DOI QR코드

DOI QR Code

참모자반 메탄올 추출 분획물의 항산화 및 숙취해소능과 α-glucosidase 활성저해효과

α-Glucosidase Inhibitory Effects for Solvent Fractions from Methanol Extracts of Sargassum fulvellum and Its Antioxidant and Alcohol-Metabolizing Activities

  • 강수희 (신라대학교 의생명과학대학 식품영양학과) ;
  • 조은경 (신라대학교 의생명과학대학 식품영양학과) ;
  • 최영주 (신라대학교 의생명과학대학 식품영양학과)
  • Kang, Su Hee (Department of Food and Nutrition, College of Medical Life Sciences, Silla University) ;
  • Cho, Eun Kyung (Department of Food and Nutrition, College of Medical Life Sciences, Silla University) ;
  • Choi, Young Ju (Department of Food and Nutrition, College of Medical Life Sciences, Silla University)
  • 투고 : 2012.09.20
  • 심사 : 2012.10.24
  • 발행 : 2012.10.30

초록

참모자반 분획물은 극성에 따라 핵산, 메탄올, 부탄올 및 수층 순으로 분획하여 4개의 분획층을 얻었으며, 각 분획층의 생리활성을 요약하면 다음과 같다. 항산화 활성은 DPPH radical 소거능과 SOD 활성을 측정한 결과 모두 농도 의존적으로 증가하였으며 특히 메탄올 분획 층에서 가장 높은 항산화 효과를 보였다. 아질산염 소거능 실험에서는 부탄올 층이 pH 1.2에서 57.8%로 4개의 분획물중 가장 높은 아질산염 소거능을 나타냈다. 숙취 해소능을 측정하기 위하여 ADH 활성과 ALDH 활성을 측정하였다. 대조구로 사용한 흡광도 값을 100으로 하였을 때 positive control로 사용한 hepos가 150~167%의 활성을 나타내었으며 4가지 분획층 모두 농도 의존적으로 활성이 증가하였다. 특히 ALDH에서는 메탄올 분획층이 10 mg/ml 농도에서 167.4%의 활성을 나타내어 hepos와 비슷한 효과를 보였다. 참모자반 분획물의 혈당강하 효과는 ${\alpha}$-glucosidase 활성억제 효과로 측정하였다. ${\alpha}$-glucosidase 활성억제 효과는 핵산 분획층에서 2 mg/ml의 농도에서 94.1%로 아주 높은 항당뇨 효과를 나타내었다. 그런데 부탄올층, 메탄올층 및 수층은 혈당강하효과가 거의 없는 것으로 나타났다. 피부주름예방을 측정하기 위하여 수행한 elastase 저해효과 실험에서 메탄올 분획층에서 농도 의존적으로 높은 주름개선효과가 나타났으며 2 mg/ml 농도에서 93.2%의 효과를 나타냈으며, positive control로 사용한 Vit. C (1 mg/ml) 보다 더 높은 활성을 나타냈다. 이러한 결과는 모자반 메탄올 추출물 중 특히 메탄올 분획층이 탁월한 주름개선 효과, 숙취 해소능과 항산화 효과가 있는 것으로 사료되며, 핵산층은 혈당강하 효과가 높은 것으로 나타났다. 따라서 모자반 유기용매 분획물은 천연 기능성소재로서의 사용 가능성이 매우 높을 것으로 사료된다.

We investigated the physiological activity and solvent-partitioned fractions of methanol extracts from the green seaweed Sargassum fulvellum. The methanol extract from S. fulvellum was sequentially fractionated with n-hexane (SFMH), methanol (SFMM), buthanol (SFMB), and water (SFMA). We investigated the antioxidant activities of solvent fractions from S. fulvellum by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity and an SOD activity assay. DPPH radical scavenging capacity of SFMM was 79.5% at 10 mg/ml. SOD activity of SFMM was 79.9% at 10 mg/ml. Nitrite scavenging activities of solvent fractions from S. fulvellum were investigated under different pH conditions and showed the most remarkable effect at pH 1.2. In particular, the activity of SFMB was higher than the other fractions. ADH activity and ALDH activity of SFMM were 177.0% and 167.4% at 10 mg/ml, respectively. ${\alpha}$-Glucosidase inhibitory activity of SFMH increased in a dose-dependent manner and was about 94.1% at 2 mg/ml. Elastase inhibitory activity was 93.2% at 2 mg/ml. These results revealed that S. fulvellum extracts have strong antioxidant and alcohol dehydrogenase activities and ${\alpha}$-glucosidase inhibitory activity, suggesting that S. fulvellum extracts have potential as a source of natural products for health and beauty.

키워드

참고문헌

  1. Ahn, S. M., Hong, Y. K., Kwon, G. S. and Shon, H. Y. 2011. Evaluation of antioxidant and nitrite scavenging activity of seaweed extracts. J. Life Sci. 21, 576-583. https://doi.org/10.5352/JLS.2011.21.4.576
  2. Bae, S. J. 2004. Anticarcinogenic effect of Sargassum fulvellum fractions on sever hum cancer cell lines in vito. J. Korean Soc. Food Sci. Nutr. 33, 480-486. https://doi.org/10.3746/jkfn.2004.33.3.480
  3. Blois, M. S. 1958. Antioxidant determination by the use of a stable free radical. Nature 26, 1198-1200.
  4. Bu, H.-J., Ham, M. H., Kim, J. M., Lee, S.-J., Hyun, J. W. and Lee, N. H. 2006. Elastase and hyaluronidase inhibition activities of phlorotannins isolated from Ecklonia cava. Korean J. Phamacogn. 37, 92-96.
  5. Cho, S.-H., Kang, S.-E., Cho, J.-Y., Kim, A.-R., Park, S.-M., Hong, Y.-K. and Ahn, D.-H. 2007. The antioxidant properties of brown seaweed (Sargassum siliquastrum) extracts. J. Med. Food. 10, 479-485. https://doi.org/10.1089/jmf.2006.099
  6. Cho, E. K., Yoo, S. K. and Choi, Y. J. 2011. Inhibitory effects of Maesaengi (Capsosiphon fulvescens) extracts on angiotensin converting enzyme and $\alpha$-glucosidase. J. Life Sci. 21, 811-818. https://doi.org/10.5352/JLS.2011.21.6.811
  7. Choi, J. T., Joo. H. K. and Lee. S. K. 1995. The effect of schizandrae fruits extract on alcohol fermentation and enzyme activities of Saccharomyces cervisiae. Agric. Chem. Biotechnol. 38, 278-282.
  8. Choi, S. Y., Kim, S. Y., Hur, J. M., Choi, H. G. and Sung, N. J. 2006. Antioxidant activity of solvent extracts from Sargassum thunbergii. J. Korean Soc. Food Sci. Nutr. 35, 139-144. https://doi.org/10.3746/jkfn.2006.35.2.139
  9. Gray, J. I and Dugan, Jr L. R. 1975. Inhibition of N-nitrosamine formation in model food systems. J. Food Sci. 40, 981-984. https://doi.org/10.1111/j.1365-2621.1975.tb02248.x
  10. Haung, H. L. and Wang, B. G. 2004. Antioxidant capacity and lipophilic content of seaweeds collected from Qingdao coastline. J. Agric. Food Chem. 52, 4993-4997. https://doi.org/10.1021/jf049575w
  11. Heo, S.-J., Park, E.-J., Lee, K.-W. and Jeon, Y.-J. 2005. Antioxidant activities of enzymatic extracts from brown seaweeds. Bioresources Technol. 96, 1613-1623. https://doi.org/10.1016/j.biortech.2004.07.013
  12. Heo, S. J. and Jeon, Y. J. 2005. Antioxidant effect and protecting effect against cell damage by enzymatic hydrolysates from marine algae. Food Industry Nutr. 10, 31-41.
  13. Hwang, P.-A., Chien, S.-Y., Chan, Y.-L., Lu, M. K., Wu, C.-H., Kong, Z.-L. and Wu, C.-J. 2001. Inhibition of lipopolysaccharide (LPS)-induced inflammatory responses by Sargassum hemiphyllum sulfated polysaccharide extract in RAW 264.7 macrophage cells. J. Agric. Food Chem. 59, 2062-2068.
  14. James, A. E. K., Timothy, D. W. and Gorden, L. 1996. Inhibition of human leukocyte and porcine pancreatic elastase by homologues of bovine pancreatic tyrosinase inhibitors. Biochem. 35, 9090-9096. https://doi.org/10.1021/bi953013b
  15. Kim, J. Y., Lee, J. A., Kim, K. N., Yoon, W. J., Lee W. J. and Park, S. Y. 2007. Antioxidative and antimicrobial activities of Sargassum muticum Extracts. J. Korean Soc. Food Sci. Nutr. 36, 663-669. https://doi.org/10.3746/jkfn.2007.36.6.663
  16. Kim, M. H., Chung, Y. T., Lee, J. H., Park, Y. S., Shin, M. K., Kim, H. S., Kim, D. H. and Lee, H. Y. 2000. Hepatic detoxification activity and reduction of serum alcohol concentration of Hovenia dulicis THUNB from Korea and China. Korean J. Medicinal Crop Sci. 8, 225-233.
  17. Kim, S. H., Choi, D. S., Athukorala, Y., Jeon, Y. J., Senevirathne, M. and Rha, C. K. 2007. Antioxidant activity of sulfated polysaccharides isolated from Sargassum fulvellum. J. Food Sci. Nutr. 12, 65-73. https://doi.org/10.3746/jfn.2007.12.2.065
  18. Kim, S. N. and Choi, H. Y., Lee, W., Park, G. M., Shin, W. S. and Kim, Y. K. 2008. Sargaquinolic acid and sargahydroquinolic acid from Sargassum yezoense stimulate adipocyte differentiation through PPARalpha/gamma activation in 3T3-L1 cells. FEBS Lett. 582, 3465-3472. https://doi.org/10.1016/j.febslet.2008.09.011
  19. Kong, C. S., Um, Y. R., Lee, J. I., Kim, Y. A., Lee, J. S. and Seo, Y. W. 2008. Inhibition effects of extracts and its solvent fractions isolated from Limanium tetragonum on growth of human cancer cells. Korean J. Biotechnol. Bioeng. 23, 177-182.
  20. Koo, J. G., Choi, Y. S. and Kwak, J. K. 2001. Blood-anticoagulant activity of fucoidans from sporophylls of Undaria pinnatifia, Laminaria religiosa, Hizikia fusiforme and Sargassum fulvellum in Korea. J. Korean Fish Soc. 34, 515-520.
  21. Kwak, S. C., Kim, S. A. and Lee, M. S. 2005. The correlation of antioxidative effects of 5 Korean common edible seaweeds and total polyphenol content. J. Korean Soc. Food Sci. Nutr. 34, 1143-1150. https://doi.org/10.3746/jkfn.2005.34.8.1143
  22. Kwak. Y. J., Lee. D. H., Kim. N. M. and Lee. J. S. 2005. Screening and extraction condition of anti-skin aging elastase inhibitor from medical plants. Korean J. Medicinal Crop Sci. 13, 213-216.
  23. Lee, B. H., Choi, B. W., Chun, J. H. and Yu, B. S. 1996. Extraction of water soluble antioxidants from seaweeds. J. Korean Ind. Eng. Chem. 7, 1069-1077.
  24. Lee, D. W., Ko, J. G., Ko, H. R., Park, S. J., Ryu, H. J., Kim, H. C., Cho, J. W., Ha, J. H. and Ko, S. B. 2011. Development of olle tea using Jeju fucoidan. The Korea academia-industrial cooperation society.
  25. Lee, E. H., Han, J., Ahn, H. R., Kim, M. C., Kim, C. Y., Pan, C. H., Um, B. H. and Jung, S. H. 2009. Inhibitory effects of the compounds isolated from Sargassum yezoense on -${\alpha}$-glucosidase and oxidative stress. Korean J. Pharmacogn. 40, 150-154.
  26. Lee, K.-S., Kim, G.-H., Seong, B.-J., Kim, H.-H., Kim, M.-Y. and Kim, M. R. 2009. Effects of aqueous medicinal herb extracts and aqueous fermented extracts on alcoholmetabolizing enzyme activities. Korean J. Food Preserv. 16, 259-265.
  27. Lee, S. O., Lee, H. J., Yu, M. H., Im, H. G. and Lee, I. S. 2005. Total polyphenol contents and antioxidant activities of methanol extracts from vegetables produced in Ulung Island. Korean J. Food Sci. Technol. 37, 233-243.
  28. Lim, S. M., Cheung, P. C. K. and Ooi, V. E. C. 2002. Evaluation of antioxidative activity of extracts from a brown seaweed, Sargassum siliquastrum. J. Agirc. Food Chem. 50, 3862-3866. https://doi.org/10.1021/jf020096b
  29. Matsukawa, R., Dubinsky, Z., Kishimoto, E., Masaki, K., Masuda, Y. and Takeuchi, T. 1997. A comparison of screening methods for antioxidant activity in seaweeds. J. Appl. Phycol. 9, 29-35 https://doi.org/10.1023/A:1007935218120
  30. Oh, J. K., Shin, Y. O., Shon, H. S. and Seo, R. M. 2003. Effect of functional food including seaweeds extracts supplementation on hematological variables and antioxidant system. Korean J. Physic. Edu. 42, 895-903.
  31. Park, J. W., Lee, J. H., Yoon, M. and Kim, C. H. 2001. Nitrite scavenging activity of the ethanol and water extracts obtained from Hizikia fusiforme and Sargassum fulvellum. Korean J. Life Sci. 11, 321-327.
  32. Tibbot, B. K. and Skadsen, R. W. 1996. Molecular cloning and characterization of a gibberellin-inducible, putative $\alpha$ -glucosidase gene from berley. Plant Mol. Biol. 30, 229-241. https://doi.org/10.1007/BF00020110
  33. Tottmar, S. O., Petterson, H. K. and Kiessling, H. 1973. The subcellular distribution and properties of aldehyde dehydrogenase in rat liver. Biochem. J. 135, 577-581.
  34. Yoon, S. Y., Lee, S. Y., Kim, K. B. W. R., Song, E. J., Lee, J., Lee, C. J., Park, N. B., Jung, J. Y., Kwak, J. H., Nam, K. W. and Ahn, D. H. 2010. Antimicrobial activity of the Sargassum fulvellum ethanol extract and the effect of temperature and pH on their activity. Korean J. Food Sci. Technol. 42, 155-159.

피인용 문헌

  1. Anti-inflammatory Effect of Ethanol Extract from Sargassum fulvellum on Lipopolysaccharide Induced Inflammatory Responses in RAW 264.7 Cells and Mice Ears vol.43, pp.8, 2014, https://doi.org/10.3746/jkfn.2014.43.8.1158
  2. Effect of Mixture Including Hot Water Extract of Houttuynia cordata Thunb on Ethanol-Induced Hangover in Rats vol.45, pp.10, 2016, https://doi.org/10.3746/jkfn.2016.45.10.1508
  3. The effect ofSargassum confusumon reduction of body fat in obese women vol.47, pp.1, 2014, https://doi.org/10.4163/jnh.2014.47.1.23
  4. Biological Analysis of Enzymatic Extracts from Sargassum fulvellum Using Polysaccharide Degrading Enzyme vol.28, pp.6, 2013, https://doi.org/10.7841/ksbbj.2013.28.6.349