Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
권호 표지
DOI QR코드

DOI QR Code

The effect of extract from leaves and stalks of Angelica gigas on the innate immunity

  • Kang, Shin-Seok (Chungbuk Veterinary Service Laboratory) ;
  • Byeon, Hyeon-Seop (Chungbuk Veterinary Service Laboratory) ;
  • Kang, Shin-Kwon (Division of Food Science, International University of Korea) ;
  • Ko, Duk-Hwan (Department of Orthopaedic Surgery, Medical School, Konkuk University) ;
  • Lim, Dae-Jun (Department of Orthopaedic Surgery, Medical School, Konkuk University) ;
  • Lee, Jung-Hwa (Department of Orthopaedic Surgery, Medical School, Konkuk University) ;
  • Kang, Sung-Ho (Department of Orthopaedic Surgery, Medical School, Konkuk University)
  • Received : 2013.11.04
  • Accepted : 2013.12.26
  • Published : 2013.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

The dried root of Angelica gigas (A. gigas) has been traditionally used as an oriental medicine, which is known to improve blood circulation and blood stasis. In the present study, leaves and stalks of A. gigas were used to investigate their effects on the innate immunity. The extracts were prepared from leaves and stalks of A. gigas and were fed to mice. The numbers of blood cells, total WBCs, neutrophils, lymphocytes, eosinophils and basophils were increased by 50% in mice fed with leaves extract of A. gigas compared to control mice. However, the numbers of blood cells were decreased when treated with stalks extract of A. gigas. The level of cholesterol and triglyceride in serum was markedly reduced in both mice group fed with leaves extract and stalks extract of A. gigas compared to control group (P<0.01). There was no significant change in the level of albumin, total protein, phosphate and calcium in serum. Activity of cationic peptide was found to be diffused in the testicles of mice fed with leaves extract of A. gigas compared to control group, which might be due to increased lysozyme in testicle. The lysoplate assay and immunohistochemistry assay suggest that the extract of leaves and stalks of A. gigas are immunogenic, but the effects might be related with acquired immune response rather than innate immunity.

Keywords

References

  1. Ahn MJ, Lee MK, Kim YC, Sung SH. 2008. The simultaneous determination of coumarins in Angelica gigas root by high performance liquid chromatography-diode array detector coupled with electrospray ionization/mass spectrometry. J Pharm Biomed Anal 46: 258-266. https://doi.org/10.1016/j.jpba.2007.09.020
  2. Canfield RE. 1963. The amino acid sequence of egg white Lysozyme. J Biol Chem 238: 2698-2707.
  3. Charles AJ Jr, Rulsan M. 2002. Innate immune recognition. Annu Rev Immunol 20: 197-216. https://doi.org/10.1146/annurev.immunol.20.083001.084359
  4. Chi HJ, Kim HS. 1970. Studies on the components of umbelliferate plants in Korea: pharmacological study of decursin, decursinol and nodakenin. Korean J Pharmacogn 1:25-32.
  5. Choi SS, Han KJ, Lee HK, Han EJ, Suh HW. 2003. Antinociceptive profiles of crude extract from roots of Angelica gigas Nakai in various pain models. Biol Pharm Bull 26:1283-1288. https://doi.org/10.1248/bpb.26.1283
  6. Cole AM, Dewan P, Ganz T. 1999. Innate antimicrobial activity of nasal secretions. Infect Immun 67: 3267-3275.
  7. Fleming A. 1922. On a remarkable bacteriolytic element found in tissue and secretions. Proc R Soc Lond B 93: 306-317. https://doi.org/10.1098/rspb.1922.0023
  8. Ganz T. 2004. Antimicrobial polypeptides. J Leukoc Biol 75:34-38.
  9. Hankiewicz J, Swierczek E. 1974. Lysozyme in human fluid. Clin Chim Acta 57: 205-209. https://doi.org/10.1016/0009-8981(74)90398-2
  10. Hoffmann JA, Kafatos FC, Janeway CA, Ezekowitz RA. 1999. Phylogenetic perspectives in innate immunity. Science 284: 1313-1318. https://doi.org/10.1126/science.284.5418.1313
  11. Konstan MW, Cheng PW, Boat TF. 1982. A comparative study of lysozyme and its secretion by tracheal epithelium. Exp Lung Res 3: 175-181. https://doi.org/10.3109/01902148209063291
  12. Kumazawa Y, Mizunoe K, Otsuka Y. 1982. Immunostimulating polysaccharide seperated from hot water extract of Angelica acutiloba Kitakawa (Yamato tohki). Immunology 47: 75-83.
  13. Kumazawa Y, Nakatsuru Y, Fujisawa H, Nishimura C, Mizunoe K, Otsuka Y, Nomoto K. 1985. Lymphocyte activation by a polysaccharide fraction seperated from hot water extracts of Angelica acutiloba Kitakawa. J Pharmacobiodyn 8: 417-424. https://doi.org/10.1248/bpb1978.8.417
  14. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. 1951. Protein measurement with folin phenol reagent. J Biol Chem 193: 265-275.
  15. Osserman EF, Lawlor DP. 1966. Serum and urinary lysozyme (muramidase) in monocytic and monomyelocytic leukemia. J Exp Med 124: 921-952. https://doi.org/10.1084/jem.124.5.921
  16. Phillips DC. 1966. The Three-dimensional structure of an enzyme molecule. Sci Am 215: 78-90.
  17. Prager EM, Jolles P. 1996. Animal lysozymes c and g: an overview. EXS 75: 9-31.
  18. Shin S, Jeon JH, Park D, Jang JY, Joo SS, Hwang BY, Choe SY, Kim YB. 2009. Anti-inflammatory effects of an ethanol extract of Angelica gigas in a Carrageenan-air pouch inflammation model. Exp Anim 58: 431-436. https://doi.org/10.1538/expanim.58.431
  19. Vidal ML, Gautron J, Nys Y. 2005. Development of an ELISA for quantifying lysozyme in hen egg white. J Agric Food Chem 53: 2379-2385. https://doi.org/10.1021/jf048692o
  20. Yoon T, Cheon MS, Lee DY, Moon BC, Lee HW, Choo BK, Kim HK. 2007. Effects of root extract from Angelica gigas and Angelica acutiloba on inflammatory mediators in mouse macrophages. J Appl Biol Chem 50: 264-269.

Cited by

  1. 당귀의 광합성 특성과 수경재배 방식에 따른 생장 분석 vol.41, pp.4, 2013, https://doi.org/10.7744/cnujas.2014.41.4.321