Journal of agriculture & life science (농업생명과학연구)

Institute of Agriculture & Life Science, Gyeongsang National University (경상대학교 농업생명과학연구원)
권호 표지

Effect of Lignans Isolated from Schisandra chinensis Baillon on Seed Germination and Seedling Growth in Radish

오미자 (Schisandra chinensis Baillon)로부터 순수분리한 리그난이 무의 종자발아 및 유묘생육에 미치는 영향

  • Park, Da-Jung (Dept. of Horticultural Bioscience, Natural Resources and Life Science, Pusan National Univ.) ;
  • Kim, Youn-Han (Dept. of Horticultural Bioscience, Natural Resources and Life Science, Pusan National Univ.) ;
  • Park, Se-Jin (Dept. of Horticultural Bioscience, Natural Resources and Life Science, Pusan National Univ.) ;
  • Rajasekar, Seetharaman (Dept. of Horticultural Bioscience, Natural Resources and Life Science, Pusan National Univ.) ;
  • Park, Young-Hoon (Dept. of Horticultural Bioscience, Natural Resources and Life Science, Pusan National Univ.) ;
  • Kang, Jum-Soon (Dept. of Horticultural Bioscience, Natural Resources and Life Science, Pusan National Univ.) ;
  • Son, Beung-Gu (Dept. of Horticultural Bioscience, Natural Resources and Life Science, Pusan National Univ.) ;
  • Lee, Yong-Jae (Dept. of Horticultural Bioscience, Natural Resources and Life Science, Pusan National Univ.) ;
  • Kim, Sun-Tae (Dept. of Plant Bioscience, Natural Resources and Life Science, Pusan National Univ.) ;
  • Yoon, Moo-Kyoung (National Institute of Horticultural & Herbal Science, Pusan National Univ.) ;
  • Choi, Young-Whan (Dept. of Horticultural Bioscience, Natural Resources and Life Science, Pusan National Univ.)
  • 박다정 (부산대학교 원예생명과학과) ;
  • 김윤한 (부산대학교 원예생명과학과) ;
  • 박세진 (부산대학교 원예생명과학과) ;
  • ;
  • 박영훈 (부산대학교 원예생명과학과) ;
  • 강점순 (부산대학교 원예생명과학과) ;
  • 손병구 (부산대학교 원예생명과학과) ;
  • 이용재 (부산대학교 원예생명과학과) ;
  • 김선태 (부산대학교 식물생명과학과) ;
  • 윤무경 (부산대학교 원예특작과학원 채소과) ;
  • 최영환 (부산대학교 원예생명과학과)
  • Received : 2012.02.01
  • Accepted : 2012.02.24
  • Published : 2012.02.28
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Abstract

The aim of this study was to develop a rapid and cheap bioassay of four lignans (schisandrin, schisandrin C, the gomisin A and gomisin N) isolated from Schizandra chinensis Baill on seed germination and seedling growth of the radish. Its structure was determined by analysis of MS and NMR spectroscopic data. Radish seeds immersed for 1 hr in the solutions of $10^{-5}M$, $10^{-6}M$ and $10^{-7}M$ of schisandrin, schisandrin C, gomisin A, and gomisin N, seed germination was observed with in 60 hr after all of the treatments. Also, the seeds were germinated faster compared to untreated controls. At early germination stage, 48 hr after the treatment of the lignans, the suppression of germination was observed from all treatments; the suppression due to schisandrin and gomisin A was the highest at the concentration of $10^{-6}M$. The level of suppression increased as the concentration increased in the treatment of schisandrin C and gomisin N. Percent germination of seed after 184 hr was increased 90% at all treatments. For the effects of lignan treatment on seedling growth in radish, the growth of hypocotyl was promoted by gomisin A and gomisin N at all concentrations. Root elongation was significantly promoted by schisandrin and gomisin N at $10^{-5}M$ and $10^{-6}M$, respectively. Fresh and dry weights of the seedlings were high at a low concentration of $10^{-7}M$, but significantly reduced by schisandrin C at a high concentration of $10^{-5}M$. The results of the germination activity and seedling growth of the lignans from S. chinensis suggest their potential use as natural growth regulators.

본 연구의 목적은 오미자로부터 순수분리한 리그난이 무의 종자발아 및 유묘생장에 미치는 영향을 조사함으로서 생장조절제와 천연물의 생물검증 방법을 개발하기 위해서 수행하였다. 오미자로부터 순수 분리한 schisandrin, schisandrin C, gomisin A 및 gomisin N 등 4종의 리그난이 무 종자의 발아 및 유묘생육에 미치는 영향을 조사하였다. 무 종자를 $10^{-5}$, $10^{-6}$ 및 $10^{-7}M$의 schisandrin, schisandrin C, gomisin A 및 gomisin N 용액에 1시간 침지 후 발아율을 비교한 결과, 4종의 리그난류 처리는 대조구에 비해 발아속도가 빨랐다. 리그난류의 처리 48시간 후 대조구를 기준으로 하여 발아율의 촉진 또는 억제 효과를 비교한 결과, schisandrin과 gomisin A는 $10^{-6}M$의 농도에서, schisandrin C와 gomisin N은 농도가 높을수록 발아율이 억제되는 경향이었다. 리그난이 유묘생육에 미치는 영향을 대조구를 기준으로 하여 생육 촉진 또는 억제 효과를 조사한 결과, 하배축은 gomisin A와 gomisin N의 처리시 $10^{-5}M$의 고농도에서, gomisin N의 경우에는 $10^{-6}M$에서 생육이 촉진되었으며, 뿌리의 길이는 schisandrin $10^{-4}M$의 고농도에서 가장 길었다. 유식물체당 총 생체중 및 건물중은 $10^{-7}M$의 저농도 처리시에 촉진되었으며, schisandrin C는 $10^{-5}M$의 고농도 처리시에 생체중과 건물중이 감소하였다. 따라서 오미자로부터 순수분리한 리그난은 종자발아 또는 유묘의 생장을 조절할 수 있는 생장조절제로서 이용가능이 있을 것으로 기대된다.

Keywords

Acknowledgement

Supported by : 부산대학교

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