Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Diagnosis of canine brucellosis using recombinant ribosomal protein L7/L12

재조합 리보솜 단백질 L7/L12을 이용한 개 브루셀라병의 진단

  • Lee, Hyang-Keun (Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • Kim, Jong-Wan (Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • Ha, Yun-Mi (Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • Her, Moon (Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • Kim, Ji-Yeon (Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • Lee, Kichan (Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • Kang, Sung-Il (Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • Jung, Suk-Chan (Animal, Plant and Fisheries Quarantine and Inspection Agency)
  • 이향근 (농림수산검역검사본부 동물위생연구부 세균질병과) ;
  • 김종완 (농림수산검역검사본부 동물위생연구부 세균질병과) ;
  • 하윤미 (농림수산검역검사본부 동물위생연구부 세균질병과) ;
  • 허문 (농림수산검역검사본부 동물위생연구부 세균질병과) ;
  • 김지연 (농림수산검역검사본부 동물위생연구부 세균질병과) ;
  • 이기찬 (농림수산검역검사본부 동물위생연구부 세균질병과) ;
  • 강성일 (농림수산검역검사본부 동물위생연구부 세균질병과) ;
  • 정석찬 (농림수산검역검사본부 동물위생연구부 세균질병과)
  • Received : 2011.12.31
  • Accepted : 2012.02.27
  • Published : 2012.03.30
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Abstract

Brucella (B.) canis is mainly transmitted by direct or indirect contact with aborted fetuses and placenta. It's also known to be able to infect human, which likely results in providing veterinarians and companion animal owners for infectious risk. To develop diagnostic ELISA, we cloned and expressed rp1L gene of B. canis, which encodes the ribosomal protein L7/L12. Using this purified recombinant protein, indirect-ELISA (iELISA) was evaluated using 78 positive and 44 negative sera. The sensitivity and the specificity of iELISA were 94% and 89%, respectively. The results indicated that indirect-ELISA using recombinant ribosomal protein L7/L12 may be useful for diagnosis of canine brucellosis.

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