Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Genetic Diversity of Curcuma Genus Collected Germplasm using Analysis of AFLP

AFLP 분석을 통한 Curcuma속 수집종간 유전적 다양성 분석

  • Moon, Byeong Cheol (Herbal Medicine Resources Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Kim, Wook Jin (Herbal Medicine Resources Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Ji, Yunui (Herbal Medicine Resources Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Lee, Young Mi (Herbal Medicine Resources Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Kim, Ho Kyoung (Herbal Medicine Resources Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine)
  • 문병철 (한국한의학연구원 한약연구본부 한약자원그룹) ;
  • 김욱진 (한국한의학연구원 한약연구본부 한약자원그룹) ;
  • 지윤의 (한국한의학연구원 한약연구본부 한약자원그룹) ;
  • 이영미 (한국한의학연구원 한약연구본부 한약자원그룹) ;
  • 김호경 (한국한의학연구원 한약연구본부 한약자원그룹)
  • Received : 2013.10.15
  • Accepted : 2013.11.22
  • Published : 2013.12.30
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Abstract

Collected germplasms of five representative species belonging to Curcuma genus (C. longa, C. aromatica, C. zedoaria, C. phaeocaulis and C. kwangsiensis) were 52 samples from different farmhouse in Korea and China. To elucidate the genetic diversity among the species, 52 samples were analyzed by genomic fingerprinting method using amplified fragment length polymorphism (AFLP). AFLP results of 6 primer combinations were revealed 643 total DNA fragments and 349 polymorphic bands with the 54.3% ratio of polymorphism. In the analysis of coefficient similarity using unweight pair group method with arithmetic averages (UPGMA), 52 Curcuma germplasm lines were ranged from 0.60 to 0.99 and clustered distinct five groups according to the species and collected geographical levels. However, the result of principal coordinate analysis (PCA) by multi-variate analysis was shown significantly greater differences among species than geographical origins based on AFLP profiling data of these samples.

Keywords

References

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