생명과학회지 (Journal of Life Science)

  • 제22권10호
  • /
  • Pages.1352-1358
  • /
  • 2012
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
DOI QR코드

DOI QR Code

흰쥐에서 내독소로 유도된 급성폐손상에서 surfactant내 PAF의 역할

PAF in Pulmonary Surfactant Contributes to Neutrophilic Oxidative Stress-Induced Acute Lung Injury of Rats Given LPS Intratracheally

  • 이영만 (대구가톨릭대학교 의과대학 생리학교실)
  • Lee, Young-Man (Department of Physiology, School of Medicine, Daegu Catholic University)
  • 투고 : 2012.07.27
  • 심사 : 2012.10.22
  • 발행 : 2012.10.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

흰쥐에서 대장균(E.coli : E0127;B8)의 내독소(lipopolysaccharide)로 급성 폐손상을 유도하고 이때 폐장 내 호중구성 respiratory burst에 따른 폐장조직의 변화 및 폐포 내로의 단백질 유출을 확인하였다. Pulmonary surfactant를 분리하고 이때 surfactant대사의 변화와 surfactant내의 PAF함량이 증가한 사실도 확인하였다. Surfactant내의 PAF함량의 증가는 폐포 내로의 호중구의 이동 및 그에 따른 산소기 생성의 증가로 폐장 내의 모세혈관 및 제1형 폐포세포의 직접적 손상의 원인으로 생각되었고, 이러한 surfactant내의 PAF의 증가가 실질적으로 급성 폐손상의 치료를 어렵게 하는 원인의 하나로 생각되었다.

In acute lung injury (ALI) by lipopolysaccharide (LPS), the underlying cause of infiltration and migration of neutrophils into the alveoli is considered to be from increased production of platelet-activating factor (PAF) in the pulmonary surfactant lining the alveolar lumen. In this study I partially confirmed this concept. LPS increased lung leak and the infiltration of neutrophils in the lung of rats given LPS intratracheally. The migration of neutrophils into the lung, which had caused oxidative stress, was also morphologically identified. I verified that the metabolism of the pulmonary surfactant was affected and that there was increased production of PAF in the pulmonary surfactant, both of which are considered to contribute to ALI by LPS in rats.

키워드

참고문헌

  1. Bligh, E. G. and Dyer, W. J. 1959. A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37, 911-917. https://doi.org/10.1139/o59-099
  2. Brown, R. E., Jarvis, J. L. and Hyland, K. J. 1989. Protein measurement using bicinchoninic acid: elimination of interfering substance. Anal. Biochem. 180, 136-139. https://doi.org/10.1016/0003-2697(89)90101-2
  3. Corbet, A., Cregan, J., Frink, J. and Rudolph, A. J. 1983. Distension-produced phospholipid secretion in postmortem in situ lungs of newborn rabbits: inhibition by specific beta- adrenergic blockade. Am. J. Respir. Dis. 128, 695-701.
  4. Goldblum, S. E., Wu, K. E. and Jay, M. 1985. Lung myeloperoxidase as a measurement of leukostasis in rabbits. J. Appl. Physiol. 119, L104-112.
  5. Hess, H. H. and Derr, J. E. 1975. Assay of inorganic and organic phosphorus in the 0.1-5 nanomolar range. Anal. Biochem. 63, 607-613. https://doi.org/10.1016/0003-2697(75)90388-7
  6. Hite, R. D., Seeds, M. C., Jacinto, R. B., Balasubramania, R. and Waite, M. 1998. Hydrolysis of surfactant associated phosphatidylcholine by mammalian secretory phospholipase A2. Am. J. Physiol. 275, L740-747.
  7. Hobson, J., Wright, J. and Churg, A. 1991. Histochemical evidence for generation of active oxygen species on the apical surface of cigarette-smoke-exposed tracheal explants. Am. J. Pathol. 139, 442-447.
  8. Holm, B. A., Keicher, L., Liu, M. A., Sokolowski, J. and Enhorning, G. 1991. Inhibition of pulmonary surfactant function by phospholipases. J. Appl. Physiol. 71, 317-321.
  9. Ikegami, M., Kallapur, S., Michna, J. and Jobe, A. H. 2000. Lung injury and surfactant metabolism after hyperventilation of premature lambs. Pediatr. Res. 47, 398-404. https://doi.org/10.1203/00006450-200003000-00019
  10. Kirsch, C. M., Brokaw, J. J., Prow, D. M. and White, G. W. 1992. Mechanism of platelet-activating factor-induced vascular leakage in the rat trachea. Exp. Lung Res. 18, 447-459. https://doi.org/10.3109/01902149209064339
  11. Lee, Y. M., Hybertson, B. M., Cho, H. G., Terada, L. S., Cho, O., Repine, A. J. and Repine, J. E. 2000. Platelet-activating factor contributes to acute lung leak in rats given interleukin-1 intratracheally. Am. J. Physiol. Lung Cell. Mol. Physiol. 279, L75-80.
  12. Lee, Y. M., Hybertson, B. M., Terada, L. S., Repine, A. J., Cho, H. G. and Repine, J. E. 1997. Mepacrine decreases lung leak in rats given interleukin-1 intratracheally. Am. Rev. Respir. Crit. Care Med. 155, 1624-1628. https://doi.org/10.1164/ajrccm.155.5.9154867
  13. Lee, Y. M., Hybertson, B. M., Cho, H. G. and Repine, J. E. 2002. Platelet-activating factor induces lung inflammation and leak in rats: hydrogen peroxide production along neutrophil- lung endothelial cell interfaces. J. Lab. Clin. Med. 140, 312-319. https://doi.org/10.1067/mlc.2002.128181
  14. Liau, D. F., Barret, C. R., Bell, A. L. and Ryan, S. F. 1987. Functional abnormalities of lung surfactant in experimental acute alveolar injury in the dog. Am. Rev. Repir. Dis. 136, 395-401. https://doi.org/10.1164/ajrccm/136.2.395
  15. Martensson, J. A., Jain, J. A., Frayer, W. and Meister, A. 1989. Glutathione metabolism in the lung: inhibition of its synthesis leads to lamellar body and mitochondrial defects. Proc. Natl. Acad. Sci. 86, 5296-5233. https://doi.org/10.1073/pnas.86.14.5296
  16. Minayama, Y., Tozawa, K., Kitamura, M., Saito, S. and Ogawa, J. 1998. Platelet-activating factor mediates intracellular adhesion molecule-1-dependent radical production in the non-hypoxic ischemia rat lung. Am. J. Cell. Mol. Biol. 19, 150-157. https://doi.org/10.1165/ajrcmb.19.1.3131
  17. Mitola, J. M., Jeffrey, P. K. and Hellewell, P. G. 1998. Platelet-activating factor plays a pivotal role in the induction of experimental lung injury. Am. J. Respir. Cell. Mol. Biol. 18, 197-204. https://doi.org/10.1165/ajrcmb.18.2.2846
  18. Moya, F. R., Hoffman, D. R., Zhao, B. and Johnston, J. M. 1993. Platelet-activating factor in pulmonary surfactant preparation. Lancet 341, 858-860. https://doi.org/10.1016/0140-6736(93)93062-6
  19. Nakos, G., Kitsiouli, E., Hatzidaki, E., Koulouras, V., Touqui, L. and Lekka, M. E. 2005. Phospholipase A2 and platelet- activating factor acetylhydrolase in patients with acute respiratory syndrome. Crit. Care Med. 33, 772-779. https://doi.org/10.1097/01.CCM.0000158519.80090.74
  20. Putman, E. J., Boere, F. Van Bree, L., Golde, L. M. G. and Haagman, H. P. 1995. Pulmonary surfactant subtype metabolism is altered after short-term ozone exposure. Toxicol. Appl. Pharmacol. 134, 132-138. https://doi.org/10.1006/taap.1995.1176
  21. Ribaldi, E., Mezzasoma, A. M., Francecangeli, E., Prosdicimi, M., Nenci, G. G. and Gresele, P. 1996. Inhibition of PAF synthesis by stimulated human polymorphonuclear leucocytes with cloricromene, an inhibitor of phospholipase A2 activation. British J. Pharmacol. 118, 1351-1358. https://doi.org/10.1111/j.1476-5381.1996.tb15544.x
  22. Shin, T. R., Na, B. K. and Lee, Y. M. 2005. Protective effect of the inhibition of PAF remodeling and adhesion molecule on the oxidative stress of the lungs of rats given endotoxin intratracheally. Tuberc. Respire. Dis 58, 276-284.
  23. Sorensen, J., Kald, B., Tagesson, C. and Lindahl, M. 1994. Platelet-activating factor and phospholipase A2 in patients with septic shock and trauma. Intensive Care Med. 20, 551-561.
  24. Triggiani, M., Marino, V. D., Sofia, M., Faraone, S., Ambrosio, G., Carratu, L. and Marone, G. 1997. Characterization of platelet-activating factor acetylhydrolase in human bronchoalveolar lavage. Am. J. Respir. Crit. Care Med. 156, 94-100. https://doi.org/10.1164/ajrccm.156.1.9608084