Korean Journal of Clinical Laboratory Science (대한임상검사과학회지)
- Volume 45 Issue 1
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- Pages.21-25
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- 2013
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- 1738-3544(pISSN)
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- 2288-1662(eISSN)
Errors of Antibiotic Susceptibility Testing from Automated and Manual Systems in Clinical Isolates of Acinetobacter baumannii
- Sung, Ji Youn (Department of Biomedical Laboratory Science, Far East University) ;
- Oh, Ji-Eun (Department of Biomedical Laboratory Science, Far East University) ;
- Kim, Eun Sun (Department of Biomedical Laboratory Science, Far East University)
- Received : 2013.01.29
- Accepted : 2013.03.13
- Published : 2013.03.30
Abstract
Acinetobacter baumannii is an aerobic, gram-negative and glucose-non-fermenting bacterium, which has emerged as a serious opportunistic pathogen. Many clinical microbiology laboratories use the Vitek 2 system for the routine antimicrobial susceptibility testing process, including testing on A. baumannii isolates. However, in case of amikacin, it is now recommended to perform additional antimicrobial susceptibility testing for A. baumannii strains due to the relatively lower minimum inhibitory concentration (MIC) in the Vitek 2 system compared to conventional reference methods. In our study, we assessed MIC for amikacin susceptibility testing of A. baumannii isolates in the Vitek 2 system, the agar dilution, Etest, and disk diffusion method. We collected 40 gentamicin-resistant, A. baumannii strains (amikacin MIC by Vitek 2: