessed by high-resolution computed tomography. J Endod. 2001;27:1-6. https://doi.org/10.1097/00004770-200101000-00001
  6. Sundqvist G, Figdor D. Life as an endodontic pathogen: Ecological differences between the untreated and root-filled root canals. Endod Topics. 2003;6:3-28. https://doi.org/10.1111/j.1601-1546.2003.00054.x
  7. Mohammadi Z. Sodium hypochlorite in endodontics: an update review. Int Dent J. 2008;58:329-341. https://doi.org/10.1111/j.1875-595X.2008.tb00354.x
  8. Gomes BP, Ferraz CC, Vianna ME, Berber VB, Teixeira FB, Souza-Filho FJ. In vitro antimicrobial activity of several concentrations of sodium hypochlorite and chlorhexidine gluconate in the elimination of Enterococcus faecalis. Int Endod J. 2001;34:424-428. https://doi.org/10.1046/j.1365-2591.2001.00410.x
  9. Vianna ME, Gomes BPFA, Berber VB, Zaia AA, Ferraz CCR, de Souza-Filho FJ. In vitro evaluation of the antimicrobial activity of chlorhexidine and sodium hypochlorite. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2004;97:79-84. https://doi.org/10.1016/S1079-2104(03)00360-3
  10. Mohammadi Z, Abbott PV. The properties and applications of chlorhexidine in endodontics. Int Endod J. 2009;42:288-302. https://doi.org/10.1111/j.1365-2591.2008.01540.x
  11. White RR, Hays GL, Janer LR. Residual antimicrobial activity after canal irrigation with chlorhexidine. J Endod. 1997;23:229-231. https://doi.org/10.1016/S0099-2399(97)80052-0
  12. Khademi AA, Mohammadi Z, Havaee A. Evaluation of the antibacterial substantivity of several intra-canal agents. Aust Endod J. 2006;32:112-115. https://doi.org/10.1111/j.1747-4477.2006.00033.x
  13. Kanisavaran ZM. Chlorhexidine gluconate in endodontics: an update review. Int Dent J. 2008;58:247-257. https://doi.org/10.1111/j.1875-595X.2008.tb00196.x
  14. Arias-Moliz MT, Ferrer-Luque CM, Espigares-Rodriguez E, Liebana-Urena J, Espigares-Garcia M. Bactericidal activity of phosphoric acid, citric acid, and EDTA solutions against Enterococcus faecalis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2008;106:e84-89.
  15. Ates M, Akdeniz BG, Sen BH. The effect of calcium chelating or binding agents on Candida albicans. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2005;100:626-630. https://doi.org/10.1016/j.tripleo.2005.03.004
  16. Zehnder M. Root canal irrigants. J Endod. 2006;32:389-398. https://doi.org/10.1016/j.joen.2005.09.014
  17. Raad II, Fang X, Keutgen XM, Jiang Y, Sherertz R, Hachem R. The role of chelators in preventing biofilm formation and catheter-related bloodstream infections. Curr Opin Infect Dis. 2008;21:385-392. https://doi.org/10.1097/QCO.0b013e32830634d8
  18. Torabinejad M, Shabahang S, Aprecio RM, Kettering JD. The antimicrobial effect of MTAD: an in vitro investigation. J Endod. 2003;29:400-403. https://doi.org/10.1097/00004770-200306000-00005
  19. Ehrmann EH, Messer HH, Adams GG. The relationship of intracanal medicaments to postoperative pain in endodontics. Int Endod J. 2003;36:868-875. https://doi.org/10.1111/j.1365-2591.2003.00735.x
  20. Rossi-Fedele G, Guastalli AR, Dogramaci EJ, Steier L, De Figueiredo JAP. Influence of pH changes on chlorine-containing endodontic irrigating solutions. Int Endod J. 2011;44:792-799. https://doi.org/10.1111/j.1365-2591.2011.01911.x
  21. Poggio C, Arciola CR, Dagna A, Florindi F, Chiesa M, Saino E, Imbriani M, Visai L. Photoactivated disinfection (PAD) in endodontics: an in vitro microbiological evaluation. Int J Artif Organs 2011;34:889-897. https://doi.org/10.5301/ijao.5000056
  22. Birdsall TC, Kelly GS. Berberine: Therapeutic potential of an alkaloid found in several medicinal plants. Alt Med Rev. 1997;2:94-103.
  23. Sack RB, Froehlich JL. Berberine inhibits intestinal secretory response of Vibrio cholerae and Escherichia coli enterotoxins. Infect Immun. 1982;35:471-475.
  24. Cecil CE, Davis JM, Cech NB, Laster SM. Inhibition of H1N1 influenza A virus growth and induction of inflammatory mediators by the isoquinoline alkaloid berberine and extracts of goldenseal (Hydrastis canadensis). Int Immunopharmacol. 2011;11:1706-1714. https://doi.org/10.1016/j.intimp.2011.06.002
  25. Kosalec I, Gregurek B, Kremer D, Zovko M, Sankovic K, Karlovic K. Croatian barberry (Berberis croatica Horvat): a new source of berberine-analysis and antimicrobial activity. World J Microbiol Biotechnol. 2009;25:145-150. https://doi.org/10.1007/s11274-008-9860-x
  26. Ghosh, AK, Bhattacharyya FK, Ghosh DK. Leishmania donovani: Amastigote inhibition and mode of actior of berberine. Exp Parasitol. 1985;60:404-413. https://doi.org/10.1016/0014-4894(85)90047-5
  27. Sun Y, Xun K, Wang Y, Chen X. A systematic review of the anticancer properties of berberine, a natural product from Chinese herbs. Anticancer Drugs. 2009;20:757-769. https://doi.org/10.1097/CAD.0b013e328330d95b
  28. Clinical and Laboratory Standards Institute. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; Approved Standard-Sixth Edition. CLSI document M7-A6. Wayne Pennsylvania USA. 2005.
  29. Stermitz FR, Lorenz P, Tawara JN, Zenewicz LA, Lewis K. Synergy in a medicinal plant: antimicrobial action of berberine potentiated by 5'-methoxyhydnocarpin, a multidrug pump inhibitor. Proc Natl Acad Sci. 2000;97:1433-1437. https://doi.org/10.1073/pnas.030540597
  30. Bhandari DK, Nath G, Ray AB, Tewari PV. Antimicrobial activity of crude extracts from Berberis asiatica stem bark. Pharm Biol. 2000;38:254-257. https://doi.org/10.1076/1388-0209(200009)3841-AFT254
  31. Iwasa K, Lee DU, Kang SI, Wiegrebe W. Antimicrobial activity of 8-alkyl- and 8-phenyl-substituted berberines and their 12-bromo derivatives. J Nat Prod. 1998;61:1150-1153. https://doi.org/10.1021/np980044+
  32. Ozok AR, Persoon IF, Huse SM, Keijser BJF, Wesselink PR, Crielaard W, Zaura E. Ecology of the microbiome of the infected root canal system: a comparison between apical and coronal root segments. Int Endod J. 2012;45:530-541. https://doi.org/10.1111/j.1365-2591.2011.02006.x
  33. Chavez de Paz L. Gram-positive organisms in endodontic infections. Endod Topics. 2004;9:79-96. https://doi.org/10.1111/j.1601-1546.2004.00107.x
  34. Molander A, Reit C, Dahlen G, Kvist T. Microbiological status of root-filled teeth with apical periodontitis. Int Endod J.1998;31:1-7. https://doi.org/10.1046/j.1365-2591.1998.t01-1-00111.x
  35. Recas IN, Siqueira JF, Santos KRN. Association of Enterococcus faecalis with different forms of periradicular diseases. J Endod. 2004;30:315-320. https://doi.org/10.1097/00004770-200405000-00004
  36. Stuart CH, Schwartz SA, Beeson TH, Owatz CB. Enterococcus faecalis: its role in root canal treatment failure and current concept in retreatment. J Endod. 2006;32:93-98.
  37. Dornelles-Morgental R, Guerreiro-Tanomaru JM, de Faria-Junior NB, Hungaro-Duarte MA, Kuga MC, Tanomaru Filho M. Antibacterial efficacy of endodontic irrigating solutions and their combinations in root canals contaminated with Enterococcus faecalis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2011;112:396-400. https://doi.org/10.1016/j.tripleo.2011.02.004
  38. Endo MS, Martinho FC, Zaia AA, Ferraz CCR, Almeida JFA, Gomes BPFA. Quantification of cultivable bacteria and endotoxin in post-treatment apical periodontitis before and after chemo-mechanical preparation. Eur J Clin Microbiol Infect Dis. 2012;31:2575-2583 https://doi.org/10.1007/s10096-012-1598-6
  39. Gomes BPFA, Jacinto RC, Pinheiro ET, Sousa ELR, Zaia AA, Ferraz CCR, Souza-Filho FJ. Porphyromonas gingivalis, Porphyromonas endodontalis, Prevotella intermedia and Prevotella nigrescens in endodontic lesions detected by culture and by PCR. Oral Microbiol Immunol. 2005;20:211-215. https://doi.org/10.1111/j.1399-302X.2005.00214.x
  40. Cao H, Qi Z, Jiang H, Zhao J, Liu Z, Tang Z. Detection of Porphyromonas endodontalis, Porphyromonas gingivalis and Prevotella intermedia in primary endodontic infections in a Chinese population. Int Endod J. 2012:45:773-781 https://doi.org/10.1111/j.1365-2591.2012.02035.x
  41. Tronstad L, Sunde PT. The evolving new understanding of endodontic infections. Endod Topics. 2003;6:57-77. https://doi.org/10.1111/j.1601-1546.2003.00039.x
  42. Yu HH, Kim KJ, Cha JD, Kim HK, Lee YE, Choi NY. Antimicrobial activity of berberine alone and in combination with ampicillin or oxacillin against methicillin-resistant Staphylococcus aureus. J Med food. 2005;8:454-461. https://doi.org/10.1089/jmf.2005.8.454
  43. Moussa NMA, Xie Q, BeGole E, Johnson B, Wenckus C, Wu C. The antimicrobial efficacy of berberine versus sodium hypochlorite and chlorhexidine against E. faecalis. J Endod. 2010;36:572.
  44. Hu JP, Takahashi N, Yamada T. Coptidis rhizoma inhibits growth and proteases of oral bacteria. Oral Dis. 2000;6:297-302.
  45. Ghosh AK, Bhattacharyya FK, Ghosh DK. Leishmania donovani: Amastigote inhibition and mode of actior of berberine. Exp Parasitol. 1985;60:404-413. https://doi.org/10.1016/0014-4894(85)90047-5
  46. Sun D, Courtney HS, Beachey EH. Berberine sulfate blocks adherence of Streptococcus pyogenes to epithelial cells, fibronectin, and hexadecane. Antimicrob Agents Chemother. 1988;32:1370-1374. https://doi.org/10.1128/AAC.32.9.1370
  47. Wu L-T, Tsou M-F, Ho C-C, Chuang J-Y, Kuo H-M, Chung J-G. Berberine inhibits arylamine N-acetyltransferase activity and gene expression in Salmonella typhi. Curr Microbiol. 2005;51:255-261. https://doi.org/10.1007/s00284-005-4569-7
  48. Haapasalo M, Shen Y, Qian W, Gao Y. Irrigation in endodontics. Dent Clin North Am. 2010;54:291-312. https://doi.org/10.1016/j.cden.2009.12.001
  49. Zhang W, Torabinejad M, Li Y. Evaluation of cytotoxicity of MTAD using the MTT-tetrazolium method. J Endod. 2003;29:654-657. https://doi.org/10.1097/00004770-200310000-00010
  50. Hulsmann M, Hahn W. Complications during root canal irrigation-literature review and case reports. Int Endod J. 2000;33:186-193. https://doi.org/10.1046/j.1365-2591.2000.00303.x