Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antioxidative Effects of Campanula takesimana Nakai Extract

섬초롱(Campanula takesimana Nakai) 용매 추출물의 항산화 효과

  • Kim, Mi-Seon (Dept. Of Food and Nutrition, Chungnam national University) ;
  • Kim, Kyoung-Hee (Dept. Of Food and Nutrition, Chungnam national University) ;
  • Yook, Hong-Sun (Dept. Of Food and Nutrition, Chungnam national University)
  • Received : 2012.06.27
  • Accepted : 2012.08.17
  • Published : 2012.10.31
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Abstract

The study analyzed the total polyphenolic contents, DPPH radical scavenging activities, ABTS radical scavenging activities, reducing power levels, SOD-like activities, and tyrosinase inhibition activities of fractions from Campanula takesimana Nakai extract. Total polyphenolic content of the ethyl acetate fraction from Campanula takesimana Nakai extract was the highest. The ethyl acetate fraction of Campanula takesimana Nakai extract showed the lowest $IC_{50}$ value (0.13 mg/mL) as well as greater than 98% ABTS radical scavenging activity, which was similar to ascorbic acid. Further, reducing power was significantly higher in the ethyl acetate fraction. The SOD-like activity of edible plant was 70.56% (n-butanol fraction) and 62.27% (ethyl acetate fraction) at 1 mg/mL. Tyrosinase inhibition activity of Campanula takesimana Nakai extract was reduced to 34.77% in the n-butanol fraction and 30.85% in the ethyl acetate fraction at 5 mg/mL. These results suggest that the ethyl acetate and n-butanol fractions of Campanula takesimana Nakai extract could be applicable as food additives or cosmeceutical ingredients.

본 연구는 섬초롱(Campanula takesimana Nakai)의 생리효능을 체계적으로 밝히기 위한 기초 자료를 얻고자 in vitro 에서 80% 에탄올 추출물과 순차적 분획물에 대하여 항산화 효과를 검증하였다. 섬초롱 에탄올 추출물의 추출 수율은 35.18%였으며, 이에 대하여 용매별로 분획한 수율은 water 분획물이 74.53%로 가장 높았으며, n-butanol, n-hexane, ethyl acetate, chloroform 순이었다. 섬초롱 추출물 및 분획물의 총 폴리페놀 함량은 ethyl acetate 분획물에서 276.26 mg/g으로 가장 높게 나타났다. DPPH radical 소거활성을 측정한 결과, 섬초롱 ethyl acetate 분획물에서 $IC_{50}$ 값이 0.13 mg/mL로 높은 활성을 나타내었다. 0.5 mg/mL 농도에서의 ABTS 라디칼 소거활성은 ethyl acetate 분획물에서 98% 이상의 활성을 보였으며, 이는 항산화 대조군인 ascorbic acid의 활성과 비교 시 높은 활성을 갖는 것으로 나타났다. 환원력은 분획물 모두 1 mg/mL 농도에서 ethyl acetate 분획물이 유의적으로 높은 흡광도 수치를 나타내었다. SOD 유사활성은 1 mg/mL의 농도로 측정한 결과, 섬초롱은 n-butanol 분획물 70.56%, ethyl acetate 분획물 62.27%로 나타났다. Tyrosinase 저해 활성은 5 mg/mL의 농도에서 n-butanol 분획물 34.77%, ethyl acetate 분획물 30.85% 순으로 나타났다. 이상의 결과로부터 섬초롱의 추출물 및 분획물 중 ethyl acetate와 n-butanol 분획물의 경우 높은 항산화 활성을 나타내어, 천연물소재로서 식품 첨가물 및 화장품 원료로의 가능성이 있는 것으로 사료된다.

Keywords

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