생명과학회지 (Journal of Life Science)

  • 제22권10호
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  • Pages.1384-1391
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  • 2012
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
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해안 생태계 복원을 위한 울릉도에 자생하는 해안식물의 뿌리로부터 분리된 내생진균류의 유전적 다양성 분석

Genetic Diversity of Endophytic Fungal Strains Isolated from the Roots of Coastal Plants in Ulleung Island for Restoration of Coastal Ecosystem

  • Kim, Miae (School of Life Sciences and Biotechnology, Kyungpook National University) ;
  • You, Young-Hyun (School of Life Sciences and Biotechnology, Kyungpook National University) ;
  • Yoon, Hyeokjun (School of Life Sciences and Biotechnology, Kyungpook National University) ;
  • Kim, Hyun (School of Life Sciences and Biotechnology, Kyungpook National University) ;
  • Seo, Yeonggyo (School of Life Sciences and Biotechnology, Kyungpook National University) ;
  • Khalmuratova, Irina (School of Life Sciences and Biotechnology, Kyungpook National University) ;
  • Shin, Jae-Ho (School of Applied Biosciences, Kyungpook National University) ;
  • Lee, In-Jung (School of Applied Biosciences, Kyungpook National University) ;
  • Choo, Yeon-Sik (Department of Biology, College of National Sciences, Kyungpook National University) ;
  • Kim, Jong-Guk (School of Life Sciences and Biotechnology, Kyungpook National University)
  • 투고 : 2012.08.21
  • 심사 : 2012.10.29
  • 발행 : 2012.10.30
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초록

본 연구에 사용된 해안식물은 울릉도의 해안지역으로부터 채집하였다. 그리고 식물은 큰비쑥, 해국, 갯질경이, 땅채송화, 갯강아지풀 등 5종류의 식물이 채집되었다. 울릉도에서 채집된 해안식물의 뿌리로부터 36주의 내생진균이 분리되었다. 모든 내생진균류는 ITS영역에 의해 분석 및 동정되었다. 그리고 계통분석 결과, 분리된 내생진균류는 모두 자낭균문에 속하고 4종류의 목(Capnodiales, Eurotiales, Hypocreales and Pleosporales)으로 분류되었으며, 이 중 Eurotiales 목이 가장 분포 비율이 높은 것으로 확인되었다. 그리고 내생진균류를 속으로 분류하였을 때, 9종류의 속(Alternaria, Aspergillus, Cladosporium, Exserohilum, Fusarium, Neosartorya, Penicillium, Phoma and Pyrenochaeta)으로 분류되었으며, Penicillium 속과 Aspergillus 속이 가장 우점종으로 확인되었다. 그리고 Shannon's diversity index (H')는 0.684에서 1.609의 분포로 나타났으며, 땅채송화에서 분리된 내생진균류의 다양성이 다른 식물에 비하여 높은 것으로 확인되었다.

Five coastal plant species, Artemisia fukudo, Aster sphathulifolius, Plantago camtschatica, Sedum oryzifolium, and Setaria viridis, were collected from the coastal region of Ulleung Island (Ulleung-Do, South Korea). Thirty-six endophytic fungi were isolated from the roots of these plants, and all were identified by using PCR with the following specifications: internal transcribed spacer 1 (ITS1), 5.8S rRNA, and ITS2 regions. Phylogenetic analysis indicated that all fungal strains belong to the phylum Ascomycota and comprise four orders (Capnodiales, Eurotiales, Hypocreales, and Pleosporales). Among all the identified species, the Eurotiales species were more abundant than species in the other orders. Nine different genera (Alternaria, Aspergillus, Cladosporium, Exserohilum, Fusarium, Neosartorya, Penicillium, Phoma, and Pyrenochaeta) in the four orders were confirmed. Penicillium and Aspergillus species were the most dominant species among the endophytic fungi isolated from the coastal plants. Shannon's diversity index (H') ranged from 0.684 to 1.609, and the endophytic fungi in S. oryzifolium was more diverse compared to the endophytic fungi in the other plants.

키워드

참고문헌

  1. Arnold, E. A., Mejia, L. C., Kyllo, D., Rojas, E., Maynard, Z., Robbins, N. and Herre, E. A. 2003. Fungal endophytes limit pathogen damage in a tropical tree. Proc. Natl. Acad. Sci. USA 100, 15649-15654. https://doi.org/10.1073/pnas.2533483100
  2. Chaillan, F., Fleche, A. L., Bury, E., Phantavong, Y., Grimont, P., `Saliot, A. and Oudot, J. 2004. Identification and biodegradation potential of tropical aerobic hydrocarbondegrading microorganisms. Res. Microbiol. 155, 587-595. https://doi.org/10.1016/j.resmic.2004.04.006
  3. Chapman, V. J. 1974. Salt Marshes and Salt Deserts of the World. Ecology of Halophytes. pp. 3-22, Academic Press, New York.
  4. Ding, B., Yin, Y., Zhang, F. and Li, Z. 2011. Recovery and phylogenetic diversity of culturable fungi associated with marine sponges Clathrina luteoculcitella and Holoxea sp. in the South China Sea. Mar. Biotechnol. 13, 713-721. https://doi.org/10.1007/s10126-010-9333-8
  5. El-Morsy, E. M. 2000. Fungi isolated from the endorhizosphere of halophytic plants from the Red Sea Coast of Egypt. Fungal Diversity 5, 43-54.
  6. Gomes, N. C. M., Cleary, D. F. R., Pinto, F. N., Egas, C., Almeida, A., Cunha, A., Mendonça-Hagler, L. C. S. and Smalla, K. 2010. Taking root: enduring effect of rhizosphere bacterial colonization in mangroves. PLoS ONE 5, e14065. https://doi.org/10.1371/journal.pone.0014065
  7. Gordon, W. L. 1965. Pathogenic strains of Fusarium oxysporum. Can. J. Bot. 43, 1309-1318. https://doi.org/10.1139/b65-138
  8. Hamayun, M., Khan, S. A., Khan, A. L., Rehman, G., Sohn, E. Y., Shah, A. A., Kim, S. K., Joo, G. J. and Lee, I. J. 2009. Phoma herbarum as a new gibberellin-producing and plant growth-promoting fungus. J. Microbiol. Biotechnol. 19, 1244-1249.
  9. Hamayun, M., Khan, S. A., Khan, M. A., Khan, A. L., Kang, S. M., Kim, S. K., Joo, G. J. and Lee, I. J. 2009. Gibberellin production by pure cultures of a new strain of Aspergillus fumigates. World J. Microbiol. Biotechnol. 25, 1785-1792. https://doi.org/10.1007/s11274-009-0078-3
  10. Hong, S. B., Go, S. J., Shin, H. D., Frisvad, J. C. and Samson, R. A. 2005. Polyphasic taxonomy of Aspergillus fumigatus and related species. Mycologia 97, 1316-1329 https://doi.org/10.3852/mycologia.97.6.1316
  11. Hong, S. B., Cho, H. S., Shin, H. D., Frisvad, J. C. and Samson, R. A. 2006. Novel Neosartorya species isolated from soil in Korea. Int J Systemat Evol Microbiol. 56, 477-486. https://doi.org/10.1099/ijs.0.63980-0
  12. Hong, S. B., Kim, D. H., Park, I. C., Samson, R. A. and Shin, H. D. 2010. Isolation and Identification of Aspergillus Section Fumigati Strains from Arable Soil in Korea. Mycobiology 38, 1-6. https://doi.org/10.4489/MYCO.2010.38.1.001
  13. Hwang, J. S., You, Y. H., Bae, J. J., Khan, S. A., Kim, J. G. and Choo, Y. S. 2011. Effects of endophytic fungal secondary metabolites on the growth and physiological response of Carex kobomugi Ohwi. J. Coastal Res. 27, 544-548. https://doi.org/10.2112/JCOASTRES-D-10-00090.1
  14. Kawaide, H. and Sassa, T. 1993. Accumulation of gibberellin $A_{1}$ and the metabolism of gibberellin $A_{9}$ to gibberellin $A_{1}$ in a Phaeosphaeria sp. L 487 culture. Biosci. Biotech. Biochem. 57, 1403-1405. https://doi.org/10.1271/bbb.57.1403
  15. Kawanabe, Y., Yamane, H., Murayama, T., Takahashi, N., and Nakamura, T. 1983. Identification of gibberellin A3 in mycelia Neurospora Crassa. Agric. Biol. Chem. 47, 1693-1694. https://doi.org/10.1271/bbb1961.47.1693
  16. Khan, S. A., Hamayun, M., Yoon, H. J., Kim, H. Y., Suh, S. J., Hwang, S. K., Kim, J. M., Lee, I. J., Choo, Y. S., Yoon, U. H., Kong, W. S., Lee, B. M. and Kim, J. G. 2008. Plant growth promotion and Penicillium citrinum. BMC Microbiol. 8, 231. https://doi.org/10.1186/1471-2180-8-231
  17. Khan, S. A., Hamayun, M., Kim, H. Y., Yoon, H. J., Lee, I. J. and Kim, J. G. 2009a. Gibberellin production and plant growth promotion by a newly isolated strain of Gliomastix murorum. World J. Microbiol. Biotechnol. 25, 829-833. https://doi.org/10.1007/s11274-009-9981-x
  18. Khan, S. A., Hamayun, M., Kim, H. Y., Yoon, H. J., Seo, J. C., Choo, Y. S., Lee, I. J., Kim, S. D., Rhee, I. K. and Kim, J. G. 2009b. A new strain of Arthrinium phaeospermum isolated from Carex kobomugi Ohwi is capable of gibberellin production. Biotechnol. Lett. 31, 283-287. https://doi.org/10.1007/s10529-008-9862-7
  19. Khan, A. L., Hamayun, M., Kang, S. M., Kim, Y. H., Jung, H. Y., Lee, J. H. and Lee, I. J. 2012. Endophytic fungal association via gibberellins and indole acetic acid can improve plant growth under abiotic stress: an example of Paecilomyces formosus LHL10. BMC Microbiol. 12, 3. https://doi.org/10.1186/1471-2180-12-3
  20. Kil, Y. J., Eo, J. K. and Eom, A. H. 2009. Molecular identification and diveristy of endophytic fungi isolated from Pinus densiflora in Boeun, Korea. Korean J. Mycol. 37, 130-133. https://doi.org/10.4489/KJM.2009.37.2.130
  21. Lee, H. B. and Yu, D. H. 1995. Distribution of mycotoxin-producing isolates in the Genus Alternaria. Korean J. Plant Pathol. 11, 151-157.
  22. Lee, J. H., Cho, H. J., Lee, B. C., Oh, S. H. and Bae, K. H. 2007. Forest vegetation types and growth characteristics of Seongin-bong in Ulleung Island, Korea. Korean J. Agric. For. Meteorol. 9, 37-48. https://doi.org/10.5532/KJAFM.2007.9.1.037
  23. Naranjo, L., Urbina, H., Sisto, A. D. and Leon, V. 2007. Isolation of autochthonous non-white rot fungi with potential for enzymatic upgrading of Venezuelan extra-heavy crude oil. Biocatal. Biotransformation 25, 341-349. https://doi.org/10.1080/10242420701379908
  24. Pielou, E. C. 1975. Ecological diversity. pp. 165, John Wiley, New York.
  25. Redman, R. S., Sheehan, K. B., Stout, R. G., Rodriguez, R. J. and Henson, J. M. 2002. Thermotolerance conferred to plant host and fungal endophyte during mutualistic symbiosis. Science 298, 1581. https://doi.org/10.1126/science.1072191
  26. Roberts, J. W. 1914. Experiments with apple leaf-spot fungi. J. Agr. Res. 2, 57-66.
  27. Rodriguez, R. J., Redman, R. S. and Henson, J. M. 2004. The role of fungal symbioses in the adaptation of plants to high stress environments. Mitigation and Adaptation Strategies for Global Change 9, 261-272. https://doi.org/10.1023/B:MITI.0000029922.31110.97
  28. Rodriguez, R. J., Henson, J., Van, V. E., Hoy, M., Wright, L., Beckwith, F., Kim, Y. and Redman, R. S. 2008. Stress tolerance in plants via habitat-adapted symbiosis. ISME J. 2, 404-416. https://doi.org/10.1038/ismej.2007.106
  29. Samson, R. A., Hong, S., Peterson, S. W., Frisvadand, J. C. and Varga, J. 2007. Polyphasic taxonomy of Aspergillus section Fumigati and its teleomorph Neosartorya. Stud. Mycol. 59, 147-203. https://doi.org/10.3114/sim.2007.59.14
  30. Sappapan, R., Sommit, D., Ngamrojanavanich, N., Pengpreecha, S., Wiyakrutta, S., Sriubolmas, N. and Pudhom, K. 2008. 11-Hydroxymonocerin from the plant endophytic fungus Exserohilum rostratum. J. Nat. Prod. 71, 1657-1659. https://doi.org/10.1021/np8004024
  31. Tamura, K., Dudley, J., Nei, M. and Kumar, S. 2007. Molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24, 1596-1599. https://doi.org/10.1093/molbev/msm092
  32. Tan, X. M., Chen, X. M., Wang, C. L., Jin, X. H., Cui, J. L., Chen, J., Guo, S. X. and Zhao, L. F. 2012. Isolation and identification of endophytic fungi in roots of nine Holcoglossum plants (Orchidaceae) collected from Yunnan, Guangxi, and Hainan provinces of China. Curr. Microbiol. 64, 140-147. https://doi.org/10.1007/s00284-011-0045-8
  33. Vazquez, M. M., Cesar, S., Azcon, R. and Barea, J. M. 2000. Interaction between arbuscular mycorrhizal fungi and other microbial inoculants (Azospirillum, Pseudomonas, Trichoderma) and their effects on microbial population and enzyme activities in the rhizosphere of maize plants. Appl. Soil Ecol. 15, 261-272. https://doi.org/10.1016/S0929-1393(00)00075-5
  34. Vishniac, H. S. 1996. Biodiversity of yeasts and filamentous microfungi in terrestrial Antarctic ecosystems. Biodivers. Conserv. 5, 1365-1378. https://doi.org/10.1007/BF00051983
  35. Waller, F., Achatz, B., Baltruscha, T. H., Fodor, J., Becker, K., Fischer, M., Heier, T., Hckelhoven, R., Neumann, C., Wettstein, D. V., Franken, P. and Kogel, K. H. 2005. The endophytic fungus Piriformospora indica reprograms barley to saltstress tolerance, disease resistance, and higher yield. Proc. Natl. Acad. Sci. USA 102, 13386-13391. https://doi.org/10.1073/pnas.0504423102
  36. Yamada, A., Takeo, O., Yosuke, D. and Masatake, O. 2001. Isolation of Tricholoma matsutake and T. bakamatsutake cultures from field-collected ectomycorrhizas. Mycoscience 42, 43-50. https://doi.org/10.1007/BF02463974
  37. Yang, X. B., Gao, X. D., Han, F. B. and Tan, R. X. 2005. Sulfation of a polysaccharide produced by a marine filamentous fungus Phoma herbarum YS4108 alters its antioxidant properties in vitro. Biochim. Biophys. Acta. Gen. Subj. 1725, 120-127. https://doi.org/10.1016/j.bbagen.2005.06.013
  38. Yi, T. W., Lee, E. H., Park, H. R. and Cho, K. S. 2011. Biodegradation of petroleum hydrocarbons by Neosartorya sp. BL4. J. Environ. Sci. Health A Tox. Hazard. Subst. Environ. Eng. 46, 1763-1768 https://doi.org/10.1080/10934529.2011.624004
  39. You, Y. H., Yoon, H., Lee, G. S., Woo, J. R., Shin, J. H., Lee, I. J., Rim, S. O., Choo, Y. S. and Kim, J. G. 2011. Diversity and plant growth-promotion of endophytic fungi isolated from the roots of plants in Dokdo islands. J. Life Sci. 21, 992-996. https://doi.org/10.5352/JLS.2011.21.7.992
  40. You, Y. H., Yoon, H. J., Woo, J. R., Seo, Y. G., Shin, J. H., Choo, Y. S., Lee, I. J. and Kim, J. G. 2011. Plant growth promotion activity of endophytic fungi isolated from the roots of Calystegia soldanella. Korean J. Microbiol. Biotechnol. 39, 324-329.
  41. You, Y. H., Yoon, Seo, H. Y., Kim, M., Kang, M. S., Kim, C. Ha, S. C., Cho, G. Y. and Kim, J. G. 2012. Genetic Diversity of Culturable Endophytic fungi Isolated from Halophytes Naturally Growing in Muan Salt Marsh. J. Life Sci. 22, 970-980. https://doi.org/10.5352/JLS.2012.22.7.970
  42. You, Y. H., Yoon, H., Woo, J. R., Seo, Y., Kim, M., Lee, G. and Kim, J. G. 2012. Diversity of Endophytic Fungi from the Roots of Halophytes Growing in Go-chang Salt Marsh. Korean J. Mycol. 40, 86-92. https://doi.org/10.4489/KJM.2012.40.2.86
  43. Zhang, X. Y., Bao, J. G., Wang, H., He, F., Xu, X. Y. and Qi, S. H. 2012. Diversity and Antimicrobial activity of culturable fungi isolated from six species of the South China sea gorgonians. Microbiol. Ecol. In press. DOI 10.1007/s00248-012-0050-x.

피인용 문헌

  1. Species List of Aspergillus, Penicillium and Talaromyces in Korea, Based on ‘One Fungus One Name’ System vol.44, pp.4, 2016, https://doi.org/10.4489/KJM.2016.44.4.207
  2. Culturable Fungal Endophytes Isolated from the Roots of Coastal Plants Inhabiting Korean East Coast vol.42, pp.2, 2014, https://doi.org/10.5941/MYCO.2014.42.2.100
  3. Metagenomic analysis of soil fungal communities on Ulleungdo and Dokdo Islands vol.61, pp.3, 2015, https://doi.org/10.2323/jgam.61.67
  4. Diversity Analysis of Endophytic Fungi Isolated from the Roots of Coastal Plants in Taean Peninsula vol.42, pp.1, 2014, https://doi.org/10.4489/KJM.2014.42.1.79
  5. Secondary metabolites from the endophytic fungus Talaromyces pinophilus vol.31, pp.15, 2017, https://doi.org/10.1080/14786419.2017.1290624
  6. Diversity and Plant Growth Promoting Capacity of Endophytic Fungi Associated with Halophytic Plants from the West Coast of Korea vol.43, pp.4, 2015, https://doi.org/10.5941/MYCO.2015.43.4.373