Journal of agriculture & life science (농업생명과학연구)

Institute of Agriculture & Life Science, Gyeongsang National University (경상대학교 농업생명과학연구원)
권호 표지

Determination of Polar Secondary Metabolomes in Arabidopsis thaliana using High Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry

  • Cho, Young-Ah (Dept. of Chemistry and Research Insti. of Life Sci., Gyeongsang National Univ.) ;
  • Park, Se-min (Dept. of Chemistry and Research Insti. of Life Sci., Gyeongsang National Univ.) ;
  • Bae, Dong-Won (Center for Research Facility, Gyeongsang National Univ.) ;
  • Seo, On-Nuri (Dept. of Chemistry and Research Insti. of Life Sci., Gyeongsang National Univ.) ;
  • Lee, Ji-Eun (Dept. of Chemistry and Research Insti. of Life Sci., Gyeongsang National Univ.) ;
  • Jeong, Sung-Woo (Dept. of Chemistry and Research Insti. of Life Sci., Gyeongsang National Univ.) ;
  • Kwon, Young-Sang (Division of Applied Life Science (BK21 program), Plant Melecular Biology & Biotechnology Research Center, Gyeongsang National Univ.) ;
  • Cha, Jae-Yul (Department of Applied Biology and Institute of Agricultural & Life Science, Gyeongsang National Univ.) ;
  • Bae, Han-Hong (School of Biotechnology, Yeungnam Univ.) ;
  • Shin, Sung-Chul (Dept. of Chemistry and Research Insti. of Life Sci., Gyeongsang National Univ.)
  • Received : 2012.09.05
  • Accepted : 2012.12.26
  • Published : 2012.12.31
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Abstract

As a preceding study for investigating the influence of sound wave stimulus on Arabidopsis thaliana metabolomics, the polar secondary metabolomes of the plant were determined using high performance liquid chromatography coupled with tandem mass spectrometry. A total of 10 polar secondary metabolomes were characterized and quantified. Among them, 4 metabolomes, p-coumaroylagmatine isomer (7 and 8), p-coumaroylagmatine isomer (9 and 10) were identified in the plant for the first time. The validation was conducted in terms of linearity, recovery, precision, limit of detection (LOD) and limit of quantification (LOQ). The validated method was applied to the simultaneous quantification of the 10 polar secondary metabolomes.

Keywords

Acknowledgement

Supported by : Rural Development Administration

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