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Single Intramuscular-dose Toxicity of Anti-inflammatory Pharmacopuncture in Rats

  • Jung, Da-Jung (Department of Acupuncture & Moxibustion Medicine, Woosuk University Hospital of Korean Medicine) ;
  • Kim, Sung-Chul (Department of Acupuncture & Moxibustion Medicine, Wonkwang University Hospital of Korean Medicine) ;
  • Lee, Hyung-Geol (Department of Acupuncture & Moxibustion Medicine, Woosuk University Hospital of Korean Medicine) ;
  • Choi, Yoo-Min (Department of Acupuncture & Moxibustion Medicine, Woosuk University Hospital of Korean Medicine) ;
  • Sin, Min-Seop (Department of Acupuncture & Moxibustion Medicine, Woosuk University Hospital of Korean Medicine) ;
  • Choi, Seok-Woo (Department of Acupuncture & Moxibustion Medicine, Woosuk University Hospital of Korean Medicine) ;
  • Hong, Seung-Won (MyungGa Oriental Medical Clinic) ;
  • Song, Beom-Yong (Department of Acupuncture & Moxibustion Medicine, Woosuk University Hospital of Korean Medicine) ;
  • Kim, Jong-Uk (Department of Acupuncture & Moxibustion Medicine, Woosuk University Hospital of Korean Medicine) ;
  • Yook, Tae-Han (Department of Acupuncture & Moxibustion Medicine, Woosuk University Hospital of Korean Medicine)
  • 투고 : 2013.08.03
  • 심사 : 2013.11.07
  • 발행 : 2013.12.31

초록

Objectives: This study was performed to analyze the toxicity of the test substance, anti-inflammatory pharmacopuncture (AIP), when used as a single intramuscular-dose in 6-week-old, male and female Sprague-Dawley rats and to find the lethal dose. Methods: The experiment was conducted at Biotoxtech according to Good Laboratory Practices. Twenty (20) female and 20 male Spague-Dawley rats were divided into 4 groups of five 5 female and 5 male animals per group. The rats in the three experimental groups received single intramuscular injections with 0.1-$m{\ell}$, 0.5-$m{\ell}$ and 1.0-$m{\ell}$/animal doses of AIP, Groups 2, 3, and 4, respectively, and the control group, Group 1, received a single intramuscular injection with a 1.0-$m{\ell}$ dose of normal saline. Clinical signs were observed and body weight measurements were carried out for 14 days following the injections. At the end of the observation period, hematology, clinical chemistry, histopathological tests and necropsy were performed on the injected parts. Results: No deaths occurred in any of the groups. Also, histopathological tests showed that AIP had no effect on the injected parts in terms of clinical signs, body weight, hematology, clinical chemistry, and necropsy. Conclusions: As a result of single intramuscular-dose tests of the test substance AIP in 4 groups of rats, the lethal dose for both males and females exceeded $1.0m{\ell}$/animal. Therefore, AIP is a relatively safe pharmacopuncture that can be used for treatment, but further studies should be performed.

키워드

참고문헌

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피인용 문헌

  1. Toxicity of Single-dose Intramuscular Injection of Samjeong Pharmacopuncture in Sprague-Dawley Rats vol.18, pp.2, 2015, https://doi.org/10.3831/KPI.2015.18.016
  2. Herbal Acupuncture for the Treatment of Obesity vol.9, pp.2, 2016, https://doi.org/10.1016/j.jams.2015.09.002