생명과학회지 (Journal of Life Science)

  • 제22권10호
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  • Pages.1399-1406
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  • 2012
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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톳 분획물이 3T3-L1 지방전구세포의 분화 및 지방생성의 억제에 미치는 영향

Effects of Hizikia fusiforme Extracts on Adipocyte Differentiation and Adipogenesis in 3T3-L1 Preadipocytes

  • 최은옥 (동의대학교 생활과학대학 식품영양학과) ;
  • 김향숙 (동의대학교 생활과학대학 식품영양학과) ;
  • 한민호 (동의대학교 한의학과) ;
  • 최영현 (동의대학교 항노화연구소) ;
  • 김병우 (동의대학교 블루바이오소재개발센터) ;
  • 황진아 (명지대학교 식품영양학과) ;
  • 황혜진 (동의대학교 생활과학대학 식품영양학과)
  • Choi, Eun Ok (Department of Food and Nutrition, Dongeui University) ;
  • Kim, Hyang Suk (Department of Food and Nutrition, Dongeui University) ;
  • Han, Min Ho (Department of Oriental Medicine, Dongeui University) ;
  • Choi, Yung Hyun (Anti-aging Research Center, Dongeui University) ;
  • Kim, Byung Woo (Blue-Bio Industry Regional Innovation Center, Dongeui University) ;
  • Hwang, Jinah (Department of Food and Nutrition, Myongji University) ;
  • Hwang, Hye Jin (Department of Food and Nutrition, Dongeui University)
  • 투고 : 2012.08.31
  • 심사 : 2012.09.28
  • 발행 : 2012.10.30
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초록

본 연구에서는 톳 분획물의 항비만 효과 및 그에 따른 생화학적 기전의 해석을 위하여 톳 분획물이 비만유도인자에 의하여 인위적으로 유발된 adipogenesis 과정에 있어서 어떠한 영향을 미치는 지를 조사하였고, 이때 $PPAR{\gamma}$, C/$EBP{\alpha}$ 및 C/$EBP{\beta}$ 등과 같은 adipogenic transcription factor들의 발현에 어떠한 변화가 유발되었는지를 조사하였다. 각각의 톳 분획물들이 성숙한 지방세포에서 나타나는 lipid droplet 및 TG 생성에 어떠한 영향을 미치는 지를 확인한 결과, 모든 분획물에서 lipid droplet 및 TG 생성억제가 나타났지만 특히 WFHF 처리군에서 이러한 현상이 가장 강하게 나타났다. 또한 lipid droplet 및 TG 생성에 중요한 역할을 하는 것으로 알려진 adipogenic transcription factor들의발현에각각의분획물들이 어떠한영향을미치는지를확인한결과,WFHF 처리군에서 $PPAR{\gamma}$, C/$EBP{\alpha}$ 및 C/$EBP{\beta}$의 발현이 현저하게 감소하였음을 확인하였다. 이상의 결과를 종합해 보면 다섯 종류의 톳 분획물 모두 비만억제 효과가 있는 것으로 나타났고 특히 WFHF의 비만억제 효과가 강하게 나타났음을 알 수 있었다. 본 연구 결과는 톳의 비만억제 가능성을 제시하는 것으로서 항비만 기전에 대한 생화학적 해석 및 이를 활용한 향후 지속적인 연구를 위한 자료로서 그 가치가 매우 높을 것으로 생각된다.

The present study was conducted to evaluate the effects of various extracts of Hizikia fusiforme on the anti-obesity effects in 3T3-L1 preadipocytes. We used H. fusiforme extracts from ethanol (EEHF), dichloromethane (CFHF), ethyl acetate (EAFHF), butanol (BFHF), and water (WFHF). Treatment with these extracts significantly suppressed terminal differentiation of 3T3-L1 preadipocytes in a dose-dependent manner as confirmed by a decrease in lipid droplet content through Oil Red O staining; this effect was higher in WFHF than in other extracts. The concentrations of cellular triglyceride were also reduced in 3T3-L1 cells by exposure with these extracts, especially when compared with the controls. Treatment with 200 ${\mu}g/ml$ of WFHF and CFHF caused approximately 42.6% and 23.7% reduction, respectively. In addition, the extracts of H. fusiforme significantly reduced the expression levels of key pro-adipogenic transcription factors, including peroxisome proliferator-activated receptor ${\gamma}$ ($PPAR{\gamma}$) and CCAAT/enhancer binding proteins ${\alpha}$ (C/$EBP{\alpha}$) and C/$EBP{\beta}$ as compared with controls. Accordingly, our data indicated that WFHF has a preeminent effect on inhibition of adipocyte differentiation among various extracts, and H. fusiforme extracts may be an ideal candidate for obesity relief.

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