대한의생명과학회지 (Biomedical Science Letters)

  • 제18권3호
  • /
  • Pages.291-298
  • /
  • 2012
  • /
  • 1738-3226(pISSN)
  • /
  • 2288-7415(eISSN)
대한의생명과학회 (The Korean Society for Biomedical Laboratory Sciences)
권호 표지

Antiobesity Effect of Mixture of Black Garlic and Garsinia cambogia Extracts in 3T3-L1 Adipocytes and L6 Skeletal Muscle Cells

  • Jung, Young-Mi (Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University) ;
  • Lee, Dong-Sub (College of Health and Welfare, Kyungwoon University) ;
  • Lee, Seon-Ha (College of Health and Welfare, Kyungwoon University) ;
  • Jeoung, Nam-Ho (Department of Fundamental Medical & Pharmaceutical Sciences, CU-Leader's College, Catholic University of Daegu) ;
  • Kim, Bok-Jo (College of Health and Welfare, Kyungwoon University)
  • 투고 : 2012.09.06
  • 심사 : 2012.09.19
  • 발행 : 2012.09.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The antiobesity effect of the mixture of black garlic and Garsinia cambogia extracts (BGG) was investigated by measuring the Oil red O staining and the expressions of adipogenic genes during preadipocyte differentiation by real-time PCR in the 3T3-L1 adipocytes. BGG reduced contents of Oil red O dye in the 3T3-L1 adipocytes. mRNA expression levels of SREBP1c, C/EBPa, aP2/FABP4, and $PPAR{\gamma}$ which are adipogenic transcription factor, in cells treated with BGG were also significantly down regulated. Also, the phosphorylation of AMP-activated protein kinase (AMPK) in L6 cells was more increased by BGG. These results indicate that BGG seems to be more attractive compound for application of industry than individual extracts such as black garlic and Garsinia cambogia, considering it has two effects not only inhibit the preadipocyte differentiation but also activate the phosphorylation of AMPK unlike other two compound.

키워드

참고문헌

  1. Ankri S, Mirelman D. Antimicrobial properties of allicin from garlic. Microbes Infect. 1999. 1: 125-129. https://doi.org/10.1016/S1286-4579(99)80003-3
  2. Bakri IM, Douglas CW. Inhibitory effect of garlic extract on oral bacteria. Arch Oral Biol. 2005. 50: 645-651. https://doi.org/10.1016/j.archoralbio.2004.12.002
  3. Chauhan NB. Anti-amyloidogenic effect of Allium sativum in Alzheimer's transgenic model Tg2576. J Herb Pharmacother. 2003. 3: 95-107. https://doi.org/10.1080/J157v03n01_05
  4. Eljack AK, Hamm JK, Pilch PF, Farmer SR. Reconstitution of insulin-sensitive glucose transport in fibroblasts requires expression of both PPAR gamma and C/EBP alpha. J Biol Chem. 1999. 274: 7946-7951. https://doi.org/10.1074/jbc.274.12.7946
  5. Elmendorf JS, Chen D, Pessin JE. Guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S) stimulation of GLUT4 translocation is tyrosine kinase-dependent. J Biol Chem. 1998. 273: 13289-13296. https://doi.org/10.1074/jbc.273.21.13289
  6. Fox KE, Fankell DM, Erickson PF, Majka SM, Crossno Jr. JT, Klemm DJ. Depletion of cAMP-response element binding protein/ATF1 inhibits adipogenic conversion of 3T3-L1 cells ectopically expressing CCAAT/enhancer-binding protein (C/EBP) alpha, C/EBP beta, or PPAR gamma2. J Biol Chem. 2006. 281: 40341-40353. https://doi.org/10.1074/jbc.M605077200
  7. Goncagul G, Ayaz E. Antimicrobial effect of garlic (Allium sativum). Recent Pat Antiinfect Drug Discov. 1999. 5: 91-93.
  8. Hamm JK, Park BH, Farmer SR. A role for C/EBP beta in regulating peroxisome proliferator activated receptor gamma activity during adipogenesis in 3T3-L1 preadipocytes. J Biol Chem. 2001. 276: 18464-18471. https://doi.org/10.1074/jbc.M100797200
  9. Han SH, Sakuma I, Shin EK, Koh KK. Antiatherosclerotic and anti-insulin resistance effects of adiponectin: basic and clinical studies. Prog Cardiovasc Dis. 2009. 52: 126-140. https://doi.org/10.1016/j.pcad.2009.06.004
  10. Janderova L, McNeil M, Murrell AN, Mynatt RL, Smith SR. Human mesen chymal stem cells as an in vitro model for human adipogenesis. Obes Res. 2003. 11: 65-74. https://doi.org/10.1038/oby.2003.11
  11. Jang EK, Seo JH, Lee SP. Physiological activity and antioxidative effects of aged black garlic (Allium sativum L.) Extract. Korean J Food Sci Technol. 2008. 40: 443-448.
  12. Jung YM, Lee SH, Lee DS, You MJ, Chung IK, Cheon WH, Kwon YS, Lee YJ, Ku SK. Fermented garlic protects diabetic, obese mice when fed a high-fat diet by antioxidant effects. Nutrition Research. 2011. 31: 387-396. https://doi.org/10.1016/j.nutres.2011.04.005
  13. Kim IH, Jeong CH, Park SJ, Shim KH. Nutritional components and antioxidative activities of Jujube (Zizyphus jujuba) fruit and leaf. Korean J Food Preserv. 2011. 18: 341-348.
  14. Kim JM, Yoon MY, Choi SL, Kang IS, Kim SS, Kim YS, Choi YK, Ha JH. Effects of stimulation of AMP-activated protein kinase on insulin-like growth factor 1 and epidermal growth factor-dependent extracellular signal-regulated kinase pathway. J Biol Chem. 2001. 276: 19102-19110. https://doi.org/10.1074/jbc.M011579200
  15. Kim KY, Lee HN, Kim YJ, Park T. Garcinia cambogia extract ameliorates visceral adiposity in C57BL/6J mice fed on a high-fat diet. Biosci Biotechnol Biochem. 2008. 72: 1772-1780. https://doi.org/10.1271/bbb.80072
  16. Kook S, Kim GH, Choi K. The antidiabetic effect of onion and garlic in experimental diabetic rats: meta-analysis. J Med Food. 2009. 12: 552-560. https://doi.org/10.1089/jmf.2008.1071
  17. Mahmoodi M, Islami MR, Asadi Karam GR, Khaksari M, Sahebghadam Lotfi A, Hajizadeh MR, Mirzaee MR. Study of the effects of raw garlic consumption on the level of lipids and other blood biochemical factors in hyperlipidemic individuals. Pak J Pharm Sci. 2006. 19: 295-298.
  18. Mathew BC, Prasad NV, Prabodh R. Cholesterol-lowering effect of organosulphur compounds from garlic: a possible mechanism of action. Kathmandu Univ Med J. 2004. 2: 100-102.
  19. Park JA, Han MH, Kim BW, Chung YH, Choi YH. Inhibition of adipocyte differentiation and adipogenesis by aged black garlic extracts in 3T3-L1 preadipocytes, J. Life Science. 2011. 21: 720-728. https://doi.org/10.5352/JLS.2011.21.5.720
  20. Rajasree CR, Rajmohan T, Augusti KT. Antiatherogenic and antiperoxidative effects of garlic and soy proteins in alcohol fed rats. Indian J Exp Biol. 2009. 47: 169-175.
  21. Reinhart KM, Talati R, White CM, Coleman CI. The impact of garlic on lipid parameters: a systematic review and meta-analysis. Nutr Res Rev. 2009. 22: 39-48.
  22. Seki T, Hosono T, Hosono-Fukao T, Inada K, Tanaka R, Ogihara J, Ariga T. Anticancer effects of diallyl trisulfide derived from garlic. Asia Pac J Clin Nutr. 2008. 17: 249-252.
  23. Wang HC, Yang JH, Hsieh SC, Sheen LY. Allyl sulfides inhibit cell growth of skin cancer cells through induction of DNA damage mediated G2/M arrest and apoptosis. J Agric Food Chem. 2005. 58: 7096-7103.
  24. Ziemke F, Mantzoros CS. Adiponectin in insulin resistance: lessons from translational research. Am J Clin Nutr. 2007. 91: 258-261.