Antioxidant, Antimicrobial, and Cytotoxic Activities of Ovotransferrin from Egg White

  • Moon, Sun-Hee (Division of Animal Life Science and Bio/Moleucular Informatics Center, Konkuk University) ;
  • Lee, Jae-Hoon (Division of Animal Life Science and Bio/Moleucular Informatics Center, Konkuk University) ;
  • Lee, Yong-Ju (Division of Animal Life Science and Bio/Moleucular Informatics Center, Konkuk University) ;
  • Paik, Ji-Yeon (Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University) ;
  • Ahn, Dong-Uk (Department of Animal Science, Iowa State University) ;
  • Paik, Hyun-Dong (Division of Animal Life Science and Bio/Moleucular Informatics Center, Konkuk University)
  • Received : 2012.04.20
  • Accepted : 2012.10.10
  • Published : 2012.10.31


The antioxidant, antimicrobial, and cytotoxic activities of ovotransferrin were investigated in vitro. The antioxidant capacity of ovotransferrin was evaluated using the 2,2-Diphenyl-1-picryl hydrazyl (DPPH) radical scavenging method, antimicrobial effects using the agar well diffusion method, and cytotoxicity using the 3-(4,5-dimethylthizol-2-yl)-2,5-diphenylatetetrazolium bromide (MTT) assay. The DPPH radical-scavenging capacity of ovotransferrin at 1 mg/mL level reached approximately 60% after 48 h of reaction. The antimicrobial effects of ovotransferrin against common food-borne pathogens, Staphylococcus aureus KCCM 32395, Bacillus cereus KCCM 40935, Listeria monocytogenes ATCC 15313, Escherichia coli O157:H7 ATCC 43895, and Helicobacter pylori HpKCTC 26695 were dose dependant. Gram-positive bacteria was more sensitive to ovotransferrin than gram-negative bacteria. Ovotransferrin showed stronger antimicrobial effect against L. monocytogenes than other gram-positive bacteria tested. The cytotoxicity of ovotransferrin was evaluated in human cancer cell lines, various tissue origins, including the larynx (Hep-2), stomach (AGS), lung (SK-MES-1), liver (HepG2), breast (MCF-7), cervix (HeLa), and colon (HT-29). Ovotransferrin displayed relatively high cytotoxicity (${\leq}60%$ inhibition effects) at 40 mg/mL. At lower concentrations (${\leq}10mg/mL$), however, ovotransferrin cytotoxic effects were not significant in all cancer cell lines tested. These results indicated that ovotransferrin has potential to be used as an antioxidant or antimicrobial agent in foods or a pharmaceutical agent against cancers.



Supported by : National Research Foundation of Korea (NRF)


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